##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AhdI Alw44I Aor51HI ApaI
ApaLI AscI Asp718I AspEI AvrII
BbeI BbrPI BglI BlnI BsaHI
BseAI BsiWI BsmBI BspDI BspEI
BspLU11I BsrBI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstUI BstZ17I
ClaI CpoI DraII EagI Eam1105I
EclXI Eco47III Eco52I EcoO109I EcoO65I
EcoRV EcoT22I FseI HaeII Hin1I
HpaI KasI KpnI KspI MluI
MroI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PmaCI PmeI PmlI PpuMI
Psp1406I RsrII SacII SalI SbfI
SexAI SfiI SfoI SgrAI SnaBI
SpeI Sse8387I StuI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 4698 GTCTAC GT^[AC][GT]AC
AflIII 1080 ACGTGT A^C[GA][CT]GT
AgeI 1353 ACCGGT A^CCGGT
AseI 2698 ATTAAT AT^TAAT
AsnI 2698 ATTAAT AT^TAAT
AspI 4194 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4112 TGCGCA TGC^GCA
BalI 4409 TGGCCA TGG^CCA
BcgI 2035 GCAGGATCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2380 AGATCT A^GATCT
BlpI 4233 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4233 GCTGAGC GC^T[ACGT]AGC
BsaWI 1353 ACCGGT [AT]^CCGG[AT]
BsgI 2033 GTGCAG GTGCAG(16/14)
BsiEI 2486 CGATCG CG[GA][CT]^CG
Bsp1407I 4617 TGTACA T^GTACA
BsrFI 1353 ACCGGT [GA]^CCGG[CT]
BsrGI 4617 TGTACA T^GTACA
BstBI 1204 TTCGAA TT^CGAA
CelII 4233 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1353 ACCGGT [GA]^CCGG[CT]
DraI 1095 TTTAAA TTT^AAA
DraIII 1906 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 4409 TGGCCA [CT]^GGCC[GA]
FspI 4112 TGCGCA TGC^GCA
HgaI 4194 GACGC GACGC(5/10)
MfeI 2333 CAATTG C^AATTG
MluNI 4409 TGGCCA TGG^CCA
MscI 4409 TGGCCA TGG^CCA
MunI 2333 CAATTG C^AATTG
NcoI 282 CCATGG C^CATGG
NspV 1204 TTCGAA TT^CGAA
PaeR7I 4510 CTCGAG C^TCGAG
PflFI 4194 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1353 ACCGGT A^CCGGT
PshAI 2245 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2698 ATTAAT AT^TAAT
PvuI 2486 CGATCG CGAT^CG
SfuI 1204 TTCGAA TT^CGAA
SmaI 4017 CCCGGG CCC^GGG
SspBI 4617 TGTACA T^GTACA
SwaI 1094 ATTTAAAT ATTT^AAAT
Tth111I 4194 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 4510 CTCGAG C^TCGAG
XmaCI 4017 CCCGGG C^CCGGG
XmaI 4017 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BanI 1904 GGCACC G^G[CT][GA]CC
4354 GGTGCC
BbvCI 4387 GCTGAGG CCTCAGC(-5/-2)
4431 GCTGAGG
BciVI 276 GTATCC GTATCC(6/5)
3754 GTATCC
BsaAI 1079 TACGTG [CT]AC^GT[GA]
3310 TACGTG
BsaBI 2901 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3673 GATGTGTATC
BsaI 590 GAGACC GGTCTC(1/5)
3114 GGTCTC
BspHI 362 TCATGA T^CATGA
758 TCATGA
DrdI 1942 GACCGTGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3961 GACACAAAAGTC
DsaI 282 CCATGG C^C[GA][CT]GG
1371 CCACGG
FauI 2948 CCCGC CCCGC(4/6)
3111 GCGGG
HincII 628 GTCAAC GT[CT]^[GA]AC
2350 GTTGAC
HindII 628 GTCAAC GT[CT]^[GA]AC
2350 GTTGAC
HindIII 2182 AAGCTT A^AGCTT
4634 AAGCTT
MamI 2901 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3673 GATGTGTATC
NspI 2675 GCATGC [GA]CATG^[CT]
2863 GCATGC
RcaI 362 TCATGA T^CATGA
758 TCATGA
SacI 243 GAGCTC GAGCT^C
4723 GAGCTC
SapI 3691 GAAGAGC GCTCTTC(1/4)
670 GCTCTTC
ScaI 1488 AGTACT AGT^ACT
3434 AGTACT
SphI 2675 GCATGC GCATG^C
2863 GCATGC
XbaI 21 TCTAGA T^CTAGA
4266 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI AlwNI AocI ApoI Asp700I
AspHI AvaI AvaII BamHI BanII
BbsI BbvI BclI BcnI BfaI
BfrI BmyI BpmI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII EarI Eco81I
EcoNI EcoRI EcoRII EcoT14I FbaI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmnI