################################## # NO CUT ENZYMES ( 81 enzymes) # ################################## AatII AccII AclI AcyI AflII AgeI AscI AseI AsnI AspI AviII BbeI BbrPI BcgI BclI BfrI BsaHI BsiEI BsiWI Bsp1407I BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Cfr10I CpoI DraI EagI EclXI Eco52I EcoNI EcoRV EcoT22I FbaI FseI FspI Hin1I HpaI KasI KspI MfeI MluI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SbfI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 27 enzymes) # ################################### Acc65I 1629 GGTACC G^GTACC Alw44I 2246 GTGCAC G^TGCAC Aor51HI 2889 AGCGCT AGC^GCT ApaLI 2246 GTGCAC G^TGCAC Asp718I 1629 GGTACC G^GTACC BamHI 400 GGATCC G^GATCC BbvCI 934 CCTCAGC CCTCAGC(-5/-2) BglII 315 AGATCT A^GATCT BsmI 4526 GAATGC GAATGC(1/-1) BspHI 177 TCATGA T^CATGA BspLU11I 4033 ACATGT A^CATGT BsrBI 2308 CCGCTC CCGCTC(-3/-3) Eco47III 2889 AGCGCT AGC^GCT HaeII 2889 AGCGCT [GA]GCGC^[CT] KpnI 1629 GGTACC GGTAC^C NcoI 4463 CCATGG C^CATGG PshAI 2483 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 177 TCATGA T^CATGA SalI 1034 GTCGAC G^TCGAC SapI 907 GAAGAGC GCTCTTC(1/4) ScaI 2697 AGTACT AGT^ACT SexAI 2214 ACCTGGT A^CC[AT]GGT SmaI 1362 CCCGGG CCC^GGG SphI 2267 GCATGC GCATG^C SspI 3147 AATATT AAT^ATT XmaCI 1362 CCCGGG C^CCGGG XmaI 1362 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccIII 447 TCCGGA T^CCGGA 2790 TCCGGA AhdI 537 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3290 GACTGCTGGTC ApaI 2072 GGGCCC GGGCC^C 4344 GGGCCC AspEI 537 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3290 GACTGCTGGTC BalI 1841 TGGCCA TGG^CCA 3516 TGGCCA BciVI 2674 GGATAC GTATCC(6/5) 3690 GGATAC BsaAI 95 TACGTG [CT]AC^GT[GA] 2237 TACGTG BseAI 447 TCCGGA T^CCGGA 2790 TCCGGA BsmBI 1676 GAGACG CGTCTC(1/5) 3104 GAGACG BspDI 1527 ATCGAT AT^CGAT 2762 ATCGAT BspEI 447 TCCGGA T^CCGGA 2790 TCCGGA BstEII 2581 GGTCACC G^GT[ACGT]ACC 4467 GGTTACC BstPI 2581 GGTCACC G^GT[ACGT]ACC 4467 GGTTACC ClaI 1527 ATCGAT AT^CGAT 2762 ATCGAT DraIII 1775 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG 3062 CACATTGTG DrdI 1850 GACACAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1868 GACATTAAAGTC Eam1105I 537 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3290 GACTGCTGGTC EcoO65I 2581 GGTCACC G^GT[ACGT]ACC 4467 GGTTACC EcoRI 1003 GAATTC G^AATTC 4586 GAATTC HincII 1034 GTCGAC GT[CT]^[GA]AC 2210 GTCAAC HindII 1034 GTCGAC GT[CT]^[GA]AC 2210 GTCAAC HindIII 3713 AAGCTT A^AGCTT 4010 AAGCTT MluNI 1841 TGGCCA TGG^CCA 3516 TGGCCA MroI 447 TCCGGA T^CCGGA 2790 TCCGGA MscI 1841 TGGCCA TGG^CCA 3516 TGGCCA NspI 2267 GCATGC [GA]CATG^[CT] 4033 ACATGT PflMI 2063 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2813 CCACAGGATGG StuI 1461 AGGCCT AGG^CCT 3774 AGGCCT Van91I 2063 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2813 CCACAGGATGG ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI Asp700I AspHI AvaI AvaII AvrII BanI BanII BbsI BbvI BcnI BfaI BglI BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaBI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PaeR7I PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoI XhoII XmnI