################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## AatII AccIII AclI AflII AgeI Aor51HI AscI Asp700I AspI AviII BamHI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BsaAI BseAI BsiWI BspDI BspEI BssHII Bst1107I BstAPI BstZ17I ClaI CpoI DraI DraIII DrdI Eco47III EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HpaI KspI MaeII MfeI MluI MroI MunI NaeI NdeI NgoMIV NsiI PacI PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SalI ScaI SgrAI SnaBI SpeI SphI SspI SwaI Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### Acc65I 1249 GGTACC G^GTACC AccI 483 GTCTAC GT^[AC][GT]AC AcsI 2213 AAATTC [GA]^AATT[CT] AhdI 1553 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 1199 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 1759 GGGCCC GGGCC^C ApoI 2213 AAATTC [GA]^AATT[CT] AseI 2243 ATTAAT AT^TAAT AsnI 2243 ATTAAT AT^TAAT Asp718I 1249 GGTACC G^GTACC AspEI 1553 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 2116 CCTAGG C^CTAGG BalI 2752 TGGCCA TGG^CCA BbeI 810 GGCGCC GGCGC^C BlnI 2116 CCTAGG C^CTAGG BsaBI 573 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1246 TCCGGT [AT]^CCGG[AT] BsmBI 22 GAGACG CGTCTC(1/5) BsmI 500 GCATTC GAATGC(1/-1) BspMI 2568 GCAGGT ACCTGC(4/8) BsrDI 254 GCAATG GCAATG(2/0) BstBI 1780 TTCGAA TT^CGAA BstEII 723 GGTGACC G^GT[ACGT]ACC BstPI 723 GGTGACC G^GT[ACGT]ACC Eam1105I 1553 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 1674 CCTGCGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 723 GGTGACC G^GT[ACGT]ACC HindIII 4 AAGCTT A^AGCTT KasI 810 GGCGCC G^GCGCC KpnI 1249 GGTACC GGTAC^C MamI 573 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 2752 TGGCCA TGG^CCA MscI 2752 TGGCCA TGG^CCA NarI 810 GGCGCC GG^CGCC NheI 58 GCTAGC G^CTAGC NotI 1690 GCGGCCGC GC^GGCCGC NruI 1645 TCGCGA TCG^CGA NspV 1780 TTCGAA TT^CGAA PaeR7I 119 CTCGAG C^TCGAG PflMI 2599 CCAACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 2243 ATTAAT AT^TAAT PvuII 1798 CAGCTG CAG^CTG SapI 991 GAAGAGC GCTCTTC(1/4) SbfI 2565 CCTGCAGG CCTGCA^GG SexAI 41 ACCTGGT A^CC[AT]GGT SfiI 1683 GGCCACAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 810 GGCGCC GGC^GCC SfuI 1780 TTCGAA TT^CGAA SmaI 1946 CCCGGG CCC^GGG Sse8387I 2565 CCTGCAGG CCTGCA^GG StuI 695 AGGCCT AGG^CCT Van91I 2599 CCAACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1280 CCAGTCATAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 119 CTCGAG C^TCGAG XmaCI 1946 CCCGGG C^CCGGG XmaI 1946 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AcyI 810 GGCGCC G[GA]^CG[CT]C 1822 GGCGTC AflIII 479 ACATGT A^C[GA][CT]GT 2814 ACATGT Alw44I 151 GTGCAC G^TGCAC 1185 GTGCAC ApaLI 151 GTGCAC G^TGCAC 1185 GTGCAC BbsI 30 GAAGAC GAAGAC(2/6) 753 GAAGAC BpuAI 30 GAAGAC GAAGAC(2/6) 753 GAAGAC BsaHI 810 GGCGCC G[GA]^CG[CT]C 1822 GGCGTC BsaI 1370 GAGACC GGTCTC(1/5) 2403 GGTCTC BsiEI 96 CGGCCG CG[GA][CT]^CG 1691 CGGCCG BspHI 557 TCATGA T^CATGA 2020 TCATGA BspLU11I 479 ACATGT A^CATGT 2814 ACATGT BsrFI 1373 ACCGGC [GA]^CCGG[CT] 1703 GCCGGT BstXI 1061 CCAGCCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2577 CCAGGGAGCTGG Cfr10I 1373 ACCGGC [GA]^CCGG[CT] 1703 GCCGGT DsaI 1067 CCATGG C^C[GA][CT]GG 1353 CCATGG EagI 96 CGGCCG C^GGCCG 1691 CGGCCG EclXI 96 CGGCCG C^GGCCG 1691 CGGCCG Eco52I 96 CGGCCG C^GGCCG 1691 CGGCCG HaeII 810 GGCGCC [GA]GCGC^[CT] 902 AGCGCC HgaI 33 GACGC GACGC(5/10) 1823 GCGTC Hin1I 810 GGCGCC G[GA]^CG[CT]C 1822 GGCGTC MseI 2244 TTAA T^TAA 2439 TTAA MslI 1176 CACCTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1744 CACGGGGGTG NcoI 1067 CCATGG C^CATGG 1353 CCATGG NspI 479 ACATGT [GA]CATG^[CT] 2814 ACATGT RcaI 557 TCATGA T^CATGA 2020 TCATGA SacI 982 GAGCTC GAGCT^C 2703 GAGCTC SmlI 119 CTCGAG C^T[CT][GA]AG 427 CTCAAG TfiI 1544 GAATC G^A[AT]TC 1616 GATTC Tru9I 2244 TTAA T^TAA 2439 TTAA Tsp509I 648 AATT ^AATT 2214 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 97 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AocI AspHI AvaI AvaII BanI BanII BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BsrBI BsrGI BsrI BssKI BssSI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspBI StyI TaqI TseI Tsp45I TspRI TthHB8I XhoII