##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AgeI AhdI ApaI
AscI Asp718I AspEI AspI AvrII
BamHI BbrPI BcgI BciVI BclI
BglI BlnI BlpI Bpu1102I BsiEI
BsiWI BsmBI BspMI BsrBI BstEII
BstPI CelII CpoI EagI Eam1105I
EclXI Eco52I EcoNI EcoO65I EcoRI
FbaI FseI HpaI KpnI KspI
MfeI MluI MunI NdeI NheI
NotI NruI PacI PflFI PinAI
PmaCI PmeI PmlI PvuI RsrII
SacII SalI SapI SbfI SexAI
SfiI SgrAI SnaBI SpeI SphI
Sse8387I StuI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AccIII 189 TCCGGA T^CCGGA
AclI 2331 AACGTT AA^CGTT
AflII 2162 CTTAAG C^TTAAG
Alw44I 2889 GTGCAC G^TGCAC
AocI 3124 CCTTAGG CC^T[ACGT]AGG
Aor51HI 208 AGCGCT AGC^GCT
ApaLI 2889 GTGCAC G^TGCAC
AseI 1674 ATTAAT AT^TAAT
AsnI 1674 ATTAAT AT^TAAT
BbeI 2878 GGCGCC GGCGC^C
BbvCI 71 GCTGAGG CCTCAGC(-5/-2)
BfrI 2162 CTTAAG C^TTAAG
BsaAI 2141 TACGTG [CT]AC^GT[GA]
BsaWI 189 TCCGGA [AT]^CCGG[AT]
BseAI 189 TCCGGA T^CCGGA
BspDI 3159 ATCGAT AT^CGAT
BspEI 189 TCCGGA T^CCGGA
BspHI 3716 TCATGA T^CATGA
BsrDI 1847 GCAATG GCAATG(2/0)
BssHII 146 GCGCGC G^CGCGC
BssSI 3687 CACGAG CACGAG(-5/-1)
Bst1107I 1021 GTATAC GTA^TAC
BstAPI 3660 GCATTAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3466 TTCGAA TT^CGAA
BstZ17I 1021 GTATAC GTA^TAC
Bsu36I 3124 CCTTAGG CC^T[ACGT]AGG
ClaI 3159 ATCGAT AT^CGAT
DraIII 1064 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 341 GACAGCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 28 CCATGG C^C[GA][CT]GG
Eco47III 208 AGCGCT AGC^GCT
Eco81I 3124 CCTTAGG CC^T[ACGT]AGG
EcoRV 2841 GATATC GAT^ATC
EcoT14I 28 CCATGG C^C[AT][AT]GG
FauI 46 GCGGG CCCGC(4/6)
HindIII 1079 AAGCTT A^AGCTT
KasI 2878 GGCGCC G^GCGCC
MroI 189 TCCGGA T^CCGGA
NaeI 4152 GCCGGC GCC^GGC
NarI 2878 GGCGCC GG^CGCC
NcoI 28 CCATGG C^CATGG
NgoMIV 4152 GCCGGC G^CCGGC
NspV 3466 TTCGAA TT^CGAA
PaeR7I 135 CTCGAG C^TCGAG
PflMI 1819 CCAGAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 3277 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1674 ATTAAT AT^TAAT
Psp1406I 2331 AACGTT AA^CGTT
RcaI 3716 TCATGA T^CATGA
SacI 132 GAGCTC GAGCT^C
SfoI 2878 GGCGCC GGC^GCC
SfuI 3466 TTCGAA TT^CGAA
SmaI 825 CCCGGG CCC^GGG
StyI 28 CCATGG C^C[AT][AT]GG
Van91I 1819 CCAGAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 4564 CCAGTATGTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 135 CTCGAG C^TCGAG
XmaCI 825 CCCGGG C^CCGGG
XmaI 825 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 734 GTCTAC GT^[AC][GT]AC
1021 GTATAC
AccII 147 CGCG CG^CG
149 CGCG
AcyI 759 GACGCC G[GA]^CG[CT]C
2878 GGCGCC
AlwNI 3829 CAGTAACTG CAG[ACGT][ACGT][ACGT]^CTG
3904 CAGAGTCTG
AviII 3598 TGCGCA TGC^GCA
4136 TGCGCA
BanI 2878 GGCGCC G^G[CT][GA]CC
4076 GGTGCC
BcnI 825 CCCGG CC^[GC]GG
826 CCGGG
BglII 1913 AGATCT A^GATCT
4323 AGATCT
BsaHI 759 GACGCC G[GA]^CG[CT]C
2878 GGCGCC
BsmI 2361 GAATGC GAATGC(1/-1)
1794 GCATTC
Bsp1407I 2689 TGTACA T^GTACA
2863 TGTACA
BsrFI 3316 GCCGGT [GA]^CCGG[CT]
4152 GCCGGC
BsrGI 2689 TGTACA T^GTACA
2863 TGTACA
BstUI 147 CGCG CG^CG
149 CGCG
BstXI 1836 CCACAGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1968 CCATACACATGG
Cfr10I 3316 GCCGGT [GA]^CCGG[CT]
4152 GCCGGC
EcoT22I 991 ATGCAT ATGCA^T
3148 ATGCAT
FspI 3598 TGCGCA TGC^GCA
4136 TGCGCA
HgaI 759 GACGC GACGC(5/10)
4337 GCGTC
Hin1I 759 GACGCC G[GA]^CG[CT]C
2878 GGCGCC
MvnI 147 CGCG CG^CG
149 CGCG
NciI 825 CCCGG CC^[GC]GG
826 CCGGG
NsiI 991 ATGCAT ATGCA^T
3148 ATGCAT
PpuMI 94 AGGTCCC [GA]G^G[AT]CC[CT]
219 AGGACCT
ScaI 3047 AGTACT AGT^ACT
4371 AGTACT
SspBI 2689 TGTACA T^GTACA
2863 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI Asp700I AspHI AvaI
AvaII BalI BanII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaBI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspLU11I BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EaeI
EarI EcoO109I EcoRII Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI