################################## # NO CUT ENZYMES ( 87 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI Asp718I AspEI AviII AvrII BbeI BcgI BglII BlnI BseAI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrDI BssSI Bst1107I BstBI BstZ17I ClaI CpoI DraI Eam1105I Eco47III EcoRI EcoRV EcoT22I FseI FspI HindIII HpaI KasI KpnI KspI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmeI PpuMI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SexAI SfoI SfuI SgrAI SnaBI SpeI SphI SspI StuI SwaI Tsp509I XbaI XhoI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AcyI 449 GACGCC G[GA]^CG[CT]C AflII 1967 CTTAAG C^TTAAG AflIII 806 ACGTGT A^C[GA][CT]GT ApaI 2982 GGGCCC GGGCC^C AseI 3202 ATTAAT AT^TAAT AsnI 3202 ATTAAT AT^TAAT Asp700I 1138 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BfrI 1967 CTTAAG C^TTAAG BsaBI 794 GATGCGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 449 GACGCC G[GA]^CG[CT]C BsaI 1607 GGTCTC GGTCTC(1/5) BsgI 629 CTGCAC GTGCAG(16/14) BsiEI 1815 CGGCCG CG[GA][CT]^CG Bsp1407I 2184 TGTACA T^GTACA BsrBI 992 GAGCGG CCGCTC(-3/-3) BsrFI 1983 ACCGGC [GA]^CCGG[CT] BsrGI 2184 TGTACA T^GTACA BstAPI 1920 GCATCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cfr10I 1983 ACCGGC [GA]^CCGG[CT] DraIII 1973 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 2470 GACAAGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1815 CGGCCG C^GGCCG EclXI 1815 CGGCCG C^GGCCG Eco52I 1815 CGGCCG C^GGCCG HaeII 105 AGCGCC [GA]GCGC^[CT] Hin1I 449 GACGCC G[GA]^CG[CT]C HincII 536 GTCAAC GT[CT]^[GA]AC HindII 536 GTCAAC GT[CT]^[GA]AC MamI 794 GATGCGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC PshBI 3202 ATTAAT AT^TAAT PvuII 827 CAGCTG CAG^CTG SacI 1211 GAGCTC GAGCT^C SbfI 910 CCTGCAGG CCTGCA^GG ScaI 2991 AGTACT AGT^ACT SfiI 1807 GGCCAAGACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 5 CCCGGG CCC^GGG Sse8387I 910 CCTGCAGG CCTGCA^GG SspBI 2184 TGTACA T^GTACA XmaCI 5 CCCGGG C^CCGGG XmaI 5 CCCGGG C^CCGGG XmnI 1138 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AocI 348 CCTCAGG CC^T[ACGT]AGG 1253 CCTGAGG AspHI 170 GTGCTC G[AT]GC[AT]^C 1211 GAGCTC AspI 1525 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2892 GACTTAGTC AvaI 5 CCCGGG C^[CT]CG[GA]G 544 CTCGGG BanI 1703 GGTGCC G^G[CT][GA]CC 2372 GGTGCC BbrPI 805 CACGTG CAC^GTG 2276 CACGTG BciVI 799 GTATCC GTATCC(6/5) 2628 GTATCC BclI 501 TGATCA T^GATCA 1800 TGATCA BglI 1767 GCCTGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1808 GCCAAGACGGC BlpI 845 GCTAAGC GC^T[ACGT]AGC 2921 GCTTAGC Bpu1102I 845 GCTAAGC GC^T[ACGT]AGC 2921 GCTTAGC BsaAI 805 CACGTG [CT]AC^GT[GA] 2276 CACGTG BsiHKAI 170 GTGCTC G[AT]GC[AT]^C 1211 GAGCTC BsoBI 5 CCCGGG C^[CT]CG[GA]G 544 CTCGGG BstXI 1503 CCACGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3215 CCACTGGGCTGG Bsu36I 348 CCTCAGG CC^T[ACGT]AGG 1253 CCTGAGG CelII 845 GCTAAGC GC^T[ACGT]AGC 2921 GCTTAGC Eco81I 348 CCTCAGG CC^T[ACGT]AGG 1253 CCTGAGG EcoNI 1665 CCTCAATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2328 CCTCAGAGAGG FbaI 501 TGATCA T^GATCA 1800 TGATCA PflFI 1525 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2892 GACTTAGTC PmaCI 805 CACGTG CAC^GTG 2276 CACGTG PmlI 805 CACGTG CAC^GTG 2276 CACGTG TaqI 1021 TCGA T^CGA 1556 TCGA Tth111I 1525 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2892 GACTTAGTC TthHB8I 1021 TCGA T^CGA 1556 TCGA XcmI 804 CCACGTGTGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3215 CCACTGGGCTGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 103 enzymes) # ######################################### AccI AccII AciI AfaI AluI AlwI AlwNI AvaII BalI BamHI BanII BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BsaWI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssHII BssKI BstEII BstF5I BstNI BstPI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PflMI PleI PstI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I TspRI Van91I XhoII