##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI AhdI AocI Aor51HI AscI
AseI AsnI Asp718I AspEI AspI
BalI BbeI BbrPI BglI BlpI
Bpu1102I BsaAI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
FseI HaeII HindIII HpaI KasI
KpnI MfeI MluNI MroI MscI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI Sse8387I SspBI SwaI Tth111I
Van91I XcmI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AflII 2162 CTTAAG C^TTAAG
Alw44I 2454 GTGCAC G^TGCAC
AlwNI 158 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 9 GGGCCC GGGCC^C
ApaLI 2454 GTGCAC G^TGCAC
AvaII 1155 GGACC G^G[AT]CC
AviII 2401 TGCGCA TGC^GCA
AvrII 2109 CCTAGG C^CTAGG
BcgI 101 CGATGCGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 704 TGATCA T^GATCA
BfrI 2162 CTTAAG C^TTAAG
BglII 476 AGATCT A^GATCT
BlnI 2109 CCTAGG C^CTAGG
BsaBI 1453 GATGTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 18 ACCGGA [AT]^CCGG[AT]
BsrBI 1921 GAGCGG CCGCTC(-3/-3)
BsrDI 1806 CATTGC GCAATG(2/0)
BssSI 448 CACGAG CACGAG(-5/-1)
CfoI 2402 GCGC GCG^C
EaeI 1287 TGGCCG [CT]^GGCC[GA]
EcoNI 1957 CCTGTAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 238 ATGCAT ATGCA^T
FauI 7 GCGGG CCCGC(4/6)
FbaI 704 TGATCA T^GATCA
FspI 2401 TGCGCA TGC^GCA
HhaI 2402 GCGC GCG^C
HinP1I 2402 GCGC G^CGC
HincII 1124 GTCAAC GT[CT]^[GA]AC
HindII 1124 GTCAAC GT[CT]^[GA]AC
KspI 5 CCGCGG CCGC^GG
MamI 1453 GATGTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 38 ACGCGT A^CGCGT
NcoI 32 CCATGG C^CATGG
NsiI 238 ATGCAT ATGCA^T
PvuII 950 CAGCTG CAG^CTG
SacI 1183 GAGCTC GAGCT^C
SacII 5 CCGCGG CCGC^GG
ScaI 1070 AGTACT AGT^ACT
SmaI 1820 CCCGGG CCC^GGG
SpeI 999 ACTAGT A^CTAGT
SspI 283 AATATT AAT^ATT
StuI 2789 AGGCCT AGG^CCT
XbaI 1848 TCTAGA T^CTAGA
XmaCI 1820 CCCGGG C^CCGGG
XmaI 1820 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 6 CGCG CG^CG
39 CGCG
AcyI 22 GACGCC G[GA]^CG[CT]C
2245 GGCGTC
Asp700I 1864 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2313 GAACCATTTC
BamHI 664 GGATCC G^GATCC
1966 GGATCC
BanI 328 GGTGCC G^G[CT][GA]CC
1350 GGTGCC
BbvCI 26 CCTCAGC CCTCAGC(-5/-2)
2732 GCTGAGG
BciVI 380 GGATAC GTATCC(6/5)
1261 GTATCC
BsaHI 22 GACGCC G[GA]^CG[CT]C
2245 GGCGTC
BsaI 1801 GAGACC GGTCTC(1/5)
2535 GGTCTC
BsgI 1503 CTGCAC GTGCAG(16/14)
2153 GTGCAG
BspLU11I 1396 ACATGT A^CATGT
2546 ACATGT
BstUI 6 CGCG CG^CG
39 CGCG
BstXI 186 CCAAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
279 CCAAAATATTGG
DraI 1246 TTTAAA TTT^AAA
1714 TTTAAA
DraII 1722 AGGCCCT [GA]G^G[ACGT]CC[CT]
2567 AGGGCCT
DsaI 5 CCGCGG C^C[GA][CT]GG
32 CCATGG
EcoO109I 1722 AGGCCCT [GA]G^G[ACGT]CC[CT]
2567 AGGGCCT
Hin1I 22 GACGCC G[GA]^CG[CT]C
2245 GGCGTC
MvnI 6 CGCG CG^CG
39 CGCG
PaeR7I 694 CTCGAG C^TCGAG
1442 CTCGAG
PleI 1122 GAGTC GAGTC(4/5)
2044 GAGTC
SphI 1107 GCATGC GCATG^C
1611 GCATGC
XhoI 694 CTCGAG C^TCGAG
1442 CTCGAG
XmnI 1864 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2313 GAACCATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AspHI AvaI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EarI EcoRI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PstI RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII