##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII AccI AclI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AspEI AviII BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BpuAI BsaBI BsiWI BsmBI Bsp1407I
BspDI BspHI BspLU11I BsrDI BsrGI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I ClaI CpoI DrdI Eam1105I
Eco47III EcoO65I EcoRI EcoRV EcoT22I
FbaI FspI HincII HindII HpaI
MamI MfeI MluI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RcaI
RsrII SalI ScaI SfiI SfuI
SgrAI SpeI SphI SspBI StuI
SwaI TaqI TthHB8I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 2011 GGTACC G^GTACC
AccIII 2168 TCCGGA T^CCGGA
ApaI 377 GGGCCC GGGCC^C
AseI 657 ATTAAT AT^TAAT
AsnI 657 ATTAAT AT^TAAT
Asp718I 2011 GGTACC G^GTACC
AvrII 2452 CCTAGG C^CTAGG
BalI 981 TGGCCA TGG^CCA
BlnI 2452 CCTAGG C^CTAGG
BlpI 1417 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1417 GCTCAGC GC^T[ACGT]AGC
BsaAI 2967 TACGTA [CT]AC^GT[GA]
BseAI 2168 TCCGGA T^CCGGA
BsmI 944 GAATGC GAATGC(1/-1)
BspEI 2168 TCCGGA T^CCGGA
BspMI 1709 ACCTGC ACCTGC(4/8)
CelII 1417 GCTCAGC GC^T[ACGT]AGC
DraI 916 TTTAAA TTT^AAA
DraIII 2394 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 476 CGGCCG C^GGCCG
EclXI 476 CGGCCG C^GGCCG
Eco52I 476 CGGCCG C^GGCCG
FseI 290 GGCCGGCC GGCCGG^CC
KpnI 2011 GGTACC GGTAC^C
KspI 258 CCGCGG CCGC^GG
MluNI 981 TGGCCA TGG^CCA
MroI 2168 TCCGGA T^CCGGA
MscI 981 TGGCCA TGG^CCA
NspI 752 ACATGC [GA]CATG^[CT]
PshBI 657 ATTAAT AT^TAAT
SacII 258 CCGCGG CCGC^GG
SbfI 2583 CCTGCAGG CCTGCA^GG
SexAI 614 ACCAGGT A^CC[AT]GGT
SmaI 525 CCCGGG CCC^GGG
SnaBI 2967 TACGTA TAC^GTA
Sse8387I 2583 CCTGCAGG CCTGCA^GG
SspI 2766 AATATT AAT^ATT
XcmI 1887 CCAGACACTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 525 CCCGGG C^CCGGG
XmaI 525 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcsI 2025 GAATTT [GA]^AATT[CT]
2724 AAATTT
AcyI 405 GGCGCC G[GA]^CG[CT]C
493 GGCGCC
AfaI 2012 GTAC GT^AC
2603 GTAC
ApoI 2025 GAATTT [GA]^AATT[CT]
2724 AAATTT
Asp700I 1117 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2218 GAACAAGTTC
AspI 1890 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2556 GACTTTGTC
BbeI 405 GGCGCC GGCGC^C
493 GGCGCC
BglI 53 GCCTGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1713 GCCAGGTGGGC
BglII 1119 AGATCT A^GATCT
1911 AGATCT
BsaHI 405 GGCGCC G[GA]^CG[CT]C
493 GGCGCC
BsaWI 1786 ACCGGA [AT]^CCGG[AT]
2168 TCCGGA
BsiEI 168 CGACCG CG[GA][CT]^CG
476 CGGCCG
BssHII 263 GCGCGC G^CGCGC
579 GCGCGC
BssSI 2872 CTCGTG CACGAG(-5/-1)
2935 CTCGTG
BstXI 641 CCAATACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2303 CCAGCGACCTGG
EcoNI 798 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
837 CCTACAGAAGG
Hin1I 405 GGCGCC G[GA]^CG[CT]C
493 GGCGCC
HindIII 778 AAGCTT A^AGCTT
1207 AAGCTT
KasI 405 GGCGCC G^GCGCC
493 GGCGCC
MaeII 509 ACGT A^CGT
2968 ACGT
NarI 405 GGCGCC GG^CGCC
493 GGCGCC
PflFI 1890 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2556 GACTTTGTC
PpuMI 80 AGGACCT [GA]G^G[AT]CC[CT]
816 GGGTCCT
RsaI 2012 GTAC GT^AC
2603 GTAC
SacI 685 GAGCTC GAGCT^C
2869 GAGCTC
SfoI 405 GGCGCC GGC^GCC
493 GGCGCC
TfiI 2342 GAATC G^A[AT]TC
2859 GATTC
Tth111I 1890 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2556 GACTTTGTC
XmnI 1117 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2218 GAACAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
AocI AspHI AvaI AvaII BamHI
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
PflMI PleI PstI PvuII SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XhoII