##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI Asp700I Asp718I AspEI
AspI AvaI BbvCI BciVI BclI
BglII BlpI Bpu1102I BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssSI Bst1107I
BstAPI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraIII EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT22I FbaI
FseI KpnI KspI MfeI MluI
MroI MspA1I MunI NdeI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmeI PshAI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SapI ScaI SgrAI SmaI SnaBI
SpeI SphI SspBI SspI SwaI
TfiI Tth111I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AfaI 1959 GTAC GT^AC
AflII 2108 CTTAAG C^TTAAG
AscI 106 GGCGCGCC GG^CGCGCC
AseI 2242 ATTAAT AT^TAAT
AsnI 2242 ATTAAT AT^TAAT
AviII 2297 TGCGCA TGC^GCA
AvrII 903 CCTAGG C^CTAGG
BamHI 1009 GGATCC G^GATCC
BbrPI 1951 CACGTG CAC^GTG
BbsI 518 GAAGAC GAAGAC(2/6)
BcgI 1385 GCATGGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2108 CTTAAG C^TTAAG
BlnI 903 CCTAGG C^CTAGG
BpuAI 518 GAAGAC GAAGAC(2/6)
BsaBI 1004 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmFI 1111 GTCCC GGGAC(10/14)
BssHII 107 GCGCGC G^CGCGC
BstBI 2090 TTCGAA TT^CGAA
DrdI 845 GACCTGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 388 GAATTC G^AATTC
FspI 2297 TGCGCA TGC^GCA
HindIII 1815 AAGCTT A^AGCTT
HinfI 2017 GAGTC G^A[ACGT]TC
HpaI 2167 GTTAAC GTT^AAC
MamI 1004 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1381 GCCGGC GCC^GGC
NgoMIV 1381 GCCGGC G^CCGGC
NheI 1768 GCTAGC G^CTAGC
NspI 489 ACATGC [GA]CATG^[CT]
NspV 2090 TTCGAA TT^CGAA
PleI 2017 GAGTC GAGTC(4/5)
PmaCI 1951 CACGTG CAC^GTG
PmlI 1951 CACGTG CAC^GTG
PshBI 2242 ATTAAT AT^TAAT
PstI 911 CTGCAG CTGCA^G
RsaI 1959 GTAC GT^AC
SbfI 910 CCTGCAGG CCTGCA^GG
SexAI 302 ACCTGGT A^CC[AT]GGT
SfcI 911 CTGCAG C^T[GA][CT]AG
SfiI 1291 GGCCCACTAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 2090 TTCGAA TT^CGAA
Sse8387I 910 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccII 30 CGCG CG^CG
108 CGCG
AcyI 1594 GGCGCC G[GA]^CG[CT]C
1672 GGCGCC
AlwNI 718 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
2361 CAGTTCCTG
AspHI 134 GAGCTC G[AT]GC[AT]^C
340 GAGCTC
BbeI 1594 GGCGCC GGCGC^C
1672 GGCGCC
BbvI 61 GCAGC GCAGC(8/12)
418 GCTGC
BcnI 139 CCGGG CC^[GC]GG
1013 CCGGG
BsaAI 334 TACGTG [CT]AC^GT[GA]
1951 CACGTG
BsaHI 1594 GGCGCC G[GA]^CG[CT]C
1672 GGCGCC
BsiHKAI 134 GAGCTC G[AT]GC[AT]^C
340 GAGCTC
BsmAI 776 GAGAC GTCTC(1/5)
2019 GTCTC
BspMI 909 ACCTGC ACCTGC(4/8)
1822 GCAGGT
BsrFI 1381 GCCGGC [GA]^CCGG[CT]
1576 ACCGGC
BstUI 30 CGCG CG^CG
108 CGCG
BstYI 474 AGATCC [GA]^GATC[CT]
1009 GGATCC
Cfr10I 1381 GCCGGC [GA]^CCGG[CT]
1576 ACCGGC
DraI 2125 TTTAAA TTT^AAA
2206 TTTAAA
FauI 5 CCCGC CCCGC(4/6)
1797 GCGGG
HgaI 28 GACGC GACGC(5/10)
352 GACGC
Hin1I 1594 GGCGCC G[GA]^CG[CT]C
1672 GGCGCC
HincII 2167 GTTAAC GT[CT]^[GA]AC
2328 GTTGAC
HindII 2167 GTTAAC GT[CT]^[GA]AC
2328 GTTGAC
KasI 1594 GGCGCC G^GCGCC
1672 GGCGCC
MflI 474 AGATCC [GA]^GATC[CT]
1009 GGATCC
MvnI 30 CGCG CG^CG
108 CGCG
NarI 1594 GGCGCC GG^CGCC
1672 GGCGCC
NciI 139 CCGGG CC^[GC]GG
1013 CCGGG
PflMI 1191 CCATGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1338 CCAGACCATGG
SacI 134 GAGCTC GAGCT^C
340 GAGCTC
SfoI 1594 GGCGCC GGC^GCC
1672 GGCGCC
SmlI 1645 CTTGAG C^T[CT][GA]AG
2108 CTTAAG
StuI 1299 AGGCCT AGG^CCT
1871 AGGCCT
TseI 61 GCAGC G^C[AT]GC
418 GCTGC
Tsp45I 291 GTCAC ^GT[GC]AC
664 GTGAC
Van91I 1191 CCATGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1338 CCAGACCATGG
XhoII 474 AGATCC [GA]^GATC[CT]
1009 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AcsI AluI AlwI ApaI
ApoI AvaII BalI BanI BanII
BfaI BglI BmyI BpmI BsaJI
BseRI BsiYI BslI Bsp1286I BsrBI
BsrDI BsrI BssKI BstF5I BstNI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspI
MvaI MwoI NcoI NdeII NlaIII
NlaIV PpuMI Sau3AI Sau96I ScrFI
SfaNI StyI TaqI Tru9I Tsp509I
TspRI TthHB8I XcmI