##################################
# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspI AviII
AvrII BamHI BbeI BbrPI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BspDI BspEI BspLU11I
BsrBI BssHII Bst1107I BstAPI BstBI
BstUI BstXI BstZ17I CelII ClaI
CpoI DraI DrdI DsaI EagI
EclXI Eco47III Eco52I EcoNI EcoT22I
FauI FbaI FseI FspI HaeII
Hin1I HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI SwaI Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AcsI 1416 GAATTC [GA]^AATT[CT]
AhdI 656 GACCACTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 290 CCTTAGG CC^T[ACGT]AGG
ApoI 1416 GAATTC [GA]^AATT[CT]
Asp700I 1446 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 656 GACCACTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1471 TGGCCA TGG^CCA
BanI 1404 GGCACC G^G[CT][GA]CC
BbsI 1792 GTCTTC GAAGAC(2/6)
BbvCI 2131 CCTCAGC CCTCAGC(-5/-2)
BpmI 1623 CTGGAG CTGGAG(16/14)
BpuAI 1792 GTCTTC GAAGAC(2/6)
BsmBI 1377 CGTCTC CGTCTC(1/5)
Bsp1407I 2052 TGTACA T^GTACA
BsrFI 1285 GCCGGT [GA]^CCGG[CT]
BsrGI 2052 TGTACA T^GTACA
BssSI 913 CACGAG CACGAG(-5/-1)
BstEII 399 GGTCACC G^GT[ACGT]ACC
BstPI 399 GGTCACC G^GT[ACGT]ACC
BstYI 1459 GGATCT [GA]^GATC[CT]
Bsu36I 290 CCTTAGG CC^T[ACGT]AGG
CfoI 2172 GCGC GCG^C
Cfr10I 1285 GCCGGT [GA]^CCGG[CT]
DraIII 80 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 656 GACCACTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 290 CCTTAGG CC^T[ACGT]AGG
EcoO65I 399 GGTCACC G^GT[ACGT]ACC
EcoRI 1416 GAATTC G^AATTC
EcoRV 310 GATATC GAT^ATC
HgaI 535 GCGTC GACGC(5/10)
HhaI 2172 GCGC GCG^C
HinP1I 2172 GCGC G^CGC
HincII 840 GTCAAC GT[CT]^[GA]AC
HindII 840 GTCAAC GT[CT]^[GA]AC
MaeII 935 ACGT A^CGT
MflI 1459 GGATCT [GA]^GATC[CT]
MluNI 1471 TGGCCA TGG^CCA
MscI 1471 TGGCCA TGG^CCA
MslI 1406 CACCCAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1752 CAGCTG C[AC]G^C[GT]G
NspI 2239 GCATGT [GA]CATG^[CT]
PaeR7I 1549 CTCGAG C^TCGAG
PpuMI 193 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 1264 GACTTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1752 CAGCTG CAG^CTG
ScaI 1917 AGTACT AGT^ACT
SspBI 2052 TGTACA T^GTACA
XhoI 1549 CTCGAG C^TCGAG
XhoII 1459 GGATCT [GA]^GATC[CT]
XmnI 1446 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AciI 1427 CCGC CCGC(-3/-1)
1581 GCGG
AlwI 1459 GGATC GGATC(4/5)
1863 GGATC
AlwNI 247 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
721 CAGTGGCTG
AvaI 712 CCCGAG C^[CT]CG[GA]G
1549 CTCGAG
BsoBI 712 CCCGAG C^[CT]CG[GA]G
1549 CTCGAG
BsrDI 2235 CATTGC GCAATG(2/0)
2271 GCAATG
Cac8I 1618 GCTGGC GC[ACGT]^[ACGT]GC
2086 GCTGGC
DpnII 1460 GATC ^GATC
1864 GATC
DraII 10 AGGGCCT [GA]G^G[ACGT]CC[CT]
193 GGGACCC
EcoO109I 10 AGGGCCT [GA]G^G[ACGT]CC[CT]
193 GGGACCC
HapII 1286 CCGG C^CGG
2091 CCGG
HpaII 1286 CCGG C^CGG
2091 CCGG
MboI 1460 GATC ^GATC
1864 GATC
MspI 1286 CCGG C^CGG
2091 CCGG
NdeI 55 CATATG CA^TATG
1140 CATATG
NdeII 1460 GATC ^GATC
1864 GATC
Sau3AI 1460 GATC ^GATC
1864 GATC
SfaNI 227 GCATC GCATC(5/9)
2174 GCATC
SspI 1039 AATATT AAT^ATT
1949 AATATT
XbaI 1245 TCTAGA T^CTAGA
2204 TCTAGA
XcmI 1480 CCAGAGAGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1744 CCAAACTGCAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AfaI AluI AspHI AvaII BanII
BbvI BfaI BmyI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspHI BspMI
BsrI BssKI BstF5I BstNI Cfr13I
DdeI EaeI EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HindIII HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboII MnlI MseI MvaI MwoI
NlaIII NlaIV PflMI PleI PstI
RcaI RsaI SacI Sau96I ScrFI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I