##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Alw44I ApaLI AscI Asp718I AviII
BbrPI BcgI BciVI BglII BlpI
Bpu1102I BsaWI BseAI BsiEI BsiWI
BspEI BspLU11I BssHII Bst1107I BstZ17I
CelII CpoI EagI EclXI Eco52I
EcoRI EcoRV FseI FspI KpnI
KspI MroI NotI NruI PacI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI ScaI SfiI SgrAI SmaI
SpeI SphI Sse8387I SwaI XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 66 enzymes) #
###################################
AccII 2925 CGCG CG^CG
AflII 3137 CTTAAG C^TTAAG
AflIII 2924 ACGCGT A^C[GA][CT]GT
AhdI 851 GACAAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 4214 CCTGAGG CC^T[ACGT]AGG
Aor51HI 418 AGCGCT AGC^GCT
ApaI 3624 GGGCCC GGGCC^C
AspEI 851 GACAAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3659 CCTAGG C^CTAGG
BalI 1346 TGGCCA TGG^CCA
BbeI 3862 GGCGCC GGCGC^C
BbvCI 376 GCTGAGG CCTCAGC(-5/-2)
BclI 2210 TGATCA T^GATCA
BfrI 3137 CTTAAG C^TTAAG
BglI 3417 GCCAATGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3659 CCTAGG C^CTAGG
BsaAI 3797 TACGTA [CT]AC^GT[GA]
BsaBI 2998 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 2174 GAGACG CGTCTC(1/5)
BspDI 1032 ATCGAT AT^CGAT
BsrFI 3545 GCCGGC [GA]^CCGG[CT]
BssSI 3888 CACGAG CACGAG(-5/-1)
BstAPI 4229 GCAGGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3079 TTCGAA TT^CGAA
BstEII 4219 GGTCACC G^GT[ACGT]ACC
BstPI 4219 GGTCACC G^GT[ACGT]ACC
BstUI 2925 CGCG CG^CG
Bsu36I 4214 CCTGAGG CC^T[ACGT]AGG
Cfr10I 3545 GCCGGC [GA]^CCGG[CT]
ClaI 1032 ATCGAT AT^CGAT
DraIII 2301 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2365 GACACGGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1346 TGGCCA [CT]^GGCC[GA]
Eam1105I 851 GACAAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 418 AGCGCT AGC^GCT
Eco81I 4214 CCTGAGG CC^T[ACGT]AGG
EcoO65I 4219 GGTCACC G^GT[ACGT]ACC
EcoT22I 1309 ATGCAT ATGCA^T
FbaI 2210 TGATCA T^GATCA
HpaI 4708 GTTAAC GTT^AAC
KasI 3862 GGCGCC G^GCGCC
MamI 2998 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2490 CAATTG C^AATTG
MluI 2924 ACGCGT A^CGCGT
MluNI 1346 TGGCCA TGG^CCA
MscI 1346 TGGCCA TGG^CCA
MunI 2490 CAATTG C^AATTG
MvnI 2925 CGCG CG^CG
NaeI 3545 GCCGGC GCC^GGC
NarI 3862 GGCGCC GG^CGCC
NdeI 2267 CATATG CA^TATG
NgoMIV 3545 GCCGGC G^CCGGC
NheI 2725 GCTAGC G^CTAGC
NsiI 1309 ATGCAT ATGCA^T
NspI 3549 GCATGT [GA]CATG^[CT]
NspV 3079 TTCGAA TT^CGAA
PflMI 1639 CCAGCTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 82 GACCTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 4236 CTGCAG CTGCA^G
PvuII 3247 CAGCTG CAG^CTG
SapI 3477 GCTCTTC GCTCTTC(1/4)
SfoI 3862 GGCGCC GGC^GCC
SfuI 3079 TTCGAA TT^CGAA
SnaBI 3797 TACGTA TAC^GTA
SspI 1321 AATATT AAT^ATT
Van91I 1639 CCAGCTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 1975 GTAGAC GT^[AC][GT]AC
3998 GTCTAC
AcyI 3862 GGCGCC G[GA]^CG[CT]C
4064 GACGCC
AseI 13 ATTAAT AT^TAAT
1095 ATTAAT
AsnI 13 ATTAAT AT^TAAT
1095 ATTAAT
AspI 840 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2980 GACACCGTC
AvaI 73 CTCGAG C^[CT]CG[GA]G
2946 CTCGAG
BamHI 3946 GGATCC G^GATCC
4513 GGATCC
BsaHI 3862 GGCGCC G[GA]^CG[CT]C
4064 GACGCC
BseRI 379 GAGGAG GAGGAG(10/8)
2077 GAGGAG
BsoBI 73 CTCGAG C^[CT]CG[GA]G
2946 CTCGAG
Bsp1407I 3531 TGTACA T^GTACA
4048 TGTACA
BspHI 977 TCATGA T^CATGA
3097 TCATGA
BspMI 540 ACCTGC ACCTGC(4/8)
2584 ACCTGC
BsrBI 393 CCGCTC CCGCTC(-3/-3)
2426 CCGCTC
BsrGI 3531 TGTACA T^GTACA
4048 TGTACA
DraI 624 TTTAAA TTT^AAA
3233 TTTAAA
FauI 3682 CCCGC CCCGC(4/6)
2892 GCGGG
HgaI 4064 GACGC GACGC(5/10)
2926 GCGTC
Hin1I 3862 GGCGCC G[GA]^CG[CT]C
4064 GACGCC
HincII 3637 GTTGAC GT[CT]^[GA]AC
4708 GTTAAC
HindII 3637 GTTGAC GT[CT]^[GA]AC
4708 GTTAAC
NcoI 1500 CCATGG C^CATGG
2712 CCATGG
PaeR7I 73 CTCGAG C^TCGAG
2946 CTCGAG
PflFI 840 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2980 GACACCGTC
PshBI 13 ATTAAT AT^TAAT
1095 ATTAAT
RcaI 977 TCATGA T^CATGA
3097 TCATGA
SexAI 527 ACCTGGT A^CC[AT]GGT
841 ACCAGGT
SfcI 34 CTACAG C^T[GA][CT]AG
4236 CTGCAG
SspBI 3531 TGTACA T^GTACA
4048 TGTACA
Tth111I 840 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2980 GACACCGTC
XhoI 73 CTCGAG C^TCGAG
2946 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EarI EcoNI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmnI