################################## # NO CUT ENZYMES ( 63 enzymes) # ################################## Acc65I AclI AgeI Aor51HI ApaI AscI AseI AsnI Asp718I AviII AvrII BamHI BbvCI BfaI BglII BlnI BsaBI BsaI BsiWI BspDI BsrDI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI Eco47III EcoO65I FseI FspI KpnI MaeI MamI MluI NdeI NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RsrII SalI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccII 1408 CGCG CG^CG AflII 3060 CTTAAG C^TTAAG Alw44I 177 GTGCAC G^TGCAC AlwNI 1802 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 177 GTGCAC G^TGCAC AspI 3066 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 1972 CACGTG CAC^GTG BcgI 1224 GCAGAATAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 606 TGATCA T^GATCA BfrI 3060 CTTAAG C^TTAAG BglI 1008 GCCTCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsgI 250 GTGCAG GTGCAG(16/14) BsiEI 725 CGGCCG CG[GA][CT]^CG BsmBI 3762 CGTCTC CGTCTC(1/5) BsrBI 1443 GAGCGG CCGCTC(-3/-3) BssSI 3466 CACGAG CACGAG(-5/-1) BstAPI 759 GCAGGCGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 1408 CGCG CG^CG DraIII 2056 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1503 GACAGATGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 725 CGGCCG C^GGCCG EclXI 725 CGGCCG C^GGCCG Eco52I 725 CGGCCG C^GGCCG EcoRV 1252 GATATC GAT^ATC FbaI 606 TGATCA T^GATCA HgaI 745 GCGTC GACGC(5/10) HpaI 1036 GTTAAC GTT^AAC KspI 1407 CCGCGG CCGC^GG MfeI 656 CAATTG C^AATTG MunI 656 CAATTG C^AATTG MvnI 1408 CGCG CG^CG PflFI 3066 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1801 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1972 CACGTG CAC^GTG PmlI 1972 CACGTG CAC^GTG PshAI 1066 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 549 GAGCTC GAGCT^C SacII 1407 CCGCGG CCGC^GG SapI 2746 GAAGAGC GCTCTTC(1/4) ScaI 92 AGTACT AGT^ACT SmaI 3410 CCCGGG CCC^GGG SphI 2591 GCATGC GCATG^C Tth111I 3066 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1801 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 3643 CCATACGTGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 3410 CCCGGG C^CCGGG XmaI 3410 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 43 enzymes) # ################################### AatII 205 GACGTC GACGT^C 2620 GACGTC AccI 193 GTAGAC GT^[AC][GT]AC 3280 GTCTAC AccIII 2154 TCCGGA T^CCGGA 2930 TCCGGA AflIII 183 ACATGT A^C[GA][CT]GT 1173 ACATGT AhdI 1517 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3272 GACTCCTGGTC AocI 1041 CCTGAGG CC^T[ACGT]AGG 1390 CCTAAGG Asp700I 149 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1557 GAACTTATTC AspEI 1517 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3272 GACTCCTGGTC BanII 549 GAGCTC G[GA]GC[CT]^C 1786 GAGCCC BbeI 793 GGCGCC GGCGC^C 1590 GGCGCC BciVI 2322 GGATAC GTATCC(6/5) 3158 GGATAC BlpI 1783 GCTGAGC GC^T[ACGT]AGC 3204 GCTTAGC Bpu1102I 1783 GCTGAGC GC^T[ACGT]AGC 3204 GCTTAGC BsaAI 1972 CACGTG [CT]AC^GT[GA] 3646 TACGTG BseAI 2154 TCCGGA T^CCGGA 2930 TCCGGA Bsp1407I 1413 TGTACA T^GTACA 3301 TGTACA BspEI 2154 TCCGGA T^CCGGA 2930 TCCGGA BspHI 1752 TCATGA T^CATGA 3485 TCATGA BspLU11I 183 ACATGT A^CATGT 1173 ACATGT BspMI 365 GCAGGT ACCTGC(4/8) 2712 GCAGGT BsrFI 333 GCCGGC [GA]^CCGG[CT] 1855 GCCGGC BsrGI 1413 TGTACA T^GTACA 3301 TGTACA Bsu36I 1041 CCTGAGG CC^T[ACGT]AGG 1390 CCTAAGG CelII 1783 GCTGAGC GC^T[ACGT]AGC 3204 GCTTAGC Cfr10I 333 GCCGGC [GA]^CCGG[CT] 1855 GCCGGC Eam1105I 1517 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3272 GACTCCTGGTC Eco81I 1041 CCTGAGG CC^T[ACGT]AGG 1390 CCTAAGG EcoNI 798 CCTGAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2917 CCTTCAGAAGG EcoT22I 1868 ATGCAT ATGCA^T 2534 ATGCAT FauI 28 GCGGG CCCGC(4/6) 1445 GCGGG HindIII 2367 AAGCTT A^AGCTT 3543 AAGCTT KasI 793 GGCGCC G^GCGCC 1590 GGCGCC MroI 2154 TCCGGA T^CCGGA 2930 TCCGGA NaeI 333 GCCGGC GCC^GGC 1855 GCCGGC NarI 793 GGCGCC GG^CGCC 1590 GGCGCC NgoMIV 333 GCCGGC G^CCGGC 1855 GCCGGC NsiI 1868 ATGCAT ATGCA^T 2534 ATGCAT RcaI 1752 TCATGA T^CATGA 3485 TCATGA SfoI 793 GGCGCC GGC^GCC 1590 GGCGCC SmlI 871 CTCAAG C^T[CT][GA]AG 3060 CTTAAG SspBI 1413 TGTACA T^GTACA 3301 TGTACA StuI 2585 AGGCCT AGG^CCT 3674 AGGCCT XmnI 149 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1557 GAACTTATTC ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI ApoI AspHI AvaI AvaII BalI BanI BbsI BbvI BcnI BmyI BpmI BpuAI BsaHI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRI EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII