##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AccII AccIII AclI AflII AgeI
AhdI AscI Asp700I AspEI AviII
BamHI BbeI BfrI BglI BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BspDI BspEI BspLU11I
BsrBI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Cfr10I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco52I EcoO65I
FseI FspI HpaI KasI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NgoMIV NheI
NotI NruI NspV PacI PinAI
PmeI PshAI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SwaI XbaI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 793 GACGTC GACGT^C
Acc65I 1091 GGTACC G^GTACC
AccI 99 GTCTAC GT^[AC][GT]AC
AflIII 1859 ACACGT A^C[GA][CT]GT
AocI 3636 CCTGAGG CC^T[ACGT]AGG
Aor51HI 3372 AGCGCT AGC^GCT
ApaI 2573 GGGCCC GGGCC^C
AseI 2566 ATTAAT AT^TAAT
AsnI 2566 ATTAAT AT^TAAT
Asp718I 1091 GGTACC G^GTACC
AspI 93 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3417 CCTAGG C^CTAGG
BanI 1091 GGTACC G^G[CT][GA]CC
BbvCI 456 CCTCAGC CCTCAGC(-5/-2)
BcgI 1745 GCACAATCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2558 GTATCC GTATCC(6/5)
BclI 86 TGATCA T^GATCA
BcnI 624 CCGGG CC^[GC]GG
BglII 3404 AGATCT A^GATCT
BlnI 3417 CCTAGG C^CTAGG
Bsp1407I 2714 TGTACA T^GTACA
BspHI 1778 TCATGA T^CATGA
BsrGI 2714 TGTACA T^GTACA
BstXI 3182 CCAAGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3636 CCTGAGG CC^T[ACGT]AGG
DsaI 2477 CCATGG C^C[GA][CT]GG
Eco47III 3372 AGCGCT AGC^GCT
Eco81I 3636 CCTGAGG CC^T[ACGT]AGG
EcoRV 1939 GATATC GAT^ATC
EcoT22I 1789 ATGCAT ATGCA^T
FauI 3589 CCCGC CCCGC(4/6)
FbaI 86 TGATCA T^GATCA
HindIII 3504 AAGCTT A^AGCTT
KpnI 1091 GGTACC GGTAC^C
NciI 624 CCGGG CC^[GC]GG
NcoI 2477 CCATGG C^CATGG
NdeI 3085 CATATG CA^TATG
NsiI 1789 ATGCAT ATGCA^T
PaeR7I 261 CTCGAG C^TCGAG
PflFI 93 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2310 CCAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 2566 ATTAAT AT^TAAT
RcaI 1778 TCATGA T^CATGA
SexAI 2741 ACCTGGT A^CC[AT]GGT
SspBI 2714 TGTACA T^GTACA
Tth111I 93 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2310 CCAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 261 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 793 GACGTC G[GA]^CG[CT]C
1657 GGCGTC
Alw44I 2939 GTGCAC G^TGCAC
3667 GTGCAC
ApaLI 2939 GTGCAC G^TGCAC
3667 GTGCAC
AvaI 66 CTCGGG C^[CT]CG[GA]G
261 CTCGAG
BlpI 1984 GCTCAGC GC^T[ACGT]AGC
2996 GCTCAGC
Bpu1102I 1984 GCTCAGC GC^T[ACGT]AGC
2996 GCTCAGC
BsaHI 793 GACGTC G[GA]^CG[CT]C
1657 GGCGTC
BsoBI 66 CTCGGG C^[CT]CG[GA]G
261 CTCGAG
BstAPI 964 GCAGACAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3660 GCACGTGGTGC
CelII 1984 GCTCAGC GC^T[ACGT]AGC
2996 GCTCAGC
EcoNI 1617 CCTTAATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3557 CCTTCCAGAGG
EcoRI 848 GAATTC G^AATTC
1323 GAATTC
HapII 624 CCGG C^CGG
2586 CCGG
HgaI 912 GCGTC GACGC(5/10)
1658 GCGTC
Hin1I 793 GACGTC G[GA]^CG[CT]C
1657 GGCGTC
HincII 948 GTCAAC GT[CT]^[GA]AC
1197 GTTGAC
HindII 948 GTCAAC GT[CT]^[GA]AC
1197 GTTGAC
HpaII 624 CCGG C^CGG
2586 CCGG
MspI 624 CCGG C^CGG
2586 CCGG
PpuMI 1111 GGGACCC [GA]G^G[AT]CC[CT]
1428 AGGTCCT
PstI 1186 CTGCAG CTGCA^G
2854 CTGCAG
PvuII 20 CAGCTG CAG^CTG
3381 CAGCTG
SacI 1396 GAGCTC GAGCT^C
2731 GAGCTC
SspI 746 AATATT AAT^ATT
1492 AATATT
StuI 2002 AGGCCT AGG^CCT
3263 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BalI
BanII BbrPI BbsI BbvI BfaI
BmyI BpmI BpuAI BsaAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DraIII EaeI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HhaI HinP1I HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PmaCI PmlI RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII