################################## # NO CUT ENZYMES ( 43 enzymes) # ################################## AhdI AscI AseI AsnI AspEI AviII BclI BglII BsaBI BsiWI BsmBI BstBI BstEII BstPI CpoI DrdI Eam1105I EcoO65I EcoRV FbaI FseI FspI MamI MluI NheI NotI NspV PacI PaeR7I PmeI PshAI PshBI PvuI RsrII SalI SbfI ScaI SfuI SnaBI Sse8387I SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AatII 2212 GACGTC GACGT^C Acc65I 877 GGTACC G^GTACC AccIII 512 TCCGGA T^CCGGA AclI 3852 AACGTT AA^CGTT AgeI 591 ACCGGT A^CCGGT Alw44I 3804 GTGCAC G^TGCAC Aor51HI 240 AGCGCT AGC^GCT ApaLI 3804 GTGCAC G^TGCAC Asp718I 877 GGTACC G^GTACC AvrII 575 CCTAGG C^CTAGG BbeI 744 GGCGCC GGCGC^C BbrPI 1764 CACGTG CAC^GTG BciVI 1596 GTATCC GTATCC(6/5) BlnI 575 CCTAGG C^CTAGG BsaI 601 GAGACC GGTCTC(1/5) BseAI 512 TCCGGA T^CCGGA BspDI 1549 ATCGAT AT^CGAT BspEI 512 TCCGGA T^CCGGA BspHI 4701 TCATGA T^CATGA BssHII 1174 GCGCGC G^CGCGC Bst1107I 4111 GTATAC GTA^TAC BstXI 3195 CCACAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 4111 GTATAC GTA^TAC ClaI 1549 ATCGAT AT^CGAT DraI 2955 TTTAAA TTT^AAA EagI 727 CGGCCG C^GGCCG EclXI 727 CGGCCG C^GGCCG Eco47III 240 AGCGCT AGC^GCT Eco52I 727 CGGCCG C^GGCCG HpaI 4117 GTTAAC GTT^AAC KasI 744 GGCGCC G^GCGCC KpnI 877 GGTACC GGTAC^C MfeI 3132 CAATTG C^AATTG MroI 512 TCCGGA T^CCGGA MunI 3132 CAATTG C^AATTG NarI 744 GGCGCC GG^CGCC NruI 483 TCGCGA TCG^CGA PinAI 591 ACCGGT A^CCGGT PmaCI 1764 CACGTG CAC^GTG PmlI 1764 CACGTG CAC^GTG Psp1406I 3852 AACGTT AA^CGTT RcaI 4701 TCATGA T^CATGA SacI 1891 GAGCTC GAGCT^C SapI 721 GCTCTTC GCTCTTC(1/4) SexAI 2390 ACCTGGT A^CC[AT]GGT SfiI 728 GGCCGCCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 744 GGCGCC GGC^GCC SgrAI 530 CGCCGGCG C[GA]^CCGG[CT]G SpeI 3114 ACTAGT A^CTAGT TaqI 1550 TCGA T^CGA TthHB8I 1550 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 43 enzymes) # ################################### AcyI 744 GGCGCC G[GA]^CG[CT]C 2212 GACGTC AflII 3245 CTTAAG C^TTAAG 4217 CTTAAG AocI 162 CCTGAGG CC^T[ACGT]AGG 2272 CCTCAGG Asp700I 1388 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3176 GAAACTCTTC AspI 2466 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 3902 GACAAGGTC BalI 928 TGGCCA TGG^CCA 1948 TGGCCA BbvCI 3214 CCTCAGC CCTCAGC(-5/-2) 2913 GCTGAGG BfrI 3245 CTTAAG C^TTAAG 4217 CTTAAG BlpI 617 GCTGAGC GC^T[ACGT]AGC 1050 GCTAAGC Bpu1102I 617 GCTGAGC GC^T[ACGT]AGC 1050 GCTAAGC BsaAI 1764 CACGTG [CT]AC^GT[GA] 3778 TACGTG BsaHI 744 GGCGCC G[GA]^CG[CT]C 2212 GACGTC BsmI 1677 GAATGC GAATGC(1/-1) 1628 GCATTC Bsp1407I 625 TGTACA T^GTACA 3569 TGTACA BsrBI 471 CCGCTC CCGCTC(-3/-3) 304 GAGCGG BsrDI 1984 CATTGC GCAATG(2/0) 986 GCAATG BsrGI 625 TGTACA T^GTACA 3569 TGTACA BstAPI 455 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1225 GCATGTACTGC Bsu36I 162 CCTGAGG CC^T[ACGT]AGG 2272 CCTCAGG CelII 617 GCTGAGC GC^T[ACGT]AGC 1050 GCTAAGC DraIII 1293 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG 1329 CACAAGGTG Eco81I 162 CCTGAGG CC^T[ACGT]AGG 2272 CCTCAGG EcoNI 105 CCTTCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1821 CCTTTCTGAGG EcoRI 2154 GAATTC G^AATTC 4697 GAATTC Hin1I 744 GGCGCC G[GA]^CG[CT]C 2212 GACGTC HincII 4117 GTTAAC GT[CT]^[GA]AC 4843 GTCAAC HindII 4117 GTTAAC GT[CT]^[GA]AC 4843 GTCAAC MluNI 928 TGGCCA TGG^CCA 1948 TGGCCA MscI 928 TGGCCA TGG^CCA 1948 TGGCCA NcoI 700 CCATGG C^CATGG 3396 CCATGG NdeI 2780 CATATG CA^TATG 3809 CATATG PflFI 2466 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 3902 GACAAGGTC PflMI 1579 CCATACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4434 CCAGCTCCTGG SmaI 716 CCCGGG CCC^GGG 1036 CCCGGG SspBI 625 TGTACA T^GTACA 3569 TGTACA SspI 2801 AATATT AAT^ATT 4130 AATATT StuI 572 AGGCCT AGG^CCT 2520 AGGCCT Tth111I 2466 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 3902 GACAAGGTC Van91I 1579 CCATACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4434 CCAGCTCCTGG XcmI 881 CCAGCAGGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4618 CCAAGACTAATTTGG XmaCI 716 CCCGGG C^CCGGG 1036 CCCGGG XmaI 716 CCCGGG C^CCGGG 1036 CCCGGG XmnI 1388 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3176 GAAACTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI ApaI ApoI AspHI AvaI AvaII BamHI BanI BanII BbsI BbvI BcgI BcnI BfaI BglI BmyI BpmI BpuAI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspLU11I BspMI BsrFI BsrI BssKI BssSI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NsiI NspI PleI PpuMI PstI PvuII RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII