##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII AccII AclI AcyI AflII
AgeI AscI AseI AsnI AspI
AviII BbeI BbrPI BcgI BclI
BfrI BsaHI BsiEI BsiWI Bsp1407I
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstZ17I Cfr10I
CpoI DraI EagI EclXI Eco52I
EcoNI EcoRV EcoT22I FbaI FseI
FspI Hin1I HpaI KasI KspI
MfeI MluI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacII SbfI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SwaI Tth111I
XbaI
###################################
# ONE CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 1629 GGTACC G^GTACC
Alw44I 2246 GTGCAC G^TGCAC
Aor51HI 2889 AGCGCT AGC^GCT
ApaLI 2246 GTGCAC G^TGCAC
Asp718I 1629 GGTACC G^GTACC
BamHI 400 GGATCC G^GATCC
BbvCI 934 CCTCAGC CCTCAGC(-5/-2)
BglII 315 AGATCT A^GATCT
BsmI 4526 GAATGC GAATGC(1/-1)
BspHI 177 TCATGA T^CATGA
BspLU11I 4033 ACATGT A^CATGT
BsrBI 2308 CCGCTC CCGCTC(-3/-3)
Eco47III 2889 AGCGCT AGC^GCT
HaeII 2889 AGCGCT [GA]GCGC^[CT]
KpnI 1629 GGTACC GGTAC^C
NcoI 4463 CCATGG C^CATGG
PshAI 2483 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 177 TCATGA T^CATGA
SalI 1034 GTCGAC G^TCGAC
SapI 907 GAAGAGC GCTCTTC(1/4)
ScaI 2697 AGTACT AGT^ACT
SexAI 2214 ACCTGGT A^CC[AT]GGT
SmaI 1362 CCCGGG CCC^GGG
SphI 2267 GCATGC GCATG^C
SspI 3147 AATATT AAT^ATT
XmaCI 1362 CCCGGG C^CCGGG
XmaI 1362 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccIII 447 TCCGGA T^CCGGA
2790 TCCGGA
AhdI 537 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3290 GACTGCTGGTC
ApaI 2072 GGGCCC GGGCC^C
4344 GGGCCC
AspEI 537 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3290 GACTGCTGGTC
BalI 1841 TGGCCA TGG^CCA
3516 TGGCCA
BciVI 2674 GGATAC GTATCC(6/5)
3690 GGATAC
BsaAI 95 TACGTG [CT]AC^GT[GA]
2237 TACGTG
BseAI 447 TCCGGA T^CCGGA
2790 TCCGGA
BsmBI 1676 GAGACG CGTCTC(1/5)
3104 GAGACG
BspDI 1527 ATCGAT AT^CGAT
2762 ATCGAT
BspEI 447 TCCGGA T^CCGGA
2790 TCCGGA
BstEII 2581 GGTCACC G^GT[ACGT]ACC
4467 GGTTACC
BstPI 2581 GGTCACC G^GT[ACGT]ACC
4467 GGTTACC
ClaI 1527 ATCGAT AT^CGAT
2762 ATCGAT
DraIII 1775 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
3062 CACATTGTG
DrdI 1850 GACACAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1868 GACATTAAAGTC
Eam1105I 537 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3290 GACTGCTGGTC
EcoO65I 2581 GGTCACC G^GT[ACGT]ACC
4467 GGTTACC
EcoRI 1003 GAATTC G^AATTC
4586 GAATTC
HincII 1034 GTCGAC GT[CT]^[GA]AC
2210 GTCAAC
HindII 1034 GTCGAC GT[CT]^[GA]AC
2210 GTCAAC
HindIII 3713 AAGCTT A^AGCTT
4010 AAGCTT
MluNI 1841 TGGCCA TGG^CCA
3516 TGGCCA
MroI 447 TCCGGA T^CCGGA
2790 TCCGGA
MscI 1841 TGGCCA TGG^CCA
3516 TGGCCA
NspI 2267 GCATGC [GA]CATG^[CT]
4033 ACATGT
PflMI 2063 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2813 CCACAGGATGG
StuI 1461 AGGCCT AGG^CCT
3774 AGGCCT
Van91I 2063 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2813 CCACAGGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI AvaI AvaII AvrII
BanI BanII BbsI BbvI BcnI
BfaI BglI BlnI BlpI BmyI
BpmI Bpu1102I BpuAI BsaBI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PaeR7I PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoI XhoII
XmnI