##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AclI AflII AflIII AgeI AhdI
Alw44I ApaLI AscI AseI AsnI
Asp700I AspEI AviII BbrPI BciVI
BclI BfrI BglII BlpI Bpu1102I
BsaAI BsaBI BsiEI BsiWI BsmI
BspDI BspHI BspLU11I BssHII BstAPI
BstBI CelII ClaI CpoI DraI
EagI Eam1105I EclXI Eco52I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HpaI KspI MamI MfeI MluI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI SexAI SfiI SfuI
SgrAI SnaBI Sse8387I SspI SwaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 2584 GACGTC GACGT^C
Acc65I 2186 GGTACC G^GTACC
AccIII 2313 TCCGGA T^CCGGA
AcsI 1993 AAATTC [GA]^AATT[CT]
Aor51HI 903 AGCGCT AGC^GCT
ApaI 1245 GGGCCC GGGCC^C
ApoI 1993 AAATTC [GA]^AATT[CT]
Asp718I 2186 GGTACC G^GTACC
AspI 2581 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3017 CCTAGG C^CTAGG
BcgI 2421 GCACCATGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 3017 CCTAGG C^CTAGG
BseAI 2313 TCCGGA T^CCGGA
BsgI 991 CTGCAC GTGCAG(16/14)
BsmBI 2586 CGTCTC CGTCTC(1/5)
Bsp1407I 1122 TGTACA T^GTACA
BspEI 2313 TCCGGA T^CCGGA
BsrBI 1220 GAGCGG CCGCTC(-3/-3)
BsrDI 1584 CATTGC GCAATG(2/0)
BsrGI 1122 TGTACA T^GTACA
Bst1107I 824 GTATAC GTA^TAC
BstEII 2939 GGTCACC G^GT[ACGT]ACC
BstPI 2939 GGTCACC G^GT[ACGT]ACC
BstZ17I 824 GTATAC GTA^TAC
DraIII 3035 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 903 AGCGCT AGC^GCT
EcoNI 2221 CCTCCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2939 GGTCACC G^GT[ACGT]ACC
HgaI 1793 GCGTC GACGC(5/10)
HincII 430 GTCAAC GT[CT]^[GA]AC
HindII 430 GTCAAC GT[CT]^[GA]AC
HindIII 2053 AAGCTT A^AGCTT
KpnI 2186 GGTACC GGTAC^C
MroI 2313 TCCGGA T^CCGGA
MseI 3071 TTAA T^TAA
NcoI 2424 CCATGG C^CATGG
PflFI 2581 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1928 GACCGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 781 GAAGAGC GCTCTTC(1/4)
ScaI 177 AGTACT AGT^ACT
SmlI 1802 CTCAAG C^T[CT][GA]AG
SpeI 2134 ACTAGT A^CTAGT
SphI 801 GCATGC GCATG^C
SspBI 1122 TGTACA T^GTACA
Tru9I 3071 TTAA T^TAA
Tth111I 2581 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 752 GTCTAC GT^[AC][GT]AC
824 GTATAC
AccII 695 CGCG CG^CG
1157 CGCG
BamHI 1954 GGATCC G^GATCC
2851 GGATCC
BbsI 100 GAAGAC GAAGAC(2/6)
958 GAAGAC
BpuAI 100 GAAGAC GAAGAC(2/6)
958 GAAGAC
BsaI 2375 GAGACC GGTCTC(1/5)
2467 GGTCTC
BstUI 695 CGCG CG^CG
1157 CGCG
BstXI 769 CCACCTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2953 CCAGCTCTGTGG
DrdI 743 GACTGCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2639 GACAAGTGAGTC
EarI 188 CTCTTC CTCTTC(1/4)
781 GAAGAG
Ksp632I 188 CTCTTC CTCTTC(1/4)
781 GAAGAG
MvnI 695 CGCG CG^CG
1157 CGCG
PpuMI 1906 AGGTCCC [GA]G^G[AT]CC[CT]
2889 GGGTCCC
SmaI 197 CCCGGG CCC^GGG
2618 CCCGGG
TaqI 1023 TCGA T^CGA
2049 TCGA
Tsp509I 1994 AATT ^AATT
3154 AATT
TthHB8I 1023 TCGA T^CGA
2049 TCGA
XbaI 1936 TCTAGA T^CTAGA
2590 TCTAGA
XmaCI 197 CCCGGG C^CCGGG
2618 CCCGGG
XmaI 197 CCCGGG C^CCGGG
2618 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AocI AspHI AvaI AvaII
BalI BanI BanII BbeI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BsaHI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BssSI BstF5I BstNI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI StuI StyI TfiI TseI
Tsp45I TspRI Van91I XcmI XhoII