##################################
# NO CUT ENZYMES ( 43 enzymes) #
##################################
AhdI AscI AseI AsnI AspEI
AviII BclI BglII BsaBI BsiWI
BsmBI BstBI BstEII BstPI CpoI
DrdI Eam1105I EcoO65I EcoRV FbaI
FseI FspI MamI MluI NheI
NotI NspV PacI PaeR7I PmeI
PshAI PshBI PvuI RsrII SalI
SbfI ScaI SfuI SnaBI Sse8387I
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 2212 GACGTC GACGT^C
Acc65I 877 GGTACC G^GTACC
AccIII 512 TCCGGA T^CCGGA
AclI 3852 AACGTT AA^CGTT
AgeI 591 ACCGGT A^CCGGT
Alw44I 3804 GTGCAC G^TGCAC
Aor51HI 240 AGCGCT AGC^GCT
ApaLI 3804 GTGCAC G^TGCAC
Asp718I 877 GGTACC G^GTACC
AvrII 575 CCTAGG C^CTAGG
BbeI 744 GGCGCC GGCGC^C
BbrPI 1764 CACGTG CAC^GTG
BciVI 1596 GTATCC GTATCC(6/5)
BlnI 575 CCTAGG C^CTAGG
BsaI 601 GAGACC GGTCTC(1/5)
BseAI 512 TCCGGA T^CCGGA
BspDI 1549 ATCGAT AT^CGAT
BspEI 512 TCCGGA T^CCGGA
BspHI 4701 TCATGA T^CATGA
BssHII 1174 GCGCGC G^CGCGC
Bst1107I 4111 GTATAC GTA^TAC
BstXI 3195 CCACAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 4111 GTATAC GTA^TAC
ClaI 1549 ATCGAT AT^CGAT
DraI 2955 TTTAAA TTT^AAA
EagI 727 CGGCCG C^GGCCG
EclXI 727 CGGCCG C^GGCCG
Eco47III 240 AGCGCT AGC^GCT
Eco52I 727 CGGCCG C^GGCCG
HpaI 4117 GTTAAC GTT^AAC
KasI 744 GGCGCC G^GCGCC
KpnI 877 GGTACC GGTAC^C
MfeI 3132 CAATTG C^AATTG
MroI 512 TCCGGA T^CCGGA
MunI 3132 CAATTG C^AATTG
NarI 744 GGCGCC GG^CGCC
NruI 483 TCGCGA TCG^CGA
PinAI 591 ACCGGT A^CCGGT
PmaCI 1764 CACGTG CAC^GTG
PmlI 1764 CACGTG CAC^GTG
Psp1406I 3852 AACGTT AA^CGTT
RcaI 4701 TCATGA T^CATGA
SacI 1891 GAGCTC GAGCT^C
SapI 721 GCTCTTC GCTCTTC(1/4)
SexAI 2390 ACCTGGT A^CC[AT]GGT
SfiI 728 GGCCGCCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 744 GGCGCC GGC^GCC
SgrAI 530 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 3114 ACTAGT A^CTAGT
TaqI 1550 TCGA T^CGA
TthHB8I 1550 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 43 enzymes) #
###################################
AcyI 744 GGCGCC G[GA]^CG[CT]C
2212 GACGTC
AflII 3245 CTTAAG C^TTAAG
4217 CTTAAG
AocI 162 CCTGAGG CC^T[ACGT]AGG
2272 CCTCAGG
Asp700I 1388 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3176 GAAACTCTTC
AspI 2466 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3902 GACAAGGTC
BalI 928 TGGCCA TGG^CCA
1948 TGGCCA
BbvCI 3214 CCTCAGC CCTCAGC(-5/-2)
2913 GCTGAGG
BfrI 3245 CTTAAG C^TTAAG
4217 CTTAAG
BlpI 617 GCTGAGC GC^T[ACGT]AGC
1050 GCTAAGC
Bpu1102I 617 GCTGAGC GC^T[ACGT]AGC
1050 GCTAAGC
BsaAI 1764 CACGTG [CT]AC^GT[GA]
3778 TACGTG
BsaHI 744 GGCGCC G[GA]^CG[CT]C
2212 GACGTC
BsmI 1677 GAATGC GAATGC(1/-1)
1628 GCATTC
Bsp1407I 625 TGTACA T^GTACA
3569 TGTACA
BsrBI 471 CCGCTC CCGCTC(-3/-3)
304 GAGCGG
BsrDI 1984 CATTGC GCAATG(2/0)
986 GCAATG
BsrGI 625 TGTACA T^GTACA
3569 TGTACA
BstAPI 455 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1225 GCATGTACTGC
Bsu36I 162 CCTGAGG CC^T[ACGT]AGG
2272 CCTCAGG
CelII 617 GCTGAGC GC^T[ACGT]AGC
1050 GCTAAGC
DraIII 1293 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
1329 CACAAGGTG
Eco81I 162 CCTGAGG CC^T[ACGT]AGG
2272 CCTCAGG
EcoNI 105 CCTTCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1821 CCTTTCTGAGG
EcoRI 2154 GAATTC G^AATTC
4697 GAATTC
Hin1I 744 GGCGCC G[GA]^CG[CT]C
2212 GACGTC
HincII 4117 GTTAAC GT[CT]^[GA]AC
4843 GTCAAC
HindII 4117 GTTAAC GT[CT]^[GA]AC
4843 GTCAAC
MluNI 928 TGGCCA TGG^CCA
1948 TGGCCA
MscI 928 TGGCCA TGG^CCA
1948 TGGCCA
NcoI 700 CCATGG C^CATGG
3396 CCATGG
NdeI 2780 CATATG CA^TATG
3809 CATATG
PflFI 2466 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3902 GACAAGGTC
PflMI 1579 CCATACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4434 CCAGCTCCTGG
SmaI 716 CCCGGG CCC^GGG
1036 CCCGGG
SspBI 625 TGTACA T^GTACA
3569 TGTACA
SspI 2801 AATATT AAT^ATT
4130 AATATT
StuI 572 AGGCCT AGG^CCT
2520 AGGCCT
Tth111I 2466 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3902 GACAAGGTC
Van91I 1579 CCATACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4434 CCAGCTCCTGG
XcmI 881 CCAGCAGGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4618 CCAAGACTAATTTGG
XmaCI 716 CCCGGG C^CCGGG
1036 CCCGGG
XmaI 716 CCCGGG C^CCGGG
1036 CCCGGG
XmnI 1388 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3176 GAAACTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApaI
ApoI AspHI AvaI AvaII BamHI
BanI BanII BbsI BbvI BcgI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspLU11I BspMI
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NsiI NspI
PleI PpuMI PstI PvuII RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII