##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AgeI Aor51HI AscI Asp700I Asp718I
AspI AviII BalI BanI BbeI
BbrPI BcgI BclI BfrI BlpI
Bpu1102I BsiEI BsiWI BsmBI Bsp1407I
BspDI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I CelII Cfr10I ClaI
CpoI EaeI EagI EclXI Eco47III
Eco52I EcoO65I EcoRI EcoT22I FauI
FbaI FseI FspI HaeII KasI
KpnI KspI MfeI MluI MluNI
MscI MunI MvnI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tth111I Van91I
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 339 GTCTAC GT^[AC][GT]AC
AccIII 1175 TCCGGA T^CCGGA
AciI 2015 GCGG CCGC(-3/-1)
AcyI 2532 GGCGTC G[GA]^CG[CT]C
AhdI 2925 GACTATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 867 GTGCAC G^TGCAC
AlwNI 549 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 536 CCTGAGG CC^T[ACGT]AGG
ApaI 574 GGGCCC GGGCC^C
ApaLI 867 GTGCAC G^TGCAC
AseI 2785 ATTAAT AT^TAAT
AsnI 2785 ATTAAT AT^TAAT
AspEI 2925 GACTATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1459 CCCGGG C^[CT]CG[GA]G
AvrII 1983 CCTAGG C^CTAGG
BamHI 1836 GGATCC G^GATCC
BbvCI 2344 CCTCAGC CCTCAGC(-5/-2)
BciVI 1199 GGATAC GTATCC(6/5)
BglI 981 GCCTCAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1983 CCTAGG C^CTAGG
BsaAI 2068 TACGTG [CT]AC^GT[GA]
BsaHI 2532 GGCGTC G[GA]^CG[CT]C
BsaWI 1175 TCCGGA [AT]^CCGG[AT]
BseAI 1175 TCCGGA T^CCGGA
BsmI 2213 GAATGC GAATGC(1/-1)
BsoBI 1459 CCCGGG C^[CT]CG[GA]G
BspEI 1175 TCCGGA T^CCGGA
BspHI 2447 TCATGA T^CATGA
Bsu36I 536 CCTGAGG CC^T[ACGT]AGG
CfoI 581 GCGC GCG^C
DraIII 1971 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 157 CCATGG C^C[GA][CT]GG
Eam1105I 2925 GACTATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 536 CCTGAGG CC^T[ACGT]AGG
EcoNI 428 CCTCTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HhaI 581 GCGC GCG^C
Hin1I 2532 GGCGTC G[GA]^CG[CT]C
HinP1I 581 GCGC G^CGC
HindIII 65 AAGCTT A^AGCTT
HpaI 2623 GTTAAC GTT^AAC
MroI 1175 TCCGGA T^CCGGA
MspA1I 2226 CAGCTG C[AC]G^C[GT]G
NcoI 157 CCATGG C^CATGG
PpuMI 2338 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 2785 ATTAAT AT^TAAT
PvuII 2226 CAGCTG CAG^CTG
RcaI 2447 TCATGA T^CATGA
SacI 931 GAGCTC GAGCT^C
SmaI 1459 CCCGGG CCC^GGG
XmaCI 1459 CCCGGG C^CCGGG
XmaI 1459 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BglII 95 AGATCT A^GATCT
2741 AGATCT
BsaBI 1147 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1874 GATGAACATC
BsaI 2700 GAGACC GGTCTC(1/5)
1525 GGTCTC
BseRI 2899 CTCCTC GAGGAG(10/8)
282 GAGGAG
BspLU11I 2541 ACATGT A^CATGT
2640 ACATGT
BstXI 2281 CCAAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2356 CCACAGTGCTGG
DrdI 330 GACTCTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2575 GACCACTGAGTC
EarI 459 GAAGAG CTCTTC(1/4)
1109 GAAGAG
HgaI 1212 GACGC GACGC(5/10)
2533 GCGTC
HincII 2623 GTTAAC GT[CT]^[GA]AC
2838 GTCAAC
HindII 2623 GTTAAC GT[CT]^[GA]AC
2838 GTCAAC
Ksp632I 459 GAAGAG CTCTTC(1/4)
1109 GAAGAG
MaeII 496 ACGT A^CGT
2069 ACGT
MamI 1147 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1874 GATGAACATC
NdeI 2080 CATATG CA^TATG
2673 CATATG
ScaI 2914 AGTACT AGT^ACT
3120 AGTACT
SphI 2381 GCATGC GCATG^C
2754 GCATGC
StuI 988 AGGCCT AGG^CCT
1846 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AcsI AfaI AflIII AluI AlwI
ApoI AspHI AvaII BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII EcoO109I EcoRII
EcoRV EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII