##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
Acc65I AccII AclI AflII AgeI
AhdI AocI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BbeI BbrPI BbvCI BciVI
BclI BfrI BlnI BlpI Bpu1102I
BsaAI BsaBI BsiWI BsrBI BsrDI
BssHII BssSI BstAPI BstBI BstUI
Bsu36I CelII CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI KasI KpnI
KspI MamI MfeI MluI MunI
MvnI NarI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I RsrII
SacII SalI SapI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI SspI SwaI Van91I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 2482 GACGTC GACGT^C
AccI 1165 GTATAC GT^[AC][GT]AC
AcyI 2482 GACGTC G[GA]^CG[CT]C
BfaI 1250 CTAG C^TAG
BglII 1185 AGATCT A^GATCT
BsaHI 2482 GACGTC G[GA]^CG[CT]C
BsiEI 2184 CGATCG CG[GA][CT]^CG
BsmI 1102 GAATGC GAATGC(1/-1)
Bsp1407I 525 TGTACA T^GTACA
BspDI 2186 ATCGAT AT^CGAT
BsrGI 525 TGTACA T^GTACA
Bst1107I 1165 GTATAC GTA^TAC
BstEII 836 GGTGACC G^GT[ACGT]ACC
BstPI 836 GGTGACC G^GT[ACGT]ACC
BstXI 1206 CCAACGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1165 GTATAC GTA^TAC
ClaI 2186 ATCGAT AT^CGAT
DraIII 2724 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 726 CCGTGG C^C[GA][CT]GG
EcoO65I 836 GGTGACC G^GT[ACGT]ACC
EcoT14I 1794 CCTTGG C^C[AT][AT]GG
Hin1I 2482 GACGTC G[GA]^CG[CT]C
HindIII 350 AAGCTT A^AGCTT
HpaI 39 GTTAAC GTT^AAC
MaeI 1250 CTAG C^TAG
PvuI 2184 CGATCG CGAT^CG
SacI 1001 GAGCTC GAGCT^C
SbfI 2562 CCTGCAGG CCTGCA^GG
ScaI 49 AGTACT AGT^ACT
SmaI 622 CCCGGG CCC^GGG
SmlI 2701 CTCAAG C^T[CT][GA]AG
Sse8387I 2562 CCTGCAGG CCTGCA^GG
SspBI 525 TGTACA T^GTACA
StyI 1794 CCTTGG C^C[AT][AT]GG
XmaCI 622 CCCGGG C^CCGGG
XmaI 622 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccIII 1635 TCCGGA T^CCGGA
1849 TCCGGA
AflIII 2112 ACATGT A^C[GA][CT]GT
2508 ACATGT
Alw44I 1374 GTGCAC G^TGCAC
1612 GTGCAC
ApaLI 1374 GTGCAC G^TGCAC
1612 GTGCAC
Asp700I 1820 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2616 GAATCTCTTC
AvaI 622 CCCGGG C^[CT]CG[GA]G
1840 CTCGGG
BalI 954 TGGCCA TGG^CCA
1294 TGGCCA
BamHI 1638 GGATCC G^GATCC
2094 GGATCC
BcgI 1096 CGAAGAGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2178 GCAAAACGATCG
BglI 663 GCCCATACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
813 GCCTGACAGGC
BsaI 977 GAGACC GGTCTC(1/5)
1835 GAGACC
BsaWI 1635 TCCGGA [AT]^CCGG[AT]
1849 TCCGGA
BseAI 1635 TCCGGA T^CCGGA
1849 TCCGGA
BseRI 1270 CTCCTC GAGGAG(10/8)
2541 CTCCTC
BsoBI 622 CCCGGG C^[CT]CG[GA]G
1840 CTCGGG
BspEI 1635 TCCGGA T^CCGGA
1849 TCCGGA
BspHI 879 TCATGA T^CATGA
1147 TCATGA
BspLU11I 2112 ACATGT A^CATGT
2508 ACATGT
BsrFI 1655 GCCGGC [GA]^CCGG[CT]
2338 GCCGGC
CfoI 451 GCGC GCG^C
2599 GCGC
Cfr10I 1655 GCCGGC [GA]^CCGG[CT]
2338 GCCGGC
EarI 2620 CTCTTC CTCTTC(1/4)
1097 GAAGAG
EcoNI 1128 CCTTCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2439 CCTCCAAGAGG
HhaI 451 GCGC GCG^C
2599 GCGC
HinP1I 451 GCGC G^CGC
2599 GCGC
Ksp632I 2620 CTCTTC CTCTTC(1/4)
1097 GAAGAG
MluNI 954 TGGCCA TGG^CCA
1294 TGGCCA
MroI 1635 TCCGGA T^CCGGA
1849 TCCGGA
MscI 954 TGGCCA TGG^CCA
1294 TGGCCA
MslI 398 CATCCGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2503 CATGGACATG
NaeI 1655 GCCGGC GCC^GGC
2338 GCCGGC
NgoMIV 1655 GCCGGC G^CCGGC
2338 GCCGGC
PstI 1894 CTGCAG CTGCA^G
2563 CTGCAG
RcaI 879 TCATGA T^CATGA
1147 TCATGA
SfcI 1894 CTGCAG C^T[GA][CT]AG
2563 CTGCAG
StuI 1380 AGGCCT AGG^CCT
2567 AGGCCT
XcmI 267 CCATAACAGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1035 CCAATACTCCTGTGG
XmnI 1820 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2616 GAATCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AspI AvaII
BanI BanII BbsI BbvI BcnI
BmyI BpmI BpuAI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRI EcoRII FauI Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflFI
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XhoII