################################## # NO CUT ENZYMES ( 94 enzymes) # ################################## AatII Acc65I AccI AflII AgeI AhdI Alw44I AocI ApaLI AscI Asp718I AspEI AspI AvaI AviII BamHI BbeI BbvCI BcgI BfrI BglII BsaBI BsiEI BsiWI BsoBI BspDI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I Cfr10I ClaI CpoI DraIII EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HindIII HpaI KasI KpnI KspI MamI MfeI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PaeR7I PflFI PinAI PmeI PshAI PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccII 2128 CGCG CG^CG AccIII 1639 TCCGGA T^CCGGA AcsI 2986 AAATTT [GA]^AATT[CT] ApoI 2986 AAATTT [GA]^AATT[CT] AseI 2902 ATTAAT AT^TAAT AsnI 2902 ATTAAT AT^TAAT AvrII 1853 CCTAGG C^CTAGG BalI 2095 TGGCCA TGG^CCA BciVI 421 GTATCC GTATCC(6/5) BclI 391 TGATCA T^GATCA BglI 1503 GCCGGAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 1853 CCTAGG C^CTAGG BsaI 843 GAGACC GGTCTC(1/5) BsaWI 1639 TCCGGA [AT]^CCGG[AT] BseAI 1639 TCCGGA T^CCGGA BsmI 2582 GCATTC GAATGC(1/-1) Bsp1407I 2768 TGTACA T^GTACA BspEI 1639 TCCGGA T^CCGGA BspHI 1813 TCATGA T^CATGA BsrDI 2605 GCAATG GCAATG(2/0) BsrGI 2768 TGTACA T^GTACA BstUI 2128 CGCG CG^CG DrdI 2791 GACTATTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FbaI 391 TGATCA T^GATCA HincII 539 GTCAAC GT[CT]^[GA]AC HindII 539 GTCAAC GT[CT]^[GA]AC MluI 2127 ACGCGT A^CGCGT MluNI 2095 TGGCCA TGG^CCA MroI 1639 TCCGGA T^CCGGA MscI 2095 TGGCCA TGG^CCA MvnI 2128 CGCG CG^CG NcoI 2085 CCATGG C^CATGG PacI 2903 TTAATTAA TTAAT^TAA PflMI 2080 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 2902 ATTAAT AT^TAAT RcaI 1813 TCATGA T^CATGA SapI 779 GAAGAGC GCTCTTC(1/4) ScaI 2954 AGTACT AGT^ACT SexAI 685 ACCTGGT A^CC[AT]GGT SmlI 2888 CTTGAG C^T[CT][GA]AG SspBI 2768 TGTACA T^GTACA TfiI 1726 GATTC G^A[AT]TC Van91I 2080 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AclI 1428 AACGTT AA^CGTT 2472 AACGTT AcyI 2436 GACGCC G[GA]^CG[CT]C 2564 GACGCC Aor51HI 1780 AGCGCT AGC^GCT 1807 AGCGCT AspHI 1201 GAGCTC G[AT]GC[AT]^C 2065 GAGCTC BbrPI 1547 CACGTG CAC^GTG 1605 CACGTG BbsI 1631 GAAGAC GAAGAC(2/6) 1643 GAAGAC BpmI 1746 CTCCAG CTGGAG(16/14) 352 CTGGAG BpuAI 1631 GAAGAC GAAGAC(2/6) 1643 GAAGAC BsaAI 1547 CACGTG [CT]AC^GT[GA] 1605 CACGTG BsaHI 2436 GACGCC G[GA]^CG[CT]C 2564 GACGCC BsiHKAI 1201 GAGCTC G[AT]GC[AT]^C 2065 GAGCTC BsmBI 828 GAGACG CGTCTC(1/5) 2109 GAGACG BspLU11I 156 ACATGT A^CATGT 2807 ACATGT DraI 2813 TTTAAA TTT^AAA 2846 TTTAAA EaeI 58 TGGCCG [CT]^GGCC[GA] 2095 TGGCCA EarI 779 GAAGAG CTCTTC(1/4) 1574 GAAGAG Eco47III 1780 AGCGCT AGC^GCT 1807 AGCGCT HaeII 1780 AGCGCT [GA]GCGC^[CT] 1807 AGCGCT Hin1I 2436 GACGCC G[GA]^CG[CT]C 2564 GACGCC Ksp632I 779 GAAGAG CTCTTC(1/4) 1574 GAAGAG PmaCI 1547 CACGTG CAC^GTG 1605 CACGTG PmlI 1547 CACGTG CAC^GTG 1605 CACGTG PpuMI 2134 GGGACCC [GA]G^G[AT]CC[CT] 2443 AGGACCT Psp1406I 1428 AACGTT AA^CGTT 2472 AACGTT SacI 1201 GAGCTC GAGCT^C 2065 GAGCTC SfaNI 289 GCATC GCATC(5/9) 1291 GCATC TaqI 1760 TCGA T^CGA 2175 TCGA TthHB8I 1760 TCGA T^CGA 2175 TCGA XcmI 1951 CCACGACTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2347 CCACCGGGGAGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 92 enzymes) # ######################################### AciI AfaI AflIII AluI AlwI AlwNI ApaI Asp700I AvaII BanI BanII BbvI BcnI BfaI BlpI BmyI Bpu1102I BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrBI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfcI StyI Tru9I TseI Tsp45I Tsp509I TspRI XhoII XmnI