##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
Acc65I AccIII AclI AflII Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AviII AvrII BbeI
BbvCI BcgI BfrI BlnI BlpI
Bpu1102I BseAI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BsrGI BssHII
BstBI Bsu36I CelII ClaI CpoI
EagI EclXI Eco47III Eco52I Eco81I
EcoRV FseI FspI HaeII HindIII
HpaI KasI KpnI KspI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PmeI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 511 GACGTC GACGT^C
AccI 3539 GTATAC GT^[AC][GT]AC
AccII 4378 CGCG CG^CG
AgeI 3863 ACCGGT A^CCGGT
ApaI 2996 GGGCCC GGGCC^C
AspI 2430 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 737 GGATCC G^GATCC
BanI 4036 GGTGCC G^G[CT][GA]CC
BpmI 609 CTGGAG CTGGAG(16/14)
BsaBI 1479 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 2073 TCATGA T^CATGA
BspLU11I 3507 ACATGT A^CATGT
Bst1107I 3539 GTATAC GTA^TAC
BstAPI 858 GCAATGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 4378 CGCG CG^CG
BstXI 4350 CCATCACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3539 GTATAC GTA^TAC
CfoI 1586 GCGC GCG^C
EcoRI 1948 GAATTC G^AATTC
EcoT22I 3348 ATGCAT ATGCA^T
HgaI 2390 GCGTC GACGC(5/10)
HhaI 1586 GCGC GCG^C
HinP1I 1586 GCGC G^CGC
MamI 1479 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 4378 CGCG CG^CG
NsiI 3348 ATGCAT ATGCA^T
PaeR7I 3332 CTCGAG C^TCGAG
PflFI 2430 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3863 ACCGGT A^CCGGT
PshAI 3030 GACCAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 3082 CTGCAG CTGCA^G
RcaI 2073 TCATGA T^CATGA
SexAI 2376 ACCTGGT A^CC[AT]GGT
SmaI 2010 CCCGGG CCC^GGG
StuI 1568 AGGCCT AGG^CCT
Tth111I 2430 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3332 CTCGAG C^TCGAG
XmaCI 2010 CCCGGG C^CCGGG
XmaI 2010 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 511 GACGTC G[GA]^CG[CT]C
2389 GGCGTC
AflIII 3507 ACATGT A^C[GA][CT]GT
3749 ACGTGT
BbrPI 2394 CACGTG CAC^GTG
3748 CACGTG
BciVI 4136 GGATAC GTATCC(6/5)
245 GTATCC
BclI 1060 TGATCA T^GATCA
1484 TGATCA
BglI 637 GCCATGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2807 GCCAGTGGGGC
BsaAI 2394 CACGTG [CT]AC^GT[GA]
3748 CACGTG
BsaHI 511 GACGTC G[GA]^CG[CT]C
2389 GGCGTC
BsaWI 3863 ACCGGT [AT]^CCGG[AT]
3972 ACCGGA
BsmBI 509 GAGACG CGTCTC(1/5)
2177 GAGACG
BsrBI 4441 CCGCTC CCGCTC(-3/-3)
1297 GAGCGG
BsrDI 858 GCAATG GCAATG(2/0)
1005 GCAATG
BsrFI 1557 GCCGGT [GA]^CCGG[CT]
3863 ACCGGT
BstEII 3036 GGTCACC G^GT[ACGT]ACC
3968 GGTTACC
BstPI 3036 GGTCACC G^GT[ACGT]ACC
3968 GGTTACC
Cfr10I 1557 GCCGGT [GA]^CCGG[CT]
3863 ACCGGT
DraI 2684 TTTAAA TTT^AAA
4707 TTTAAA
DrdI 2077 GACCGGGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3302 GACTGGCTGGTC
EcoNI 784 CCTGGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1787 CCTTACACAGG
EcoO65I 3036 GGTCACC G^GT[ACGT]ACC
3968 GGTTACC
FbaI 1060 TGATCA T^GATCA
1484 TGATCA
Hin1I 511 GACGTC G[GA]^CG[CT]C
2389 GGCGTC
NcoI 3390 CCATGG C^CATGG
4736 CCATGG
PmaCI 2394 CACGTG CAC^GTG
3748 CACGTG
PmlI 2394 CACGTG CAC^GTG
3748 CACGTG
PpuMI 531 AGGTCCT [GA]G^G[AT]CC[CT]
4058 AGGACCC
PvuII 577 CAGCTG CAG^CTG
1353 CAGCTG
SacI 3962 GAGCTC GAGCT^C
4385 GAGCTC
XbaI 1222 TCTAGA T^CTAGA
4542 TCTAGA
XcmI 2538 CCATTTACACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2906 CCAAGTCTGTAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AciI AcsI AfaI AhdI AluI
AlwI AlwNI ApoI Asp700I AspEI
AspHI AvaI AvaII BalI BanII
BbsI BbvI BcnI BfaI BglII
BmyI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BssSI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI Eam1105I EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI