##################################
# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbrPI BcgI BfrI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsiWI
BspHI BspLU11I BsrBI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstPI
BstXI Bsu36I CelII Cfr10I CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV FseI FspI HaeII
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MluI MunI
NaeI NarI NgoMIV NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI Psp1406I PstI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I StuI SwaI Tth111I
XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccIII 633 TCCGGA T^CCGGA
AcyI 447 GACGCC G[GA]^CG[CT]C
ApaI 1220 GGGCCC GGGCC^C
AvaI 1418 CCCGGG C^[CT]CG[GA]G
BalI 1663 TGGCCA TGG^CCA
BbvCI 393 CCTCAGC CCTCAGC(-5/-2)
BciVI 485 GGATAC GTATCC(6/5)
BclI 2244 TGATCA T^GATCA
BglI 1417 GCCCGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2180 AGATCT A^GATCT
BsaHI 447 GACGCC G[GA]^CG[CT]C
BsaWI 633 TCCGGA [AT]^CCGG[AT]
BseAI 633 TCCGGA T^CCGGA
BseRI 102 GAGGAG GAGGAG(10/8)
BsiEI 192 CGATCG CG[GA][CT]^CG
BsmBI 33 CGTCTC CGTCTC(1/5)
BsmFI 229 GGGAC GGGAC(10/14)
BsmI 1192 GAATGC GAATGC(1/-1)
BsoBI 1418 CCCGGG C^[CT]CG[GA]G
Bsp1407I 497 TGTACA T^GTACA
BspDI 190 ATCGAT AT^CGAT
BspEI 633 TCCGGA T^CCGGA
BsrDI 1596 GCAATG GCAATG(2/0)
BsrGI 497 TGTACA T^GTACA
BstYI 2180 AGATCT [GA]^GATC[CT]
ClaI 190 ATCGAT AT^CGAT
DsaI 166 CCATGG C^C[GA][CT]GG
EaeI 1663 TGGCCA [CT]^GGCC[GA]
EcoT22I 1985 ATGCAT ATGCA^T
FbaI 2244 TGATCA T^GATCA
Hin1I 447 GACGCC G[GA]^CG[CT]C
HindIII 2073 AAGCTT A^AGCTT
MaeII 1126 ACGT A^CGT
MflI 2180 AGATCT [GA]^GATC[CT]
MluNI 1663 TGGCCA TGG^CCA
MroI 633 TCCGGA T^CCGGA
MscI 1663 TGGCCA TGG^CCA
MspA1I 1412 CAGCTG C[AC]G^C[GT]G
NcoI 166 CCATGG C^CATGG
NdeI 1199 CATATG CA^TATG
NheI 1432 GCTAGC G^CTAGC
NsiI 1985 ATGCAT ATGCA^T
NspI 658 ACATGC [GA]CATG^[CT]
PflMI 2114 CCATTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 243 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 2102 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 192 CGATCG CGAT^CG
PvuII 1412 CAGCTG CAG^CTG
SapI 143 GCTCTTC GCTCTTC(1/4)
SfaNI 920 GCATC GCATC(5/9)
SmaI 1418 CCCGGG CCC^GGG
SspBI 497 TGTACA T^GTACA
SspI 813 AATATT AAT^ATT
TfiI 1329 GATTC G^A[AT]TC
Van91I 2114 CCATTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 2180 AGATCT [GA]^GATC[CT]
XmaCI 1418 CCCGGG C^CCGGG
XmaI 1418 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 574 GTATAC GT^[AC][GT]AC
690 GTATAC
AlwI 309 GGATC GGATC(4/5)
781 GGATC
AlwNI 301 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1146 CAGTCACTG
AseI 810 ATTAAT AT^TAAT
2303 ATTAAT
AsnI 810 ATTAAT AT^TAAT
2303 ATTAAT
AvaII 230 GGACC G^G[AT]CC
244 GGACC
BanI 130 GGCACC G^G[CT][GA]CC
642 GGTGCC
BbsI 679 GAAGAC GAAGAC(2/6)
2232 GTCTTC
BpmI 1409 CTCCAG CTGGAG(16/14)
953 CTGGAG
BpuAI 679 GAAGAC GAAGAC(2/6)
2232 GTCTTC
Bst1107I 574 GTATAC GTA^TAC
690 GTATAC
BstF5I 1678 CATCC GGATG(2/0)
443 GGATG
BstZ17I 574 GTATAC GTA^TAC
690 GTATAC
Cac8I 1001 GCCAGC GC[ACGT]^[ACGT]GC
1432 GCTAGC
EcoRI 1312 GAATTC G^AATTC
1657 GAATTC
FauI 156 CCCGC CCCGC(4/6)
98 GCGGG
FokI 1678 CATCC GGATG(9/13)
443 GGATG
HgaI 447 GACGC GACGC(5/10)
740 GCGTC
MslI 200 CACCGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1746 CATATTGGTG
PleI 1261 GAGTC GAGTC(4/5)
2222 GAGTC
PshBI 810 ATTAAT AT^TAAT
2303 ATTAAT
SacI 141 GAGCTC GAGCT^C
956 GAGCTC
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI AspHI BanII BbvI BcnI
BfaI BmyI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BspMI
BsrI BssKI BstNI BstUI CfoI
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII EcoT14I Fnu4HI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I