##################################
# NO CUT ENZYMES (   87 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AgeI        AhdI        Alw44I      Aor51HI     ApaLI       
ApoI        AscI        Asp718I     AspEI       AviII       
AvrII       BbeI        BcgI        BglII       BlnI        
BseAI       BsiWI       BsmBI       BsmI        BspDI       
BspEI       BspHI       BspLU11I    BsrDI       BssSI       
Bst1107I    BstBI       BstZ17I     ClaI        CpoI        
DraI        Eam1105I    Eco47III    EcoRI       EcoRV       
EcoT22I     FseI        FspI        HindIII     HpaI        
KasI        KpnI        KspI        MfeI        MluI        
MroI        MunI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PinAI       
PmeI        PpuMI       PshAI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SexAI       
SfoI        SfuI        SgrAI       SnaBI       SpeI        
SphI        SspI        StuI        SwaI        Tsp509I     
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AcyI          449  GACGCC               G[GA]^CG[CT]C
AflII        1967  CTTAAG               C^TTAAG
AflIII        806  ACGTGT               A^C[GA][CT]GT
ApaI         2982  GGGCCC               GGGCC^C
AseI         3202  ATTAAT               AT^TAAT
AsnI         3202  ATTAAT               AT^TAAT
Asp700I      1138  GAAAGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BfrI         1967  CTTAAG               C^TTAAG
BsaBI         794  GATGCGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         449  GACGCC               G[GA]^CG[CT]C
BsaI         1607  GGTCTC               GGTCTC(1/5)
BsgI          629  CTGCAC               GTGCAG(16/14)
BsiEI        1815  CGGCCG               CG[GA][CT]^CG
Bsp1407I     2184  TGTACA               T^GTACA
BsrBI         992  GAGCGG               CCGCTC(-3/-3)
BsrFI        1983  ACCGGC               [GA]^CCGG[CT]
BsrGI        2184  TGTACA               T^GTACA
BstAPI       1920  GCATCACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I       1983  ACCGGC               [GA]^CCGG[CT]
DraIII       1973  CACATGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         2470  GACAAGCTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         1815  CGGCCG               C^GGCCG
EclXI        1815  CGGCCG               C^GGCCG
Eco52I       1815  CGGCCG               C^GGCCG
HaeII         105  AGCGCC               [GA]GCGC^[CT]
Hin1I         449  GACGCC               G[GA]^CG[CT]C
HincII        536  GTCAAC               GT[CT]^[GA]AC
HindII        536  GTCAAC               GT[CT]^[GA]AC
MamI          794  GATGCGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PshBI        3202  ATTAAT               AT^TAAT
PvuII         827  CAGCTG               CAG^CTG
SacI         1211  GAGCTC               GAGCT^C
SbfI          910  CCTGCAGG             CCTGCA^GG
ScaI         2991  AGTACT               AGT^ACT
SfiI         1807  GGCCAAGACGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI            5  CCCGGG               CCC^GGG
Sse8387I      910  CCTGCAGG             CCTGCA^GG
SspBI        2184  TGTACA               T^GTACA
XmaCI           5  CCCGGG               C^CCGGG
XmaI            5  CCCGGG               C^CCGGG
XmnI         1138  GAAAGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AocI          348  CCTCAGG              CC^T[ACGT]AGG
             1253  CCTGAGG             
AspHI         170  GTGCTC               G[AT]GC[AT]^C
             1211  GAGCTC              
AspI         1525  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2892  GACTTAGTC           
AvaI            5  CCCGGG               C^[CT]CG[GA]G
              544  CTCGGG              
BanI         1703  GGTGCC               G^G[CT][GA]CC
             2372  GGTGCC              
BbrPI         805  CACGTG               CAC^GTG
             2276  CACGTG              
BciVI         799  GTATCC               GTATCC(6/5)
             2628  GTATCC              
BclI          501  TGATCA               T^GATCA
             1800  TGATCA              
BglI         1767  GCCTGCCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1808  GCCAAGACGGC         
BlpI          845  GCTAAGC              GC^T[ACGT]AGC
             2921  GCTTAGC             
Bpu1102I      845  GCTAAGC              GC^T[ACGT]AGC
             2921  GCTTAGC             
BsaAI         805  CACGTG               [CT]AC^GT[GA]
             2276  CACGTG              
BsiHKAI       170  GTGCTC               G[AT]GC[AT]^C
             1211  GAGCTC              
BsoBI           5  CCCGGG               C^[CT]CG[GA]G
              544  CTCGGG              
BstXI        1503  CCACGCTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3215  CCACTGGGCTGG        
Bsu36I        348  CCTCAGG              CC^T[ACGT]AGG
             1253  CCTGAGG             
CelII         845  GCTAAGC              GC^T[ACGT]AGC
             2921  GCTTAGC             
Eco81I        348  CCTCAGG              CC^T[ACGT]AGG
             1253  CCTGAGG             
EcoNI        1665  CCTCAATCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2328  CCTCAGAGAGG         
FbaI          501  TGATCA               T^GATCA
             1800  TGATCA              
PflFI        1525  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2892  GACTTAGTC           
PmaCI         805  CACGTG               CAC^GTG
             2276  CACGTG              
PmlI          805  CACGTG               CAC^GTG
             2276  CACGTG              
TaqI         1021  TCGA                 T^CGA
             1556  TCGA                
Tth111I      1525  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2892  GACTTAGTC           
TthHB8I      1021  TCGA                 T^CGA
             1556  TCGA                
XcmI          804  CCACGTGTGAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             3215  CCACTGGGCTGGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  103 enzymes) #
#########################################

AccI        AccII       AciI        AfaI        AluI        
AlwI        AlwNI       AvaII       BalI        BamHI       
BanII       BbsI        BbvCI       BbvI        BcnI        
BfaI        BmyI        BpmI        BpuAI       BsaJI       
BsaWI       BseRI       BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BspMI       BsrI        BssHII      
BssKI       BstEII      BstF5I      BstNI       BstPI       
BstUI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoO109I    EcoO65I     EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HgaI        HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       PflMI       
PleI        PstI        RsaI        SapI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TfiI        Tru9I       TseI        Tsp45I      
TspRI       Van91I      XhoII