################################## # NO CUT ENZYMES ( 111 enzymes) # ################################## AclI AflII AflIII AgeI AhdI Alw44I AocI ApaLI AscI AseI AsnI AspEI AspI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BcgI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsiEI BsiWI BsmBI BsmFI BspLU11I BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstPI BstXI Bsu36I CelII Cfr10I CpoI DraIII EaeI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoO65I EcoRI EcoT22I FseI FspI HincII HindII HpaI Ksp632I KspI MamI MfeI MluI MluNI MscI MunI NaeI NgoMIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AatII 1455 GACGTC GACGT^C Acc65I 2664 GGTACC G^GTACC AccI 1598 GTATAC GT^[AC][GT]AC AccII 36 CGCG CG^CG AccIII 649 TCCGGA T^CCGGA AlwI 693 GATCC GGATC(4/5) ApaI 117 GGGCCC GGGCC^C Asp718I 2664 GGTACC G^GTACC AspHI 2456 GAGCTC G[AT]GC[AT]^C AvaII 2192 GGACC G^G[AT]CC BbeI 459 GGCGCC GGCGC^C BciVI 2147 GGATAC GTATCC(6/5) BpmI 399 CTGGAG CTGGAG(16/14) BseAI 649 TCCGGA T^CCGGA BseRI 1107 GAGGAG GAGGAG(10/8) BsgI 2421 GTGCAG GTGCAG(16/14) BsiHKAI 2456 GAGCTC G[AT]GC[AT]^C BsmI 1480 GAATGC GAATGC(1/-1) BspDI 2064 ATCGAT AT^CGAT BspEI 649 TCCGGA T^CCGGA BsrDI 2436 CATTGC GCAATG(2/0) Bst1107I 1598 GTATAC GTA^TAC BstUI 36 CGCG CG^CG BstYI 692 AGATCC [GA]^GATC[CT] BstZ17I 1598 GTATAC GTA^TAC ClaI 2064 ATCGAT AT^CGAT DraI 176 TTTAAA TTT^AAA DsaI 713 CCATGG C^C[GA][CT]GG EcoNI 1157 CCTTACTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 713 CCATGG C^C[AT][AT]GG FauI 98 GCGGG CCCGC(4/6) HgaI 2556 GCGTC GACGC(5/10) HindIII 835 AAGCTT A^AGCTT KasI 459 GGCGCC G^GCGCC KpnI 2664 GGTACC GGTAC^C MflI 692 AGATCC [GA]^GATC[CT] MroI 649 TCCGGA T^CCGGA MvnI 36 CGCG CG^CG NarI 459 GGCGCC GG^CGCC NcoI 713 CCATGG C^CATGG PflMI 2488 CCATACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 402 GAGTC GAGTC(4/5) PvuII 2424 CAGCTG CAG^CTG SacI 2456 GAGCTC GAGCT^C ScaI 1348 AGTACT AGT^ACT SfoI 459 GGCGCC GGC^GCC StuI 2476 AGGCCT AGG^CCT StyI 713 CCATGG C^C[AT][AT]GG Van91I 2488 CCATACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1805 CCAGCATTTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 692 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcyI 459 GGCGCC G[GA]^CG[CT]C 1455 GACGTC AlwNI 526 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG 2507 CAGCCCCTG Aor51HI 17 AGCGCT AGC^GCT 1617 AGCGCT Asp700I 627 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 879 GAAAAGGTTC AvaI 74 CCCGGG C^[CT]CG[GA]G 114 CCCGGG BanI 459 GGCGCC G^G[CT][GA]CC 2664 GGTACC BclI 1250 TGATCA T^GATCA 1540 TGATCA BsaHI 459 GGCGCC G[GA]^CG[CT]C 1455 GACGTC BsaWI 649 TCCGGA [AT]^CCGG[AT] 2194 ACCGGA BsmAI 204 GAGAC GTCTC(1/5) 794 GTCTC BsoBI 74 CCCGGG C^[CT]CG[GA]G 114 CCCGGG Bsp1407I 1778 TGTACA T^GTACA 1848 TGTACA BsrGI 1778 TGTACA T^GTACA 1848 TGTACA BstNI 2296 CCAGG CC^[AT]GG 2417 CCTGG DrdI 1449 GACAAGGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1605 GACATGAGTGTC Eco47III 17 AGCGCT AGC^GCT 1617 AGCGCT EcoRII 2296 CCAGG ^CC[AT]GG 2417 CCTGG EcoRV 291 GATATC GAT^ATC 2061 GATATC FbaI 1250 TGATCA T^GATCA 1540 TGATCA Hin1I 459 GGCGCC G[GA]^CG[CT]C 1455 GACGTC MspA1I 972 CAGCGG C[AC]G^C[GT]G 2424 CAGCTG MvaI 2296 CCAGG CC^[AT]GG 2417 CCTGG NheI 593 GCTAGC G^CTAGC 1484 GCTAGC SmaI 74 CCCGGG CCC^GGG 114 CCCGGG SspBI 1778 TGTACA T^GTACA 1848 TGTACA Tsp45I 370 GTCAC ^GT[GC]AC 1354 GTGAC XmaCI 74 CCCGGG C^CCGGG 114 CCCGGG XmaI 74 CCCGGG C^CCGGG 114 CCCGGG XmnI 627 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 879 GAAAAGGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI BanII BbvI BcnI BfaI BmyI BsaJI BsiYI BslI Bsp1286I BspHI BsrI BssKI BstF5I Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspI MwoI NciI NdeI NdeII NlaIII NlaIV PstI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I