##################################
# NO CUT ENZYMES ( 111 enzymes) #
##################################
AclI AflII AflIII AgeI AhdI
Alw44I AocI ApaLI AscI AseI
AsnI AspEI AspI AviII AvrII
BalI BamHI BbrPI BbsI BbvCI
BcgI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsiEI BsiWI BsmBI BsmFI
BspLU11I BspMI BsrBI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstPI
BstXI Bsu36I CelII Cfr10I CpoI
DraIII EaeI EagI Eam1105I EarI
EclXI Eco52I Eco81I EcoO65I EcoRI
EcoT22I FseI FspI HincII HindII
HpaI Ksp632I KspI MamI MfeI
MluI MluNI MscI MunI NaeI
NgoMIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SwaI Tth111I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 1455 GACGTC GACGT^C
Acc65I 2664 GGTACC G^GTACC
AccI 1598 GTATAC GT^[AC][GT]AC
AccII 36 CGCG CG^CG
AccIII 649 TCCGGA T^CCGGA
AlwI 693 GATCC GGATC(4/5)
ApaI 117 GGGCCC GGGCC^C
Asp718I 2664 GGTACC G^GTACC
AspHI 2456 GAGCTC G[AT]GC[AT]^C
AvaII 2192 GGACC G^G[AT]CC
BbeI 459 GGCGCC GGCGC^C
BciVI 2147 GGATAC GTATCC(6/5)
BpmI 399 CTGGAG CTGGAG(16/14)
BseAI 649 TCCGGA T^CCGGA
BseRI 1107 GAGGAG GAGGAG(10/8)
BsgI 2421 GTGCAG GTGCAG(16/14)
BsiHKAI 2456 GAGCTC G[AT]GC[AT]^C
BsmI 1480 GAATGC GAATGC(1/-1)
BspDI 2064 ATCGAT AT^CGAT
BspEI 649 TCCGGA T^CCGGA
BsrDI 2436 CATTGC GCAATG(2/0)
Bst1107I 1598 GTATAC GTA^TAC
BstUI 36 CGCG CG^CG
BstYI 692 AGATCC [GA]^GATC[CT]
BstZ17I 1598 GTATAC GTA^TAC
ClaI 2064 ATCGAT AT^CGAT
DraI 176 TTTAAA TTT^AAA
DsaI 713 CCATGG C^C[GA][CT]GG
EcoNI 1157 CCTTACTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 713 CCATGG C^C[AT][AT]GG
FauI 98 GCGGG CCCGC(4/6)
HgaI 2556 GCGTC GACGC(5/10)
HindIII 835 AAGCTT A^AGCTT
KasI 459 GGCGCC G^GCGCC
KpnI 2664 GGTACC GGTAC^C
MflI 692 AGATCC [GA]^GATC[CT]
MroI 649 TCCGGA T^CCGGA
MvnI 36 CGCG CG^CG
NarI 459 GGCGCC GG^CGCC
NcoI 713 CCATGG C^CATGG
PflMI 2488 CCATACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 402 GAGTC GAGTC(4/5)
PvuII 2424 CAGCTG CAG^CTG
SacI 2456 GAGCTC GAGCT^C
ScaI 1348 AGTACT AGT^ACT
SfoI 459 GGCGCC GGC^GCC
StuI 2476 AGGCCT AGG^CCT
StyI 713 CCATGG C^C[AT][AT]GG
Van91I 2488 CCATACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1805 CCAGCATTTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 692 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 459 GGCGCC G[GA]^CG[CT]C
1455 GACGTC
AlwNI 526 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2507 CAGCCCCTG
Aor51HI 17 AGCGCT AGC^GCT
1617 AGCGCT
Asp700I 627 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
879 GAAAAGGTTC
AvaI 74 CCCGGG C^[CT]CG[GA]G
114 CCCGGG
BanI 459 GGCGCC G^G[CT][GA]CC
2664 GGTACC
BclI 1250 TGATCA T^GATCA
1540 TGATCA
BsaHI 459 GGCGCC G[GA]^CG[CT]C
1455 GACGTC
BsaWI 649 TCCGGA [AT]^CCGG[AT]
2194 ACCGGA
BsmAI 204 GAGAC GTCTC(1/5)
794 GTCTC
BsoBI 74 CCCGGG C^[CT]CG[GA]G
114 CCCGGG
Bsp1407I 1778 TGTACA T^GTACA
1848 TGTACA
BsrGI 1778 TGTACA T^GTACA
1848 TGTACA
BstNI 2296 CCAGG CC^[AT]GG
2417 CCTGG
DrdI 1449 GACAAGGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1605 GACATGAGTGTC
Eco47III 17 AGCGCT AGC^GCT
1617 AGCGCT
EcoRII 2296 CCAGG ^CC[AT]GG
2417 CCTGG
EcoRV 291 GATATC GAT^ATC
2061 GATATC
FbaI 1250 TGATCA T^GATCA
1540 TGATCA
Hin1I 459 GGCGCC G[GA]^CG[CT]C
1455 GACGTC
MspA1I 972 CAGCGG C[AC]G^C[GT]G
2424 CAGCTG
MvaI 2296 CCAGG CC^[AT]GG
2417 CCTGG
NheI 593 GCTAGC G^CTAGC
1484 GCTAGC
SmaI 74 CCCGGG CCC^GGG
114 CCCGGG
SspBI 1778 TGTACA T^GTACA
1848 TGTACA
Tsp45I 370 GTCAC ^GT[GC]AC
1354 GTGAC
XmaCI 74 CCCGGG C^CCGGG
114 CCCGGG
XmaI 74 CCCGGG C^CCGGG
114 CCCGGG
XmnI 627 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
879 GAAAAGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BanII BbvI BcnI BfaI BmyI
BsaJI BsiYI BslI Bsp1286I BspHI
BsrI BssKI BstF5I Cac8I CfoI
Cfr13I DdeI DpnII DraII EcoO109I
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspI
MwoI NciI NdeI NdeII NlaIII
NlaIV PstI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I