##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AocI ApaI ApoI AscI AseI
AsnI Asp718I AspI AvrII BbrPI
BfrI BlnI BsaAI BsaBI BsiEI
BsiWI BsmBI BsmI BspDI BsrDI
BssHII BssSI Bst1107I BstBI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
EagI EclXI Eco52I Eco81I EcoRI
EcoRV EcoT22I FseI HgaI HpaI
KpnI KspI MamI MfeI MluI
MunI NdeI NotI NruI NsiI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AatII 1707 GACGTC GACGT^C
AccIII 1444 TCCGGA T^CCGGA
AgeI 433 ACCGGT A^CCGGT
AhdI 324 GACCCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 99 GTGCAC G^TGCAC
Aor51HI 584 AGCGCT AGC^GCT
ApaLI 99 GTGCAC G^TGCAC
Asp700I 1613 GAAGCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 324 GACCCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1870 TGCGCA TGC^GCA
BamHI 1441 GGATCC G^GATCC
BciVI 2846 GTATCC GTATCC(6/5)
BseAI 1444 TCCGGA T^CCGGA
BspEI 1444 TCCGGA T^CCGGA
BspHI 1105 TCATGA T^CATGA
BsrBI 2687 GAGCGG CCGCTC(-3/-3)
BstAPI 284 GCAGACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 1579 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 324 GACCCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 584 AGCGCT AGC^GCT
FspI 1870 TGCGCA TGC^GCA
HindIII 105 AAGCTT A^AGCTT
MroI 1444 TCCGGA T^CCGGA
NheI 665 GCTAGC G^CTAGC
PinAI 433 ACCGGT A^CCGGT
RcaI 1105 TCATGA T^CATGA
XcmI 2436 CCAGTTTAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1613 GAAGCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccII 985 CGCG CG^CG
1526 CGCG
BbvCI 146 CCTCAGC CCTCAGC(-5/-2)
2364 CCTCAGC
BclI 467 TGATCA T^GATCA
805 TGATCA
BglII 510 AGATCT A^GATCT
1019 AGATCT
BsaWI 433 ACCGGT [AT]^CCGG[AT]
1444 TCCGGA
BspLU11I 192 ACATGT A^CATGT
263 ACATGT
BstUI 985 CGCG CG^CG
1526 CGCG
FauI 378 GCGGG CCCGC(4/6)
1757 GCGGG
FbaI 467 TGATCA T^GATCA
805 TGATCA
HincII 726 GTCAAC GT[CT]^[GA]AC
1113 GTCAAC
HindII 726 GTCAAC GT[CT]^[GA]AC
1113 GTCAAC
MaeII 1708 ACGT A^CGT
3177 ACGT
MvnI 985 CGCG CG^CG
1526 CGCG
NcoI 616 CCATGG C^CATGG
2105 CCATGG
SapI 2340 GCTCTTC GCTCTTC(1/4)
3125 GCTCTTC
SmaI 327 CCCGGG CCC^GGG
1686 CCCGGG
StuI 1291 AGGCCT AGG^CCT
3067 AGGCCT
TfiI 752 GAATC G^A[AT]TC
1618 GATTC
XmaCI 327 CCCGGG C^CCGGG
1686 CCCGGG
XmaI 327 CCCGGG C^CCGGG
1686 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AciI AcyI AfaI AflIII AluI
AlwI AlwNI AspHI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvI BcgI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BspMI BsrFI
BsrGI BsrI BssKI BstEII BstF5I
BstNI BstPI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
SspBI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII