################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## AclI AflII AflIII AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp700I AspEI AviII BbrPI BciVI BclI BfrI BglII BlpI Bpu1102I BsaAI BsaBI BsiEI BsiWI BsmI BspDI BspHI BspLU11I BssHII BstAPI BstBI CelII ClaI CpoI DraI EagI Eam1105I EclXI Eco52I EcoRI EcoRV EcoT22I FbaI FseI FspI HpaI KspI MamI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI SexAI SfiI SfuI SgrAI SnaBI Sse8387I SspI SwaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 2584 GACGTC GACGT^C Acc65I 2186 GGTACC G^GTACC AccIII 2313 TCCGGA T^CCGGA AcsI 1993 AAATTC [GA]^AATT[CT] Aor51HI 903 AGCGCT AGC^GCT ApaI 1245 GGGCCC GGGCC^C ApoI 1993 AAATTC [GA]^AATT[CT] Asp718I 2186 GGTACC G^GTACC AspI 2581 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 3017 CCTAGG C^CTAGG BcgI 2421 GCACCATGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlnI 3017 CCTAGG C^CTAGG BseAI 2313 TCCGGA T^CCGGA BsgI 991 CTGCAC GTGCAG(16/14) BsmBI 2586 CGTCTC CGTCTC(1/5) Bsp1407I 1122 TGTACA T^GTACA BspEI 2313 TCCGGA T^CCGGA BsrBI 1220 GAGCGG CCGCTC(-3/-3) BsrDI 1584 CATTGC GCAATG(2/0) BsrGI 1122 TGTACA T^GTACA Bst1107I 824 GTATAC GTA^TAC BstEII 2939 GGTCACC G^GT[ACGT]ACC BstPI 2939 GGTCACC G^GT[ACGT]ACC BstZ17I 824 GTATAC GTA^TAC DraIII 3035 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 903 AGCGCT AGC^GCT EcoNI 2221 CCTCCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 2939 GGTCACC G^GT[ACGT]ACC HgaI 1793 GCGTC GACGC(5/10) HincII 430 GTCAAC GT[CT]^[GA]AC HindII 430 GTCAAC GT[CT]^[GA]AC HindIII 2053 AAGCTT A^AGCTT KpnI 2186 GGTACC GGTAC^C MroI 2313 TCCGGA T^CCGGA MseI 3071 TTAA T^TAA NcoI 2424 CCATGG C^CATGG PflFI 2581 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 1928 GACCGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 781 GAAGAGC GCTCTTC(1/4) ScaI 177 AGTACT AGT^ACT SmlI 1802 CTCAAG C^T[CT][GA]AG SpeI 2134 ACTAGT A^CTAGT SphI 801 GCATGC GCATG^C SspBI 1122 TGTACA T^GTACA Tru9I 3071 TTAA T^TAA Tth111I 2581 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccI 752 GTCTAC GT^[AC][GT]AC 824 GTATAC AccII 695 CGCG CG^CG 1157 CGCG BamHI 1954 GGATCC G^GATCC 2851 GGATCC BbsI 100 GAAGAC GAAGAC(2/6) 958 GAAGAC BpuAI 100 GAAGAC GAAGAC(2/6) 958 GAAGAC BsaI 2375 GAGACC GGTCTC(1/5) 2467 GGTCTC BstUI 695 CGCG CG^CG 1157 CGCG BstXI 769 CCACCTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2953 CCAGCTCTGTGG DrdI 743 GACTGCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2639 GACAAGTGAGTC EarI 188 CTCTTC CTCTTC(1/4) 781 GAAGAG Ksp632I 188 CTCTTC CTCTTC(1/4) 781 GAAGAG MvnI 695 CGCG CG^CG 1157 CGCG PpuMI 1906 AGGTCCC [GA]G^G[AT]CC[CT] 2889 GGGTCCC SmaI 197 CCCGGG CCC^GGG 2618 CCCGGG TaqI 1023 TCGA T^CGA 2049 TCGA Tsp509I 1994 AATT ^AATT 3154 AATT TthHB8I 1023 TCGA T^CGA 2049 TCGA XbaI 1936 TCTAGA T^CTAGA 2590 TCTAGA XmaCI 197 CCCGGG C^CCGGG 2618 CCCGGG XmaI 197 CCCGGG C^CCGGG 2618 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 110 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AlwNI AocI AspHI AvaI AvaII BalI BanI BanII BbeI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BsaHI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BssSI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI StuI StyI TfiI TseI Tsp45I TspRI Van91I XcmI XhoII