################################## # NO CUT ENZYMES ( 96 enzymes) # ################################## Acc65I AccII AclI AflII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AviII AvrII BbeI BbrPI BbvCI BciVI BclI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BsiWI BsrBI BsrDI BssHII BssSI BstAPI BstBI BstUI Bsu36I CelII CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRV EcoT22I FbaI FseI FspI HaeII HgaI KasI KpnI KspI MamI MfeI MluI MunI MvnI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I RsrII SacII SalI SapI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI SspI SwaI Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AatII 2482 GACGTC GACGT^C AccI 1165 GTATAC GT^[AC][GT]AC AcyI 2482 GACGTC G[GA]^CG[CT]C BfaI 1250 CTAG C^TAG BglII 1185 AGATCT A^GATCT BsaHI 2482 GACGTC G[GA]^CG[CT]C BsiEI 2184 CGATCG CG[GA][CT]^CG BsmI 1102 GAATGC GAATGC(1/-1) Bsp1407I 525 TGTACA T^GTACA BspDI 2186 ATCGAT AT^CGAT BsrGI 525 TGTACA T^GTACA Bst1107I 1165 GTATAC GTA^TAC BstEII 836 GGTGACC G^GT[ACGT]ACC BstPI 836 GGTGACC G^GT[ACGT]ACC BstXI 1206 CCAACGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 1165 GTATAC GTA^TAC ClaI 2186 ATCGAT AT^CGAT DraIII 2724 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 726 CCGTGG C^C[GA][CT]GG EcoO65I 836 GGTGACC G^GT[ACGT]ACC EcoT14I 1794 CCTTGG C^C[AT][AT]GG Hin1I 2482 GACGTC G[GA]^CG[CT]C HindIII 350 AAGCTT A^AGCTT HpaI 39 GTTAAC GTT^AAC MaeI 1250 CTAG C^TAG PvuI 2184 CGATCG CGAT^CG SacI 1001 GAGCTC GAGCT^C SbfI 2562 CCTGCAGG CCTGCA^GG ScaI 49 AGTACT AGT^ACT SmaI 622 CCCGGG CCC^GGG SmlI 2701 CTCAAG C^T[CT][GA]AG Sse8387I 2562 CCTGCAGG CCTGCA^GG SspBI 525 TGTACA T^GTACA StyI 1794 CCTTGG C^C[AT][AT]GG XmaCI 622 CCCGGG C^CCGGG XmaI 622 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 38 enzymes) # ################################### AccIII 1635 TCCGGA T^CCGGA 1849 TCCGGA AflIII 2112 ACATGT A^C[GA][CT]GT 2508 ACATGT Alw44I 1374 GTGCAC G^TGCAC 1612 GTGCAC ApaLI 1374 GTGCAC G^TGCAC 1612 GTGCAC Asp700I 1820 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2616 GAATCTCTTC AvaI 622 CCCGGG C^[CT]CG[GA]G 1840 CTCGGG BalI 954 TGGCCA TGG^CCA 1294 TGGCCA BamHI 1638 GGATCC G^GATCC 2094 GGATCC BcgI 1096 CGAAGAGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2178 GCAAAACGATCG BglI 663 GCCCATACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 813 GCCTGACAGGC BsaI 977 GAGACC GGTCTC(1/5) 1835 GAGACC BsaWI 1635 TCCGGA [AT]^CCGG[AT] 1849 TCCGGA BseAI 1635 TCCGGA T^CCGGA 1849 TCCGGA BseRI 1270 CTCCTC GAGGAG(10/8) 2541 CTCCTC BsoBI 622 CCCGGG C^[CT]CG[GA]G 1840 CTCGGG BspEI 1635 TCCGGA T^CCGGA 1849 TCCGGA BspHI 879 TCATGA T^CATGA 1147 TCATGA BspLU11I 2112 ACATGT A^CATGT 2508 ACATGT BsrFI 1655 GCCGGC [GA]^CCGG[CT] 2338 GCCGGC CfoI 451 GCGC GCG^C 2599 GCGC Cfr10I 1655 GCCGGC [GA]^CCGG[CT] 2338 GCCGGC EarI 2620 CTCTTC CTCTTC(1/4) 1097 GAAGAG EcoNI 1128 CCTTCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2439 CCTCCAAGAGG HhaI 451 GCGC GCG^C 2599 GCGC HinP1I 451 GCGC G^CGC 2599 GCGC Ksp632I 2620 CTCTTC CTCTTC(1/4) 1097 GAAGAG MluNI 954 TGGCCA TGG^CCA 1294 TGGCCA MroI 1635 TCCGGA T^CCGGA 1849 TCCGGA MscI 954 TGGCCA TGG^CCA 1294 TGGCCA MslI 398 CATCCGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2503 CATGGACATG NaeI 1655 GCCGGC GCC^GGC 2338 GCCGGC NgoMIV 1655 GCCGGC G^CCGGC 2338 GCCGGC PstI 1894 CTGCAG CTGCA^G 2563 CTGCAG RcaI 879 TCATGA T^CATGA 1147 TCATGA SfcI 1894 CTGCAG C^T[GA][CT]AG 2563 CTGCAG StuI 1380 AGGCCT AGG^CCT 2567 AGGCCT XcmI 267 CCATAACAGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1035 CCAATACTCCTGTGG XmnI 1820 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2616 GAATCTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 88 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AspI AvaII BanI BanII BbsI BbvI BcnI BmyI BpmI BpuAI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRI EcoRII FauI Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflFI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XhoII