################################## # NO CUT ENZYMES ( 84 enzymes) # ################################## Acc65I AccI AclI AcsI AflII AocI ApaI ApoI AscI AseI AsnI Asp718I AspI AvrII BbrPI BfrI BlnI BsaAI BsaBI BsiEI BsiWI BsmBI BsmI BspDI BsrDI BssHII BssSI Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DraI DrdI EagI EclXI Eco52I Eco81I EcoRI EcoRV EcoT22I FseI HgaI HpaI KpnI KspI MamI MfeI MluI MunI NdeI NotI NruI NsiI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 28 enzymes) # ################################### AatII 1707 GACGTC GACGT^C AccIII 1444 TCCGGA T^CCGGA AgeI 433 ACCGGT A^CCGGT AhdI 324 GACCCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 99 GTGCAC G^TGCAC Aor51HI 584 AGCGCT AGC^GCT ApaLI 99 GTGCAC G^TGCAC Asp700I 1613 GAAGCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 324 GACCCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 1870 TGCGCA TGC^GCA BamHI 1441 GGATCC G^GATCC BciVI 2846 GTATCC GTATCC(6/5) BseAI 1444 TCCGGA T^CCGGA BspEI 1444 TCCGGA T^CCGGA BspHI 1105 TCATGA T^CATGA BsrBI 2687 GAGCGG CCGCTC(-3/-3) BstAPI 284 GCAGACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DraIII 1579 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 324 GACCCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 584 AGCGCT AGC^GCT FspI 1870 TGCGCA TGC^GCA HindIII 105 AAGCTT A^AGCTT MroI 1444 TCCGGA T^CCGGA NheI 665 GCTAGC G^CTAGC PinAI 433 ACCGGT A^CCGGT RcaI 1105 TCATGA T^CATGA XcmI 2436 CCAGTTTAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 1613 GAAGCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccII 985 CGCG CG^CG 1526 CGCG BbvCI 146 CCTCAGC CCTCAGC(-5/-2) 2364 CCTCAGC BclI 467 TGATCA T^GATCA 805 TGATCA BglII 510 AGATCT A^GATCT 1019 AGATCT BsaWI 433 ACCGGT [AT]^CCGG[AT] 1444 TCCGGA BspLU11I 192 ACATGT A^CATGT 263 ACATGT BstUI 985 CGCG CG^CG 1526 CGCG FauI 378 GCGGG CCCGC(4/6) 1757 GCGGG FbaI 467 TGATCA T^GATCA 805 TGATCA HincII 726 GTCAAC GT[CT]^[GA]AC 1113 GTCAAC HindII 726 GTCAAC GT[CT]^[GA]AC 1113 GTCAAC MaeII 1708 ACGT A^CGT 3177 ACGT MvnI 985 CGCG CG^CG 1526 CGCG NcoI 616 CCATGG C^CATGG 2105 CCATGG SapI 2340 GCTCTTC GCTCTTC(1/4) 3125 GCTCTTC SmaI 327 CCCGGG CCC^GGG 1686 CCCGGG StuI 1291 AGGCCT AGG^CCT 3067 AGGCCT TfiI 752 GAATC G^A[AT]TC 1618 GATTC XmaCI 327 CCCGGG C^CCGGG 1686 CCCGGG XmaI 327 CCCGGG C^CCGGG 1686 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 126 enzymes) # ######################################### AciI AcyI AfaI AflIII AluI AlwI AlwNI AspHI AvaI AvaII BalI BanI BanII BbeI BbsI BbvI BcgI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrFI BsrGI BsrI BssKI BstEII BstF5I BstNI BstPI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoO65I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI SspBI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII