################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## Acc65I AccIII AclI AflII Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AviII AvrII BbeI BbvCI BcgI BfrI BlnI BlpI Bpu1102I BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BsrGI BssHII BstBI Bsu36I CelII ClaI CpoI EagI EclXI Eco47III Eco52I Eco81I EcoRV FseI FspI HaeII HindIII HpaI KasI KpnI KspI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AatII 511 GACGTC GACGT^C AccI 3539 GTATAC GT^[AC][GT]AC AccII 4378 CGCG CG^CG AgeI 3863 ACCGGT A^CCGGT ApaI 2996 GGGCCC GGGCC^C AspI 2430 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 737 GGATCC G^GATCC BanI 4036 GGTGCC G^G[CT][GA]CC BpmI 609 CTGGAG CTGGAG(16/14) BsaBI 1479 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BspHI 2073 TCATGA T^CATGA BspLU11I 3507 ACATGT A^CATGT Bst1107I 3539 GTATAC GTA^TAC BstAPI 858 GCAATGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 4378 CGCG CG^CG BstXI 4350 CCATCACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 3539 GTATAC GTA^TAC CfoI 1586 GCGC GCG^C EcoRI 1948 GAATTC G^AATTC EcoT22I 3348 ATGCAT ATGCA^T HgaI 2390 GCGTC GACGC(5/10) HhaI 1586 GCGC GCG^C HinP1I 1586 GCGC G^CGC MamI 1479 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MvnI 4378 CGCG CG^CG NsiI 3348 ATGCAT ATGCA^T PaeR7I 3332 CTCGAG C^TCGAG PflFI 2430 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 3863 ACCGGT A^CCGGT PshAI 3030 GACCAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 3082 CTGCAG CTGCA^G RcaI 2073 TCATGA T^CATGA SexAI 2376 ACCTGGT A^CC[AT]GGT SmaI 2010 CCCGGG CCC^GGG StuI 1568 AGGCCT AGG^CCT Tth111I 2430 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 3332 CTCGAG C^TCGAG XmaCI 2010 CCCGGG C^CCGGG XmaI 2010 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AcyI 511 GACGTC G[GA]^CG[CT]C 2389 GGCGTC AflIII 3507 ACATGT A^C[GA][CT]GT 3749 ACGTGT BbrPI 2394 CACGTG CAC^GTG 3748 CACGTG BciVI 4136 GGATAC GTATCC(6/5) 245 GTATCC BclI 1060 TGATCA T^GATCA 1484 TGATCA BglI 637 GCCATGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2807 GCCAGTGGGGC BsaAI 2394 CACGTG [CT]AC^GT[GA] 3748 CACGTG BsaHI 511 GACGTC G[GA]^CG[CT]C 2389 GGCGTC BsaWI 3863 ACCGGT [AT]^CCGG[AT] 3972 ACCGGA BsmBI 509 GAGACG CGTCTC(1/5) 2177 GAGACG BsrBI 4441 CCGCTC CCGCTC(-3/-3) 1297 GAGCGG BsrDI 858 GCAATG GCAATG(2/0) 1005 GCAATG BsrFI 1557 GCCGGT [GA]^CCGG[CT] 3863 ACCGGT BstEII 3036 GGTCACC G^GT[ACGT]ACC 3968 GGTTACC BstPI 3036 GGTCACC G^GT[ACGT]ACC 3968 GGTTACC Cfr10I 1557 GCCGGT [GA]^CCGG[CT] 3863 ACCGGT DraI 2684 TTTAAA TTT^AAA 4707 TTTAAA DrdI 2077 GACCGGGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3302 GACTGGCTGGTC EcoNI 784 CCTGGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1787 CCTTACACAGG EcoO65I 3036 GGTCACC G^GT[ACGT]ACC 3968 GGTTACC FbaI 1060 TGATCA T^GATCA 1484 TGATCA Hin1I 511 GACGTC G[GA]^CG[CT]C 2389 GGCGTC NcoI 3390 CCATGG C^CATGG 4736 CCATGG PmaCI 2394 CACGTG CAC^GTG 3748 CACGTG PmlI 2394 CACGTG CAC^GTG 3748 CACGTG PpuMI 531 AGGTCCT [GA]G^G[AT]CC[CT] 4058 AGGACCC PvuII 577 CAGCTG CAG^CTG 1353 CAGCTG SacI 3962 GAGCTC GAGCT^C 4385 GAGCTC XbaI 1222 TCTAGA T^CTAGA 4542 TCTAGA XcmI 2538 CCATTTACACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2906 CCAAGTCTGTAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 107 enzymes) # ######################################### AciI AcsI AfaI AhdI AluI AlwI AlwNI ApoI Asp700I AspEI AspHI AvaI AvaII BalI BanII BbsI BbvI BcnI BfaI BglII BmyI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BssSI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI Eam1105I EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI