##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflII
AgeI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspI
AviII BbeI BbrPI BbvCI BcgI
BciVI BfrI BglI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BspDI
BspEI BsrBI BsrFI BssHII BstEII
BstPI CelII Cfr10I ClaI CpoI
DraIII EagI EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRV EcoT22I FauI
FseI FspI Hin1I HindIII HpaI
KasI KpnI KspI MamI MfeI
MroI MunI NaeI NarI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmaCI PmlI
PshBI PvuI RsrII SacI SacII
SbfI SexAI SfiI SfoI SgrAI
SnaBI SpeI Sse8387I StuI SwaI
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccII 2734 CGCG CG^CG
AhdI 1070 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1694 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 293 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1070 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2157 CCCGGG C^[CT]CG[GA]G
AvrII 2958 CCTAGG C^CTAGG
BalI 799 TGGCCA TGG^CCA
BanI 2537 GGCACC G^G[CT][GA]CC
BlnI 2958 CCTAGG C^CTAGG
BseRI 381 GAGGAG GAGGAG(10/8)
BsoBI 2157 CCCGGG C^[CT]CG[GA]G
Bsp1407I 3132 TGTACA T^GTACA
BsrGI 3132 TGTACA T^GTACA
BssSI 39 CACGAG CACGAG(-5/-1)
Bst1107I 1426 GTATAC GTA^TAC
BstBI 300 TTCGAA TT^CGAA
BstUI 2734 CGCG CG^CG
BstXI 3392 CCACAAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1426 GTATAC GTA^TAC
DrdI 2732 GACGCGTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 799 TGGCCA [CT]^GGCC[GA]
Eam1105I 1070 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2301 GAATTC G^AATTC
HaeII 188 AGCGCC [GA]GCGC^[CT]
MluI 2733 ACGCGT A^CGCGT
MluNI 799 TGGCCA TGG^CCA
MscI 799 TGGCCA TGG^CCA
MspA1I 1310 CAGCTG C[AC]G^C[GT]G
MvnI 2734 CGCG CG^CG
NdeI 2681 CATATG CA^TATG
NspV 300 TTCGAA TT^CGAA
PflMI 1185 CCATTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmeI 838 GTTTAAAC GTTT^AAAC
PshAI 1087 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1310 CAGCTG CAG^CTG
SalI 1379 GTCGAC G^TCGAC
SapI 597 GCTCTTC GCTCTTC(1/4)
ScaI 3313 AGTACT AGT^ACT
SfuI 300 TTCGAA TT^CGAA
SmaI 2157 CCCGGG CCC^GGG
SphI 2933 GCATGC GCATG^C
SspBI 3132 TGTACA T^GTACA
Van91I 1185 CCATTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1532 TCTAGA T^CTAGA
XmaCI 2157 CCCGGG C^CCGGG
XmaI 2157 CCCGGG C^CCGGG
XmnI 293 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AciI 903 CCGC CCGC(-3/-1)
1975 CCGC
AspHI 363 GTGCTC G[AT]GC[AT]^C
3352 GTGCTC
BamHI 61 GGATCC G^GATCC
2196 GGATCC
BbsI 2020 GAAGAC GAAGAC(2/6)
3189 GAAGAC
BclI 261 TGATCA T^GATCA
1140 TGATCA
BpuAI 2020 GAAGAC GAAGAC(2/6)
3189 GAAGAC
BsiHKAI 363 GTGCTC G[AT]GC[AT]^C
3352 GTGCTC
BspHI 1737 TCATGA T^CATGA
2169 TCATGA
BspLU11I 859 ACATGT A^CATGT
2754 ACATGT
BspMI 1383 ACCTGC ACCTGC(4/8)
3418 GCAGGT
BsrDI 2409 GCAATG GCAATG(2/0)
3128 GCAATG
BstAPI 1849 GCAGGACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1999 GCATGGCTTGC
FbaI 261 TGATCA T^GATCA
1140 TGATCA
HgaI 2732 GACGC GACGC(5/10)
2735 GCGTC
HincII 986 GTTGAC GT[CT]^[GA]AC
1379 GTCGAC
HindII 986 GTTGAC GT[CT]^[GA]AC
1379 GTCGAC
RcaI 1737 TCATGA T^CATGA
2169 TCATGA
XcmI 1185 CCATTTCATGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2034 CCATGAAAATCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccI AclI AcsI AfaI AflIII
AluI AlwI AocI ApoI AvaII
BanII BbvI BcnI BfaI BglII
BmyI BpmI BsaI BsaJI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EarI
Eco81I EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PpuMI
Psp1406I PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII