################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI Asp700I Asp718I AspEI AspI AvaI BbvCI BciVI BclI BglII BlpI Bpu1102I BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssSI Bst1107I BstAPI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI KpnI KspI MfeI MluI MroI MspA1I MunI NdeI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmeI PshAI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI ScaI SgrAI SmaI SnaBI SpeI SphI SspBI SspI SwaI TfiI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AfaI 1959 GTAC GT^AC AflII 2108 CTTAAG C^TTAAG AscI 106 GGCGCGCC GG^CGCGCC AseI 2242 ATTAAT AT^TAAT AsnI 2242 ATTAAT AT^TAAT AviII 2297 TGCGCA TGC^GCA AvrII 903 CCTAGG C^CTAGG BamHI 1009 GGATCC G^GATCC BbrPI 1951 CACGTG CAC^GTG BbsI 518 GAAGAC GAAGAC(2/6) BcgI 1385 GCATGGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 2108 CTTAAG C^TTAAG BlnI 903 CCTAGG C^CTAGG BpuAI 518 GAAGAC GAAGAC(2/6) BsaBI 1004 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmFI 1111 GTCCC GGGAC(10/14) BssHII 107 GCGCGC G^CGCGC BstBI 2090 TTCGAA TT^CGAA DrdI 845 GACCTGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 388 GAATTC G^AATTC FspI 2297 TGCGCA TGC^GCA HindIII 1815 AAGCTT A^AGCTT HinfI 2017 GAGTC G^A[ACGT]TC HpaI 2167 GTTAAC GTT^AAC MamI 1004 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 1381 GCCGGC GCC^GGC NgoMIV 1381 GCCGGC G^CCGGC NheI 1768 GCTAGC G^CTAGC NspI 489 ACATGC [GA]CATG^[CT] NspV 2090 TTCGAA TT^CGAA PleI 2017 GAGTC GAGTC(4/5) PmaCI 1951 CACGTG CAC^GTG PmlI 1951 CACGTG CAC^GTG PshBI 2242 ATTAAT AT^TAAT PstI 911 CTGCAG CTGCA^G RsaI 1959 GTAC GT^AC SbfI 910 CCTGCAGG CCTGCA^GG SexAI 302 ACCTGGT A^CC[AT]GGT SfcI 911 CTGCAG C^T[GA][CT]AG SfiI 1291 GGCCCACTAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 2090 TTCGAA TT^CGAA Sse8387I 910 CCTGCAGG CCTGCA^GG ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AccII 30 CGCG CG^CG 108 CGCG AcyI 1594 GGCGCC G[GA]^CG[CT]C 1672 GGCGCC AlwNI 718 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG 2361 CAGTTCCTG AspHI 134 GAGCTC G[AT]GC[AT]^C 340 GAGCTC BbeI 1594 GGCGCC GGCGC^C 1672 GGCGCC BbvI 61 GCAGC GCAGC(8/12) 418 GCTGC BcnI 139 CCGGG CC^[GC]GG 1013 CCGGG BsaAI 334 TACGTG [CT]AC^GT[GA] 1951 CACGTG BsaHI 1594 GGCGCC G[GA]^CG[CT]C 1672 GGCGCC BsiHKAI 134 GAGCTC G[AT]GC[AT]^C 340 GAGCTC BsmAI 776 GAGAC GTCTC(1/5) 2019 GTCTC BspMI 909 ACCTGC ACCTGC(4/8) 1822 GCAGGT BsrFI 1381 GCCGGC [GA]^CCGG[CT] 1576 ACCGGC BstUI 30 CGCG CG^CG 108 CGCG BstYI 474 AGATCC [GA]^GATC[CT] 1009 GGATCC Cfr10I 1381 GCCGGC [GA]^CCGG[CT] 1576 ACCGGC DraI 2125 TTTAAA TTT^AAA 2206 TTTAAA FauI 5 CCCGC CCCGC(4/6) 1797 GCGGG HgaI 28 GACGC GACGC(5/10) 352 GACGC Hin1I 1594 GGCGCC G[GA]^CG[CT]C 1672 GGCGCC HincII 2167 GTTAAC GT[CT]^[GA]AC 2328 GTTGAC HindII 2167 GTTAAC GT[CT]^[GA]AC 2328 GTTGAC KasI 1594 GGCGCC G^GCGCC 1672 GGCGCC MflI 474 AGATCC [GA]^GATC[CT] 1009 GGATCC MvnI 30 CGCG CG^CG 108 CGCG NarI 1594 GGCGCC GG^CGCC 1672 GGCGCC NciI 139 CCGGG CC^[GC]GG 1013 CCGGG PflMI 1191 CCATGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1338 CCAGACCATGG SacI 134 GAGCTC GAGCT^C 340 GAGCTC SfoI 1594 GGCGCC GGC^GCC 1672 GGCGCC SmlI 1645 CTTGAG C^T[CT][GA]AG 2108 CTTAAG StuI 1299 AGGCCT AGG^CCT 1871 AGGCCT TseI 61 GCAGC G^C[AT]GC 418 GCTGC Tsp45I 291 GTCAC ^GT[GC]AC 664 GTGAC Van91I 1191 CCATGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1338 CCAGACCATGG XhoII 474 AGATCC [GA]^GATC[CT] 1009 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 78 enzymes) # ######################################### AciI AcsI AluI AlwI ApaI ApoI AvaII BalI BanI BanII BfaI BglI BmyI BpmI BsaJI BseRI BsiYI BslI Bsp1286I BsrBI BsrDI BsrI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspI MvaI MwoI NcoI NdeII NlaIII NlaIV PpuMI Sau3AI Sau96I ScrFI SfaNI StyI TaqI Tru9I Tsp509I TspRI TthHB8I XcmI