################################## # NO CUT ENZYMES ( 78 enzymes) # ################################## AatII AccI AccIII AclI AflII AgeI AhdI ApaI AscI AseI AsnI Asp700I AspEI AspI BbrPI BcgI BfrI BlpI Bpu1102I BsaAI BsaBI BseAI BsiWI Bsp1407I BspDI BspEI BspHI BsrGI Bst1107I BstBI BstZ17I CelII ClaI DraI DrdI Eam1105I EcoRI EcoRV EcoT22I FseI HincII HindII HpaI KspI MamI MfeI MluI MroI MunI NdeI NotI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I RcaI SacII SalI SbfI SfiI SfuI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I XhoI XmnI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AflIII 868 ACATGT A^C[GA][CT]GT Aor51HI 44 AGCGCT AGC^GCT AviII 1347 TGCGCA TGC^GCA AvrII 1921 CCTAGG C^CTAGG BbvCI 1542 GCTGAGG CCTCAGC(-5/-2) BclI 3724 TGATCA T^GATCA BglII 3843 AGATCT A^GATCT BlnI 1921 CCTAGG C^CTAGG BspLU11I 868 ACATGT A^CATGT BsrDI 2845 GCAATG GCAATG(2/0) BssSI 1194 CTCGTG CACGAG(-5/-1) BstEII 1877 GGTGACC G^GT[ACGT]ACC BstPI 1877 GGTGACC G^GT[ACGT]ACC CpoI 392 CGGACCG CG^G[AT]CCG Eco47III 44 AGCGCT AGC^GCT EcoO65I 1877 GGTGACC G^GT[ACGT]ACC FbaI 3724 TGATCA T^GATCA FspI 1347 TGCGCA TGC^GCA HindIII 2375 AAGCTT A^AGCTT NheI 4107 GCTAGC G^CTAGC NruI 449 TCGCGA TCG^CGA PvuI 374 CGATCG CGAT^CG RsrII 392 CGGACCG CG^G[AT]CCG ScaI 1582 AGTACT AGT^ACT SexAI 3217 ACCTGGT A^CC[AT]GGT SgrAI 60 CACCGGCG C[GA]^CCGG[CT]G SmaI 3169 CCCGGG CCC^GGG SphI 2445 GCATGC GCATG^C XmaCI 3169 CCCGGG C^CCGGG XmaI 3169 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### Acc65I 3221 GGTACC G^GTACC 3836 GGTACC Asp718I 3221 GGTACC G^GTACC 3836 GGTACC BciVI 2692 GGATAC GTATCC(6/5) 4296 GTATCC BsmBI 66 CGTCTC CGTCTC(1/5) 888 GAGACG BsmI 1163 GCATTC GAATGC(1/-1) 1335 GCATTC BstAPI 1350 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3498 GCAGAACCTGC BstXI 1256 CCAGGGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3795 CCAAGTTATTGG EarI 315 CTCTTC CTCTTC(1/4) 1059 CTCTTC KpnI 3221 GGTACC GGTAC^C 3836 GGTACC Ksp632I 315 CTCTTC CTCTTC(1/4) 1059 CTCTTC NaeI 614 GCCGGC GCC^GGC 2357 GCCGGC NgoMIV 614 GCCGGC G^CCGGC 2357 GCCGGC SapI 314 GCTCTTC GCTCTTC(1/4) 1058 GCTCTTC XbaI 2803 TCTAGA T^CTAGA 3230 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 136 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI Alw44I AlwI AlwNI AocI ApaLI ApoI AspHI AvaI AvaII BalI BamHI BanI BanII BbeI BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssHII BssKI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EagI EclXI Eco52I Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII