##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
Aor51HI AscI Asp700I AspI AviII
BamHI BbrPI BbvCI BcgI BciVI
BclI BfrI BglII BsaAI BseAI
BsiWI BspDI BspEI BssHII Bst1107I
BstAPI BstZ17I ClaI CpoI DraI
DraIII DrdI Eco47III EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HpaI KspI MaeII MfeI
MluI MroI MunI NaeI NdeI
NgoMIV NsiI PacI PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacII SalI ScaI
SgrAI SnaBI SpeI SphI SspI
SwaI Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
Acc65I 1249 GGTACC G^GTACC
AccI 483 GTCTAC GT^[AC][GT]AC
AcsI 2213 AAATTC [GA]^AATT[CT]
AhdI 1553 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1199 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1759 GGGCCC GGGCC^C
ApoI 2213 AAATTC [GA]^AATT[CT]
AseI 2243 ATTAAT AT^TAAT
AsnI 2243 ATTAAT AT^TAAT
Asp718I 1249 GGTACC G^GTACC
AspEI 1553 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2116 CCTAGG C^CTAGG
BalI 2752 TGGCCA TGG^CCA
BbeI 810 GGCGCC GGCGC^C
BlnI 2116 CCTAGG C^CTAGG
BsaBI 573 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1246 TCCGGT [AT]^CCGG[AT]
BsmBI 22 GAGACG CGTCTC(1/5)
BsmI 500 GCATTC GAATGC(1/-1)
BspMI 2568 GCAGGT ACCTGC(4/8)
BsrDI 254 GCAATG GCAATG(2/0)
BstBI 1780 TTCGAA TT^CGAA
BstEII 723 GGTGACC G^GT[ACGT]ACC
BstPI 723 GGTGACC G^GT[ACGT]ACC
Eam1105I 1553 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1674 CCTGCGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 723 GGTGACC G^GT[ACGT]ACC
HindIII 4 AAGCTT A^AGCTT
KasI 810 GGCGCC G^GCGCC
KpnI 1249 GGTACC GGTAC^C
MamI 573 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2752 TGGCCA TGG^CCA
MscI 2752 TGGCCA TGG^CCA
NarI 810 GGCGCC GG^CGCC
NheI 58 GCTAGC G^CTAGC
NotI 1690 GCGGCCGC GC^GGCCGC
NruI 1645 TCGCGA TCG^CGA
NspV 1780 TTCGAA TT^CGAA
PaeR7I 119 CTCGAG C^TCGAG
PflMI 2599 CCAACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 2243 ATTAAT AT^TAAT
PvuII 1798 CAGCTG CAG^CTG
SapI 991 GAAGAGC GCTCTTC(1/4)
SbfI 2565 CCTGCAGG CCTGCA^GG
SexAI 41 ACCTGGT A^CC[AT]GGT
SfiI 1683 GGCCACAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 810 GGCGCC GGC^GCC
SfuI 1780 TTCGAA TT^CGAA
SmaI 1946 CCCGGG CCC^GGG
Sse8387I 2565 CCTGCAGG CCTGCA^GG
StuI 695 AGGCCT AGG^CCT
Van91I 2599 CCAACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1280 CCAGTCATAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 119 CTCGAG C^TCGAG
XmaCI 1946 CCCGGG C^CCGGG
XmaI 1946 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 810 GGCGCC G[GA]^CG[CT]C
1822 GGCGTC
AflIII 479 ACATGT A^C[GA][CT]GT
2814 ACATGT
Alw44I 151 GTGCAC G^TGCAC
1185 GTGCAC
ApaLI 151 GTGCAC G^TGCAC
1185 GTGCAC
BbsI 30 GAAGAC GAAGAC(2/6)
753 GAAGAC
BpuAI 30 GAAGAC GAAGAC(2/6)
753 GAAGAC
BsaHI 810 GGCGCC G[GA]^CG[CT]C
1822 GGCGTC
BsaI 1370 GAGACC GGTCTC(1/5)
2403 GGTCTC
BsiEI 96 CGGCCG CG[GA][CT]^CG
1691 CGGCCG
BspHI 557 TCATGA T^CATGA
2020 TCATGA
BspLU11I 479 ACATGT A^CATGT
2814 ACATGT
BsrFI 1373 ACCGGC [GA]^CCGG[CT]
1703 GCCGGT
BstXI 1061 CCAGCCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2577 CCAGGGAGCTGG
Cfr10I 1373 ACCGGC [GA]^CCGG[CT]
1703 GCCGGT
DsaI 1067 CCATGG C^C[GA][CT]GG
1353 CCATGG
EagI 96 CGGCCG C^GGCCG
1691 CGGCCG
EclXI 96 CGGCCG C^GGCCG
1691 CGGCCG
Eco52I 96 CGGCCG C^GGCCG
1691 CGGCCG
HaeII 810 GGCGCC [GA]GCGC^[CT]
902 AGCGCC
HgaI 33 GACGC GACGC(5/10)
1823 GCGTC
Hin1I 810 GGCGCC G[GA]^CG[CT]C
1822 GGCGTC
MseI 2244 TTAA T^TAA
2439 TTAA
MslI 1176 CACCTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1744 CACGGGGGTG
NcoI 1067 CCATGG C^CATGG
1353 CCATGG
NspI 479 ACATGT [GA]CATG^[CT]
2814 ACATGT
RcaI 557 TCATGA T^CATGA
2020 TCATGA
SacI 982 GAGCTC GAGCT^C
2703 GAGCTC
SmlI 119 CTCGAG C^T[CT][GA]AG
427 CTCAAG
TfiI 1544 GAATC G^A[AT]TC
1616 GATTC
Tru9I 2244 TTAA T^TAA
2439 TTAA
Tsp509I 648 AATT ^AATT
2214 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AocI AspHI AvaI AvaII BanI
BanII BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BsrBI BsrGI BsrI BssKI BssSI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspBI
StyI TaqI TseI Tsp45I TspRI
TthHB8I XhoII