##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI AhdI ApaI AscI AseI
AsnI Asp700I AspEI AspI BbrPI
BcgI BfrI BlpI Bpu1102I BsaAI
BsaBI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BsrGI Bst1107I BstBI
BstZ17I CelII ClaI DraI DrdI
Eam1105I EcoRI EcoRV EcoT22I FseI
HincII HindII HpaI KspI MamI
MfeI MluI MroI MunI NdeI
NotI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I RcaI SacII
SalI SbfI SfiI SfuI SnaBI
SpeI Sse8387I SspBI SspI SwaI
Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AflIII 868 ACATGT A^C[GA][CT]GT
Aor51HI 44 AGCGCT AGC^GCT
AviII 1347 TGCGCA TGC^GCA
AvrII 1921 CCTAGG C^CTAGG
BbvCI 1542 GCTGAGG CCTCAGC(-5/-2)
BclI 3724 TGATCA T^GATCA
BglII 3843 AGATCT A^GATCT
BlnI 1921 CCTAGG C^CTAGG
BspLU11I 868 ACATGT A^CATGT
BsrDI 2845 GCAATG GCAATG(2/0)
BssSI 1194 CTCGTG CACGAG(-5/-1)
BstEII 1877 GGTGACC G^GT[ACGT]ACC
BstPI 1877 GGTGACC G^GT[ACGT]ACC
CpoI 392 CGGACCG CG^G[AT]CCG
Eco47III 44 AGCGCT AGC^GCT
EcoO65I 1877 GGTGACC G^GT[ACGT]ACC
FbaI 3724 TGATCA T^GATCA
FspI 1347 TGCGCA TGC^GCA
HindIII 2375 AAGCTT A^AGCTT
NheI 4107 GCTAGC G^CTAGC
NruI 449 TCGCGA TCG^CGA
PvuI 374 CGATCG CGAT^CG
RsrII 392 CGGACCG CG^G[AT]CCG
ScaI 1582 AGTACT AGT^ACT
SexAI 3217 ACCTGGT A^CC[AT]GGT
SgrAI 60 CACCGGCG C[GA]^CCGG[CT]G
SmaI 3169 CCCGGG CCC^GGG
SphI 2445 GCATGC GCATG^C
XmaCI 3169 CCCGGG C^CCGGG
XmaI 3169 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
Acc65I 3221 GGTACC G^GTACC
3836 GGTACC
Asp718I 3221 GGTACC G^GTACC
3836 GGTACC
BciVI 2692 GGATAC GTATCC(6/5)
4296 GTATCC
BsmBI 66 CGTCTC CGTCTC(1/5)
888 GAGACG
BsmI 1163 GCATTC GAATGC(1/-1)
1335 GCATTC
BstAPI 1350 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3498 GCAGAACCTGC
BstXI 1256 CCAGGGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3795 CCAAGTTATTGG
EarI 315 CTCTTC CTCTTC(1/4)
1059 CTCTTC
KpnI 3221 GGTACC GGTAC^C
3836 GGTACC
Ksp632I 315 CTCTTC CTCTTC(1/4)
1059 CTCTTC
NaeI 614 GCCGGC GCC^GGC
2357 GCCGGC
NgoMIV 614 GCCGGC G^CCGGC
2357 GCCGGC
SapI 314 GCTCTTC GCTCTTC(1/4)
1058 GCTCTTC
XbaI 2803 TCTAGA T^CTAGA
3230 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaLI ApoI AspHI AvaI AvaII
BalI BamHI BanI BanII BbeI
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssHII BssKI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EagI EclXI
Eco52I Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII