##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII AclI AflII AgeI Alw44I
Aor51HI ApaLI AseI AsnI Asp700I
AviII AvrII BalI BamHI BciVI
BfrI BlnI BsaBI BsaI BsiWI
BsmBI Bsp1407I BspDI BsrBI BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DrdI EagI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HincII
HindII HpaI KspI MamI MfeI
MluI MluNI MscI MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PinAI PpuMI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI XbaI XcmI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 2200 GGTACC G^GTACC
AccI 2252 GTCTAC GT^[AC][GT]AC
AccIII 110 TCCGGA T^CCGGA
AcsI 2918 AAATTT [GA]^AATT[CT]
AcyI 104 GGCGCC G[GA]^CG[CT]C
AhdI 959 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 344 GGGCCC GGGCC^C
ApoI 2918 AAATTT [GA]^AATT[CT]
AscI 162 GGCGCGCC GG^CGCGCC
Asp718I 2200 GGTACC G^GTACC
AspEI 959 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2108 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 802 CTCGAG C^[CT]CG[GA]G
BbeI 104 GGCGCC GGCGC^C
BbrPI 136 CACGTG CAC^GTG
BbvCI 443 CCTCAGC CCTCAGC(-5/-2)
BcgI 205 CGAGCCCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2400 TGATCA T^GATCA
BglII 1669 AGATCT A^GATCT
BsaAI 136 CACGTG [CT]AC^GT[GA]
BsaHI 104 GGCGCC G[GA]^CG[CT]C
BsaWI 110 TCCGGA [AT]^CCGG[AT]
BseAI 110 TCCGGA T^CCGGA
BsiEI 184 CGACCG CG[GA][CT]^CG
BsoBI 802 CTCGAG C^[CT]CG[GA]G
BspEI 110 TCCGGA T^CCGGA
BsrFI 322 GCCGGC [GA]^CCGG[CT]
BssHII 163 GCGCGC G^CGCGC
Cfr10I 322 GCCGGC [GA]^CCGG[CT]
DraI 2481 TTTAAA TTT^AAA
DsaI 120 CCACGG C^C[GA][CT]GG
EaeI 1995 CGGCCA [CT]^GGCC[GA]
Eam1105I 959 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2644 CCTTTCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 2400 TGATCA T^GATCA
Hin1I 104 GGCGCC G[GA]^CG[CT]C
HindIII 1936 AAGCTT A^AGCTT
KasI 104 GGCGCC G^GCGCC
KpnI 2200 GGTACC GGTAC^C
MroI 110 TCCGGA T^CCGGA
NaeI 322 GCCGGC GCC^GGC
NarI 104 GGCGCC GG^CGCC
NgoMIV 322 GCCGGC G^CCGGC
PaeR7I 802 CTCGAG C^TCGAG
PflFI 2108 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 136 CACGTG CAC^GTG
PmeI 2480 GTTTAAAC GTTT^AAAC
PmlI 136 CACGTG CAC^GTG
PshAI 1880 GACTACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfiI 432 GGCCCCATCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 104 GGCGCC GGC^GCC
SphI 2553 GCATGC GCATG^C
Tth111I 2108 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 802 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 840 ACATGT A^C[GA][CT]GT
1101 ACATGT
AlwNI 568 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
2244 CAGATCCTG
AvaII 835 GGTCC G^G[AT]CC
1197 GGACC
BbsI 622 GAAGAC GAAGAC(2/6)
677 GAAGAC
BglI 433 GCCCCATCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1751 GCCCTCAAGGC
BlpI 543 GCTCAGC GC^T[ACGT]AGC
1896 GCTCAGC
Bpu1102I 543 GCTCAGC GC^T[ACGT]AGC
1896 GCTCAGC
BpuAI 622 GAAGAC GAAGAC(2/6)
677 GAAGAC
BspHI 1080 TCATGA T^CATGA
2064 TCATGA
BspLU11I 840 ACATGT A^CATGT
1101 ACATGT
BspMI 81 GCAGGT ACCTGC(4/8)
2395 GCAGGT
BsrDI 1789 CATTGC GCAATG(2/0)
903 GCAATG
CelII 543 GCTCAGC GC^T[ACGT]AGC
1896 GCTCAGC
DraIII 133 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
1307 CACAGAGTG
HgaI 198 GACGC GACGC(5/10)
540 GACGC
PflMI 1306 CCACAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2500 CCAAGGCATGG
PstI 1163 CTGCAG CTGCA^G
1874 CTGCAG
PvuII 66 CAGCTG CAG^CTG
1265 CAGCTG
RcaI 1080 TCATGA T^CATGA
2064 TCATGA
SacI 1253 GAGCTC GAGCT^C
1850 GAGCTC
SexAI 34 ACCTGGT A^CC[AT]GGT
2513 ACCTGGT
Van91I 1306 CCACAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2500 CCAAGGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AocI AspHI BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII