################################## # NO CUT ENZYMES ( 103 enzymes) # ################################## AatII Acc65I AccII AclI AflII AgeI Aor51HI AscI Asp700I Asp718I AspI AviII BalI BanI BbeI BbrPI BcgI BclI BfrI BlpI Bpu1102I BsiEI BsiWI BsmBI Bsp1407I BspDI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I CelII Cfr10I ClaI CpoI EaeI EagI EclXI Eco47III Eco52I EcoO65I EcoRI EcoT22I FauI FbaI FseI FspI HaeII KasI KpnI KspI MfeI MluI MluNI MscI MunI MvnI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccI 339 GTCTAC GT^[AC][GT]AC AccIII 1175 TCCGGA T^CCGGA AciI 2015 GCGG CCGC(-3/-1) AcyI 2532 GGCGTC G[GA]^CG[CT]C AhdI 2925 GACTATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 867 GTGCAC G^TGCAC AlwNI 549 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 536 CCTGAGG CC^T[ACGT]AGG ApaI 574 GGGCCC GGGCC^C ApaLI 867 GTGCAC G^TGCAC AseI 2785 ATTAAT AT^TAAT AsnI 2785 ATTAAT AT^TAAT AspEI 2925 GACTATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 1459 CCCGGG C^[CT]CG[GA]G AvrII 1983 CCTAGG C^CTAGG BamHI 1836 GGATCC G^GATCC BbvCI 2344 CCTCAGC CCTCAGC(-5/-2) BciVI 1199 GGATAC GTATCC(6/5) BglI 981 GCCTCAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 1983 CCTAGG C^CTAGG BsaAI 2068 TACGTG [CT]AC^GT[GA] BsaHI 2532 GGCGTC G[GA]^CG[CT]C BsaWI 1175 TCCGGA [AT]^CCGG[AT] BseAI 1175 TCCGGA T^CCGGA BsmI 2213 GAATGC GAATGC(1/-1) BsoBI 1459 CCCGGG C^[CT]CG[GA]G BspEI 1175 TCCGGA T^CCGGA BspHI 2447 TCATGA T^CATGA Bsu36I 536 CCTGAGG CC^T[ACGT]AGG CfoI 581 GCGC GCG^C DraIII 1971 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 157 CCATGG C^C[GA][CT]GG Eam1105I 2925 GACTATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 536 CCTGAGG CC^T[ACGT]AGG EcoNI 428 CCTCTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HhaI 581 GCGC GCG^C Hin1I 2532 GGCGTC G[GA]^CG[CT]C HinP1I 581 GCGC G^CGC HindIII 65 AAGCTT A^AGCTT HpaI 2623 GTTAAC GTT^AAC MroI 1175 TCCGGA T^CCGGA MspA1I 2226 CAGCTG C[AC]G^C[GT]G NcoI 157 CCATGG C^CATGG PpuMI 2338 AGGACCC [GA]G^G[AT]CC[CT] PshBI 2785 ATTAAT AT^TAAT PvuII 2226 CAGCTG CAG^CTG RcaI 2447 TCATGA T^CATGA SacI 931 GAGCTC GAGCT^C SmaI 1459 CCCGGG CCC^GGG XmaCI 1459 CCCGGG C^CCGGG XmaI 1459 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### BglII 95 AGATCT A^GATCT 2741 AGATCT BsaBI 1147 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1874 GATGAACATC BsaI 2700 GAGACC GGTCTC(1/5) 1525 GGTCTC BseRI 2899 CTCCTC GAGGAG(10/8) 282 GAGGAG BspLU11I 2541 ACATGT A^CATGT 2640 ACATGT BstXI 2281 CCAAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2356 CCACAGTGCTGG DrdI 330 GACTCTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2575 GACCACTGAGTC EarI 459 GAAGAG CTCTTC(1/4) 1109 GAAGAG HgaI 1212 GACGC GACGC(5/10) 2533 GCGTC HincII 2623 GTTAAC GT[CT]^[GA]AC 2838 GTCAAC HindII 2623 GTTAAC GT[CT]^[GA]AC 2838 GTCAAC Ksp632I 459 GAAGAG CTCTTC(1/4) 1109 GAAGAG MaeII 496 ACGT A^CGT 2069 ACGT MamI 1147 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1874 GATGAACATC NdeI 2080 CATATG CA^TATG 2673 CATATG ScaI 2914 AGTACT AGT^ACT 3120 AGTACT SphI 2381 GCATGC GCATG^C 2754 GCATGC StuI 988 AGGCCT AGG^CCT 1846 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 86 enzymes) # ######################################### AcsI AfaI AflIII AluI AlwI ApoI AspHI AvaII BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII EcoO109I EcoRII EcoRV EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII