################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## AatII Acc65I AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BcgI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsiWI BspHI BspLU11I BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstPI BstXI Bsu36I CelII Cfr10I CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV FseI FspI HaeII HincII HindII HpaI KasI KpnI KspI MamI MfeI MluI MunI NaeI NarI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI Psp1406I PstI RcaI RsrII SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I StuI SwaI Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AccIII 633 TCCGGA T^CCGGA AcyI 447 GACGCC G[GA]^CG[CT]C ApaI 1220 GGGCCC GGGCC^C AvaI 1418 CCCGGG C^[CT]CG[GA]G BalI 1663 TGGCCA TGG^CCA BbvCI 393 CCTCAGC CCTCAGC(-5/-2) BciVI 485 GGATAC GTATCC(6/5) BclI 2244 TGATCA T^GATCA BglI 1417 GCCCGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 2180 AGATCT A^GATCT BsaHI 447 GACGCC G[GA]^CG[CT]C BsaWI 633 TCCGGA [AT]^CCGG[AT] BseAI 633 TCCGGA T^CCGGA BseRI 102 GAGGAG GAGGAG(10/8) BsiEI 192 CGATCG CG[GA][CT]^CG BsmBI 33 CGTCTC CGTCTC(1/5) BsmFI 229 GGGAC GGGAC(10/14) BsmI 1192 GAATGC GAATGC(1/-1) BsoBI 1418 CCCGGG C^[CT]CG[GA]G Bsp1407I 497 TGTACA T^GTACA BspDI 190 ATCGAT AT^CGAT BspEI 633 TCCGGA T^CCGGA BsrDI 1596 GCAATG GCAATG(2/0) BsrGI 497 TGTACA T^GTACA BstYI 2180 AGATCT [GA]^GATC[CT] ClaI 190 ATCGAT AT^CGAT DsaI 166 CCATGG C^C[GA][CT]GG EaeI 1663 TGGCCA [CT]^GGCC[GA] EcoT22I 1985 ATGCAT ATGCA^T FbaI 2244 TGATCA T^GATCA Hin1I 447 GACGCC G[GA]^CG[CT]C HindIII 2073 AAGCTT A^AGCTT MaeII 1126 ACGT A^CGT MflI 2180 AGATCT [GA]^GATC[CT] MluNI 1663 TGGCCA TGG^CCA MroI 633 TCCGGA T^CCGGA MscI 1663 TGGCCA TGG^CCA MspA1I 1412 CAGCTG C[AC]G^C[GT]G NcoI 166 CCATGG C^CATGG NdeI 1199 CATATG CA^TATG NheI 1432 GCTAGC G^CTAGC NsiI 1985 ATGCAT ATGCA^T NspI 658 ACATGC [GA]CATG^[CT] PflMI 2114 CCATTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 243 AGGACCC [GA]G^G[AT]CC[CT] PshAI 2102 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 192 CGATCG CGAT^CG PvuII 1412 CAGCTG CAG^CTG SapI 143 GCTCTTC GCTCTTC(1/4) SfaNI 920 GCATC GCATC(5/9) SmaI 1418 CCCGGG CCC^GGG SspBI 497 TGTACA T^GTACA SspI 813 AATATT AAT^ATT TfiI 1329 GATTC G^A[AT]TC Van91I 2114 CCATTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 2180 AGATCT [GA]^GATC[CT] XmaCI 1418 CCCGGG C^CCGGG XmaI 1418 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccI 574 GTATAC GT^[AC][GT]AC 690 GTATAC AlwI 309 GGATC GGATC(4/5) 781 GGATC AlwNI 301 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG 1146 CAGTCACTG AseI 810 ATTAAT AT^TAAT 2303 ATTAAT AsnI 810 ATTAAT AT^TAAT 2303 ATTAAT AvaII 230 GGACC G^G[AT]CC 244 GGACC BanI 130 GGCACC G^G[CT][GA]CC 642 GGTGCC BbsI 679 GAAGAC GAAGAC(2/6) 2232 GTCTTC BpmI 1409 CTCCAG CTGGAG(16/14) 953 CTGGAG BpuAI 679 GAAGAC GAAGAC(2/6) 2232 GTCTTC Bst1107I 574 GTATAC GTA^TAC 690 GTATAC BstF5I 1678 CATCC GGATG(2/0) 443 GGATG BstZ17I 574 GTATAC GTA^TAC 690 GTATAC Cac8I 1001 GCCAGC GC[ACGT]^[ACGT]GC 1432 GCTAGC EcoRI 1312 GAATTC G^AATTC 1657 GAATTC FauI 156 CCCGC CCCGC(4/6) 98 GCGGG FokI 1678 CATCC GGATG(9/13) 443 GGATG HgaI 447 GACGC GACGC(5/10) 740 GCGTC MslI 200 CACCGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1746 CATATTGGTG PleI 1261 GAGTC GAGTC(4/5) 2222 GAGTC PshBI 810 ATTAAT AT^TAAT 2303 ATTAAT SacI 141 GAGCTC GAGCT^C 956 GAGCTC ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AccII AciI AcsI AfaI AluI ApoI AspHI BanII BbvI BcnI BfaI BmyI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI Bsp1286I BspMI BsrI BssKI BstNI BstUI CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I