##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII AccII AccIII AclI AflIII
AgeI Aor51HI ApaI AscI AseI
AsnI Asp700I AviII AvrII BalI
BbrPI BcgI BclI BglI BlnI
BsaAI BsaBI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspLU11I BsrDI
BsrGI BssHII BssSI BstBI BstEII
BstPI BstUI ClaI CpoI DraIII
DrdI EaeI EagI EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV FbaI
FseI FspI HpaI KspI MamI
MfeI MluI MluNI MroI MscI
MunI MvnI NdeI NheI NotI
NruI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacII SexAI SfiI SfuI SgrAI
SnaBI SpeI SspBI SspI StuI
SwaI TfiI Van91I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 2788 GGTACC G^GTACC
AcsI 937 AAATTC [GA]^AATT[CT]
AcyI 745 GGCGCC G[GA]^CG[CT]C
AhdI 2170 GACTCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1763 GTGCAC G^TGCAC
ApaLI 1763 GTGCAC G^TGCAC
ApoI 937 AAATTC [GA]^AATT[CT]
Asp718I 2788 GGTACC G^GTACC
AspEI 2170 GACTCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2907 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 745 GGCGCC GGCGC^C
BbvCI 771 GCTGAGG CCTCAGC(-5/-2)
BciVI 876 GTATCC GTATCC(6/5)
BglII 613 AGATCT A^GATCT
BlpI 2673 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2673 GCTAAGC GC^T[ACGT]AGC
BsaHI 745 GGCGCC G[GA]^CG[CT]C
BsaWI 262 ACCGGA [AT]^CCGG[AT]
BsiEI 1451 CGGTCG CG[GA][CT]^CG
BspHI 1954 TCATGA T^CATGA
Bst1107I 1916 GTATAC GTA^TAC
BstZ17I 1916 GTATAC GTA^TAC
CelII 2673 GCTAAGC GC^T[ACGT]AGC
DraI 1857 TTTAAA TTT^AAA
Eam1105I 2170 GACTCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 2796 ATGCAT ATGCA^T
HaeII 745 GGCGCC [GA]GCGC^[CT]
Hin1I 745 GGCGCC G[GA]^CG[CT]C
HincII 1120 GTCGAC GT[CT]^[GA]AC
HindII 1120 GTCGAC GT[CT]^[GA]AC
KasI 745 GGCGCC G^GCGCC
KpnI 2788 GGTACC GGTAC^C
MaeII 1366 ACGT A^CGT
NaeI 604 GCCGGC GCC^GGC
NarI 745 GGCGCC GG^CGCC
NcoI 859 CCATGG C^CATGG
NgoMIV 604 GCCGGC G^CCGGC
NsiI 2796 ATGCAT ATGCA^T
NspI 2798 GCATGC [GA]CATG^[CT]
PflFI 2907 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 1954 TCATGA T^CATGA
SacI 579 GAGCTC GAGCT^C
SalI 1120 GTCGAC G^TCGAC
SapI 654 GAAGAGC GCTCTTC(1/4)
SbfI 104 CCTGCAGG CCTGCA^GG
ScaI 919 AGTACT AGT^ACT
SfoI 745 GGCGCC GGC^GCC
SmaI 2311 CCCGGG CCC^GGG
SphI 2798 GCATGC GCATG^C
Sse8387I 104 CCTGCAGG CCTGCA^GG
Tth111I 2907 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1961 TCTAGA T^CTAGA
XcmI 1621 CCACCTCGCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2311 CCCGGG C^CCGGG
XmaI 2311 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflII 813 CTTAAG C^TTAAG
2521 CTTAAG
AspHI 579 GAGCTC G[AT]GC[AT]^C
1763 GTGCAC
AvaI 975 CCCGAG C^[CT]CG[GA]G
2311 CCCGGG
BamHI 1441 GGATCC G^GATCC
2992 GGATCC
BanI 745 GGCGCC G^G[CT][GA]CC
2788 GGTACC
BanII 513 GAGCCC G[GA]GC[CT]^C
579 GAGCTC
BfrI 813 CTTAAG C^TTAAG
2521 CTTAAG
BsaI 1048 GAGACC GGTCTC(1/5)
2078 GGTCTC
BsgI 636 CTGCAC GTGCAG(16/14)
1465 CTGCAC
BsiHKAI 579 GAGCTC G[AT]GC[AT]^C
1763 GTGCAC
BsmI 1460 GAATGC GAATGC(1/-1)
2878 GCATTC
BsoBI 975 CCCGAG C^[CT]CG[GA]G
2311 CCCGGG
BsrBI 219 GAGCGG CCGCTC(-3/-3)
1543 GAGCGG
BstAPI 184 GCAAAGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
662 GCAGACACTGC
BstXI 880 CCATAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1488 CCAACAGATTGG
DsaI 859 CCATGG C^C[GA][CT]GG
1589 CCGTGG
HgaI 1873 GACGC GACGC(5/10)
1021 GCGTC
HindIII 1790 AAGCTT A^AGCTT
2676 AAGCTT
MspA1I 88 CCGCTG C[AC]G^C[GT]G
200 CCGCTG
PpuMI 15 GGGACCC [GA]G^G[AT]CC[CT]
751 GGGACCT
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AlwNI AocI AvaII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII