##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
Acc65I AclI AgeI Aor51HI ApaI
AscI AseI AsnI Asp718I AviII
AvrII BamHI BbvCI BfaI BglII
BlnI BsaBI BsaI BsiWI BspDI
BsrDI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
Eco47III EcoO65I FseI FspI KpnI
MaeI MamI MluI NdeI NheI
NotI NruI NspV PacI PaeR7I
PinAI PmeI PshBI Psp1406I PvuI
RsrII SalI SbfI SexAI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccII 1408 CGCG CG^CG
AflII 3060 CTTAAG C^TTAAG
Alw44I 177 GTGCAC G^TGCAC
AlwNI 1802 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 177 GTGCAC G^TGCAC
AspI 3066 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1972 CACGTG CAC^GTG
BcgI 1224 GCAGAATAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 606 TGATCA T^GATCA
BfrI 3060 CTTAAG C^TTAAG
BglI 1008 GCCTCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 250 GTGCAG GTGCAG(16/14)
BsiEI 725 CGGCCG CG[GA][CT]^CG
BsmBI 3762 CGTCTC CGTCTC(1/5)
BsrBI 1443 GAGCGG CCGCTC(-3/-3)
BssSI 3466 CACGAG CACGAG(-5/-1)
BstAPI 759 GCAGGCGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1408 CGCG CG^CG
DraIII 2056 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1503 GACAGATGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 725 CGGCCG C^GGCCG
EclXI 725 CGGCCG C^GGCCG
Eco52I 725 CGGCCG C^GGCCG
EcoRV 1252 GATATC GAT^ATC
FbaI 606 TGATCA T^GATCA
HgaI 745 GCGTC GACGC(5/10)
HpaI 1036 GTTAAC GTT^AAC
KspI 1407 CCGCGG CCGC^GG
MfeI 656 CAATTG C^AATTG
MunI 656 CAATTG C^AATTG
MvnI 1408 CGCG CG^CG
PflFI 3066 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1801 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1972 CACGTG CAC^GTG
PmlI 1972 CACGTG CAC^GTG
PshAI 1066 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 549 GAGCTC GAGCT^C
SacII 1407 CCGCGG CCGC^GG
SapI 2746 GAAGAGC GCTCTTC(1/4)
ScaI 92 AGTACT AGT^ACT
SmaI 3410 CCCGGG CCC^GGG
SphI 2591 GCATGC GCATG^C
Tth111I 3066 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1801 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 3643 CCATACGTGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 3410 CCCGGG C^CCGGG
XmaI 3410 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 43 enzymes) #
###################################
AatII 205 GACGTC GACGT^C
2620 GACGTC
AccI 193 GTAGAC GT^[AC][GT]AC
3280 GTCTAC
AccIII 2154 TCCGGA T^CCGGA
2930 TCCGGA
AflIII 183 ACATGT A^C[GA][CT]GT
1173 ACATGT
AhdI 1517 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3272 GACTCCTGGTC
AocI 1041 CCTGAGG CC^T[ACGT]AGG
1390 CCTAAGG
Asp700I 149 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1557 GAACTTATTC
AspEI 1517 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3272 GACTCCTGGTC
BanII 549 GAGCTC G[GA]GC[CT]^C
1786 GAGCCC
BbeI 793 GGCGCC GGCGC^C
1590 GGCGCC
BciVI 2322 GGATAC GTATCC(6/5)
3158 GGATAC
BlpI 1783 GCTGAGC GC^T[ACGT]AGC
3204 GCTTAGC
Bpu1102I 1783 GCTGAGC GC^T[ACGT]AGC
3204 GCTTAGC
BsaAI 1972 CACGTG [CT]AC^GT[GA]
3646 TACGTG
BseAI 2154 TCCGGA T^CCGGA
2930 TCCGGA
Bsp1407I 1413 TGTACA T^GTACA
3301 TGTACA
BspEI 2154 TCCGGA T^CCGGA
2930 TCCGGA
BspHI 1752 TCATGA T^CATGA
3485 TCATGA
BspLU11I 183 ACATGT A^CATGT
1173 ACATGT
BspMI 365 GCAGGT ACCTGC(4/8)
2712 GCAGGT
BsrFI 333 GCCGGC [GA]^CCGG[CT]
1855 GCCGGC
BsrGI 1413 TGTACA T^GTACA
3301 TGTACA
Bsu36I 1041 CCTGAGG CC^T[ACGT]AGG
1390 CCTAAGG
CelII 1783 GCTGAGC GC^T[ACGT]AGC
3204 GCTTAGC
Cfr10I 333 GCCGGC [GA]^CCGG[CT]
1855 GCCGGC
Eam1105I 1517 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3272 GACTCCTGGTC
Eco81I 1041 CCTGAGG CC^T[ACGT]AGG
1390 CCTAAGG
EcoNI 798 CCTGAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2917 CCTTCAGAAGG
EcoT22I 1868 ATGCAT ATGCA^T
2534 ATGCAT
FauI 28 GCGGG CCCGC(4/6)
1445 GCGGG
HindIII 2367 AAGCTT A^AGCTT
3543 AAGCTT
KasI 793 GGCGCC G^GCGCC
1590 GGCGCC
MroI 2154 TCCGGA T^CCGGA
2930 TCCGGA
NaeI 333 GCCGGC GCC^GGC
1855 GCCGGC
NarI 793 GGCGCC GG^CGCC
1590 GGCGCC
NgoMIV 333 GCCGGC G^CCGGC
1855 GCCGGC
NsiI 1868 ATGCAT ATGCA^T
2534 ATGCAT
RcaI 1752 TCATGA T^CATGA
3485 TCATGA
SfoI 793 GGCGCC GGC^GCC
1590 GGCGCC
SmlI 871 CTCAAG C^T[CT][GA]AG
3060 CTTAAG
SspBI 1413 TGTACA T^GTACA
3301 TGTACA
StuI 2585 AGGCCT AGG^CCT
3674 AGGCCT
XmnI 149 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1557 GAACTTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI AspHI AvaI AvaII
BalI BanI BbsI BbvI BcnI
BmyI BpmI BpuAI BsaHI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII