##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AgeI AhdI Alw44I Aor51HI ApaLI
ApoI AscI Asp718I AspEI AviII
AvrII BbeI BcgI BglII BlnI
BseAI BsiWI BsmBI BsmI BspDI
BspEI BspHI BspLU11I BsrDI BssSI
Bst1107I BstBI BstZ17I ClaI CpoI
DraI Eam1105I Eco47III EcoRI EcoRV
EcoT22I FseI FspI HindIII HpaI
KasI KpnI KspI MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PinAI
PmeI PpuMI PshAI Psp1406I PvuI
RcaI RsrII SacII SalI SexAI
SfoI SfuI SgrAI SnaBI SpeI
SphI SspI StuI SwaI Tsp509I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AcyI 449 GACGCC G[GA]^CG[CT]C
AflII 1967 CTTAAG C^TTAAG
AflIII 806 ACGTGT A^C[GA][CT]GT
ApaI 2982 GGGCCC GGGCC^C
AseI 3202 ATTAAT AT^TAAT
AsnI 3202 ATTAAT AT^TAAT
Asp700I 1138 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BfrI 1967 CTTAAG C^TTAAG
BsaBI 794 GATGCGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 449 GACGCC G[GA]^CG[CT]C
BsaI 1607 GGTCTC GGTCTC(1/5)
BsgI 629 CTGCAC GTGCAG(16/14)
BsiEI 1815 CGGCCG CG[GA][CT]^CG
Bsp1407I 2184 TGTACA T^GTACA
BsrBI 992 GAGCGG CCGCTC(-3/-3)
BsrFI 1983 ACCGGC [GA]^CCGG[CT]
BsrGI 2184 TGTACA T^GTACA
BstAPI 1920 GCATCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 1983 ACCGGC [GA]^CCGG[CT]
DraIII 1973 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2470 GACAAGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1815 CGGCCG C^GGCCG
EclXI 1815 CGGCCG C^GGCCG
Eco52I 1815 CGGCCG C^GGCCG
HaeII 105 AGCGCC [GA]GCGC^[CT]
Hin1I 449 GACGCC G[GA]^CG[CT]C
HincII 536 GTCAAC GT[CT]^[GA]AC
HindII 536 GTCAAC GT[CT]^[GA]AC
MamI 794 GATGCGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PshBI 3202 ATTAAT AT^TAAT
PvuII 827 CAGCTG CAG^CTG
SacI 1211 GAGCTC GAGCT^C
SbfI 910 CCTGCAGG CCTGCA^GG
ScaI 2991 AGTACT AGT^ACT
SfiI 1807 GGCCAAGACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 5 CCCGGG CCC^GGG
Sse8387I 910 CCTGCAGG CCTGCA^GG
SspBI 2184 TGTACA T^GTACA
XmaCI 5 CCCGGG C^CCGGG
XmaI 5 CCCGGG C^CCGGG
XmnI 1138 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AocI 348 CCTCAGG CC^T[ACGT]AGG
1253 CCTGAGG
AspHI 170 GTGCTC G[AT]GC[AT]^C
1211 GAGCTC
AspI 1525 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2892 GACTTAGTC
AvaI 5 CCCGGG C^[CT]CG[GA]G
544 CTCGGG
BanI 1703 GGTGCC G^G[CT][GA]CC
2372 GGTGCC
BbrPI 805 CACGTG CAC^GTG
2276 CACGTG
BciVI 799 GTATCC GTATCC(6/5)
2628 GTATCC
BclI 501 TGATCA T^GATCA
1800 TGATCA
BglI 1767 GCCTGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1808 GCCAAGACGGC
BlpI 845 GCTAAGC GC^T[ACGT]AGC
2921 GCTTAGC
Bpu1102I 845 GCTAAGC GC^T[ACGT]AGC
2921 GCTTAGC
BsaAI 805 CACGTG [CT]AC^GT[GA]
2276 CACGTG
BsiHKAI 170 GTGCTC G[AT]GC[AT]^C
1211 GAGCTC
BsoBI 5 CCCGGG C^[CT]CG[GA]G
544 CTCGGG
BstXI 1503 CCACGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3215 CCACTGGGCTGG
Bsu36I 348 CCTCAGG CC^T[ACGT]AGG
1253 CCTGAGG
CelII 845 GCTAAGC GC^T[ACGT]AGC
2921 GCTTAGC
Eco81I 348 CCTCAGG CC^T[ACGT]AGG
1253 CCTGAGG
EcoNI 1665 CCTCAATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2328 CCTCAGAGAGG
FbaI 501 TGATCA T^GATCA
1800 TGATCA
PflFI 1525 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2892 GACTTAGTC
PmaCI 805 CACGTG CAC^GTG
2276 CACGTG
PmlI 805 CACGTG CAC^GTG
2276 CACGTG
TaqI 1021 TCGA T^CGA
1556 TCGA
Tth111I 1525 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2892 GACTTAGTC
TthHB8I 1021 TCGA T^CGA
1556 TCGA
XcmI 804 CCACGTGTGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3215 CCACTGGGCTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccI AccII AciI AfaI AluI
AlwI AlwNI AvaII BalI BamHI
BanII BbsI BbvCI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BsaWI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssHII
BssKI BstEII BstF5I BstNI BstPI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PflMI
PleI PstI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TfiI Tru9I TseI Tsp45I
TspRI Van91I XhoII