##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccI AflII AgeI
AhdI Alw44I AocI ApaLI AscI
Asp718I AspEI AspI AvaI AviII
BamHI BbeI BbvCI BcgI BfrI
BglII BsaBI BsiEI BsiWI BsoBI
BspDI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraIII EagI
Eam1105I EclXI Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HindIII HpaI KasI KpnI KspI
MamI MfeI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PaeR7I PflFI PinAI
PmeI PshAI PvuI RsrII SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccII 2128 CGCG CG^CG
AccIII 1639 TCCGGA T^CCGGA
AcsI 2986 AAATTT [GA]^AATT[CT]
ApoI 2986 AAATTT [GA]^AATT[CT]
AseI 2902 ATTAAT AT^TAAT
AsnI 2902 ATTAAT AT^TAAT
AvrII 1853 CCTAGG C^CTAGG
BalI 2095 TGGCCA TGG^CCA
BciVI 421 GTATCC GTATCC(6/5)
BclI 391 TGATCA T^GATCA
BglI 1503 GCCGGAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1853 CCTAGG C^CTAGG
BsaI 843 GAGACC GGTCTC(1/5)
BsaWI 1639 TCCGGA [AT]^CCGG[AT]
BseAI 1639 TCCGGA T^CCGGA
BsmI 2582 GCATTC GAATGC(1/-1)
Bsp1407I 2768 TGTACA T^GTACA
BspEI 1639 TCCGGA T^CCGGA
BspHI 1813 TCATGA T^CATGA
BsrDI 2605 GCAATG GCAATG(2/0)
BsrGI 2768 TGTACA T^GTACA
BstUI 2128 CGCG CG^CG
DrdI 2791 GACTATTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 391 TGATCA T^GATCA
HincII 539 GTCAAC GT[CT]^[GA]AC
HindII 539 GTCAAC GT[CT]^[GA]AC
MluI 2127 ACGCGT A^CGCGT
MluNI 2095 TGGCCA TGG^CCA
MroI 1639 TCCGGA T^CCGGA
MscI 2095 TGGCCA TGG^CCA
MvnI 2128 CGCG CG^CG
NcoI 2085 CCATGG C^CATGG
PacI 2903 TTAATTAA TTAAT^TAA
PflMI 2080 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 2902 ATTAAT AT^TAAT
RcaI 1813 TCATGA T^CATGA
SapI 779 GAAGAGC GCTCTTC(1/4)
ScaI 2954 AGTACT AGT^ACT
SexAI 685 ACCTGGT A^CC[AT]GGT
SmlI 2888 CTTGAG C^T[CT][GA]AG
SspBI 2768 TGTACA T^GTACA
TfiI 1726 GATTC G^A[AT]TC
Van91I 2080 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AclI 1428 AACGTT AA^CGTT
2472 AACGTT
AcyI 2436 GACGCC G[GA]^CG[CT]C
2564 GACGCC
Aor51HI 1780 AGCGCT AGC^GCT
1807 AGCGCT
AspHI 1201 GAGCTC G[AT]GC[AT]^C
2065 GAGCTC
BbrPI 1547 CACGTG CAC^GTG
1605 CACGTG
BbsI 1631 GAAGAC GAAGAC(2/6)
1643 GAAGAC
BpmI 1746 CTCCAG CTGGAG(16/14)
352 CTGGAG
BpuAI 1631 GAAGAC GAAGAC(2/6)
1643 GAAGAC
BsaAI 1547 CACGTG [CT]AC^GT[GA]
1605 CACGTG
BsaHI 2436 GACGCC G[GA]^CG[CT]C
2564 GACGCC
BsiHKAI 1201 GAGCTC G[AT]GC[AT]^C
2065 GAGCTC
BsmBI 828 GAGACG CGTCTC(1/5)
2109 GAGACG
BspLU11I 156 ACATGT A^CATGT
2807 ACATGT
DraI 2813 TTTAAA TTT^AAA
2846 TTTAAA
EaeI 58 TGGCCG [CT]^GGCC[GA]
2095 TGGCCA
EarI 779 GAAGAG CTCTTC(1/4)
1574 GAAGAG
Eco47III 1780 AGCGCT AGC^GCT
1807 AGCGCT
HaeII 1780 AGCGCT [GA]GCGC^[CT]
1807 AGCGCT
Hin1I 2436 GACGCC G[GA]^CG[CT]C
2564 GACGCC
Ksp632I 779 GAAGAG CTCTTC(1/4)
1574 GAAGAG
PmaCI 1547 CACGTG CAC^GTG
1605 CACGTG
PmlI 1547 CACGTG CAC^GTG
1605 CACGTG
PpuMI 2134 GGGACCC [GA]G^G[AT]CC[CT]
2443 AGGACCT
Psp1406I 1428 AACGTT AA^CGTT
2472 AACGTT
SacI 1201 GAGCTC GAGCT^C
2065 GAGCTC
SfaNI 289 GCATC GCATC(5/9)
1291 GCATC
TaqI 1760 TCGA T^CGA
2175 TCGA
TthHB8I 1760 TCGA T^CGA
2175 TCGA
XcmI 1951 CCACGACTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2347 CCACCGGGGAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI ApaI Asp700I AvaII BanI
BanII BbvI BcnI BfaI BlpI
BmyI Bpu1102I BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrBI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfcI StyI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII XmnI