##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AspI AviII
AvrII BalI BamHI BbrPI BcgI
BciVI BlnI BsaAI BsaBI BseAI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I ClaI CpoI EaeI EagI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HincII
HindII HindIII HpaI KpnI MamI
MfeI MluI MluNI MroI MscI
MslI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SalI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI SspBI SspI
SwaI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 2488 GTAGAC GT^[AC][GT]AC
AccII 1453 CGCG CG^CG
AflII 18 CTTAAG C^TTAAG
AhdI 1079 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 725 GGATC GGATC(4/5)
Asp700I 310 GAATCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1079 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2250 CTCGGG C^[CT]CG[GA]G
BbeI 1195 GGCGCC GGCGC^C
BclI 658 TGATCA T^GATCA
BfrI 18 CTTAAG C^TTAAG
BglII 338 AGATCT A^GATCT
BlpI 2042 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2042 GCTGAGC GC^T[ACGT]AGC
BsaWI 2184 TCCGGT [AT]^CCGG[AT]
BsmBI 1675 CGTCTC CGTCTC(1/5)
BsoBI 2250 CTCGGG C^[CT]CG[GA]G
BsrDI 160 CATTGC GCAATG(2/0)
BsrFI 970 GCCGGT [GA]^CCGG[CT]
BssSI 2395 CACGAG CACGAG(-5/-1)
BstUI 1453 CGCG CG^CG
BstYI 338 AGATCT [GA]^GATC[CT]
CelII 2042 GCTGAGC GC^T[ACGT]AGC
Cfr10I 970 GCCGGT [GA]^CCGG[CT]
DraI 2444 TTTAAA TTT^AAA
Eam1105I 1079 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 658 TGATCA T^GATCA
KasI 1195 GGCGCC G^GCGCC
KspI 1452 CCGCGG CCGC^GG
MflI 338 AGATCT [GA]^GATC[CT]
MvnI 1453 CGCG CG^CG
NarI 1195 GGCGCC GG^CGCC
PshAI 1316 GACGAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 1452 CCGCGG CCGC^GG
SapI 1655 GAAGAGC GCTCTTC(1/4)
SfoI 1195 GGCGCC GGC^GCC
XhoII 338 AGATCT [GA]^GATC[CT]
XmnI 310 GAATCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 1119 AAATTT [GA]^AATT[CT]
1941 GAATTT
AcyI 872 GACGCC G[GA]^CG[CT]C
1195 GGCGCC
AocI 45 CCTGAGG CC^T[ACGT]AGG
132 CCTGAGG
ApoI 1119 AAATTT [GA]^AATT[CT]
1941 GAATTT
BbvCI 491 CCTCAGC CCTCAGC(-5/-2)
1018 CCTCAGC
BcnI 68 CCGGG CC^[GC]GG
845 CCGGG
BglI 2067 GCCTTTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2233 GCCCCCTGGGC
BsaHI 872 GACGCC G[GA]^CG[CT]C
1195 GGCGCC
BsaI 943 GAGACC GGTCTC(1/5)
2280 GGTCTC
BstAPI 794 GCACTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1805 GCAAATGCTGC
Bsu36I 45 CCTGAGG CC^T[ACGT]AGG
132 CCTGAGG
DraIII 1485 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
1790 CACGCAGTG
DrdI 2215 GACCAGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2272 GACCCTTGGGTC
Eco81I 45 CCTGAGG CC^T[ACGT]AGG
132 CCTGAGG
HgaI 839 GACGC GACGC(5/10)
872 GACGC
Hin1I 872 GACGCC G[GA]^CG[CT]C
1195 GGCGCC
MaeII 900 ACGT A^CGT
1843 ACGT
NciI 68 CCGGG CC^[GC]GG
845 CCGGG
NcoI 497 CCATGG C^CATGG
1849 CCATGG
SacI 1143 GAGCTC GAGCT^C
1248 GAGCTC
SbfI 401 CCTGCAGG CCTGCA^GG
2289 CCTGCAGG
Sse8387I 401 CCTGCAGG CCTGCA^GG
2289 CCTGCAGG
StuI 129 AGGCCT AGG^CCT
2035 AGGCCT
TaqI 1077 TCGA T^CGA
2194 TCGA
TfiI 310 GAATC G^A[AT]TC
990 GATTC
TthHB8I 1077 TCGA T^CGA
2194 TCGA
XbaI 341 TCTAGA T^CTAGA
1094 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AfaI AluI AlwNI ApaI
AspHI AvaII BanI BanII BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrBI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XcmI