##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AccII AccIII AclI AgeI AscI
AseI AsnI AviII BbeI BcgI
BclI BglII BseAI BsmBI BspDI
BspEI BsrBI BssHII BstBI BstEII
BstPI BstUI ClaI CpoI DraI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI HindIII HpaI
KasI KspI MfeI MluI MroI
MunI MvnI NarI NotI NruI
NsiI NspV PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SwaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 4001 GACGTC GACGT^C
AcsI 3509 AAATTC [GA]^AATT[CT]
AcyI 4001 GACGTC G[GA]^CG[CT]C
AflII 570 CTTAAG C^TTAAG
Alw44I 3070 GTGCAC G^TGCAC
Aor51HI 1726 AGCGCT AGC^GCT
ApaLI 3070 GTGCAC G^TGCAC
ApoI 3509 AAATTC [GA]^AATT[CT]
AspI 3198 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1521 CTCGAG C^[CT]CG[GA]G
BbrPI 146 CACGTG CAC^GTG
BciVI 3478 GGATAC GTATCC(6/5)
BfrI 570 CTTAAG C^TTAAG
BsaBI 2158 GATCCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 4001 GACGTC G[GA]^CG[CT]C
BsaWI 512 TCCGGT [AT]^CCGG[AT]
BsiWI 2036 CGTACG C^GTACG
BsoBI 1521 CTCGAG C^[CT]CG[GA]G
Bsp1407I 182 TGTACA T^GTACA
BspHI 156 TCATGA T^CATGA
BspLU11I 2535 ACATGT A^CATGT
BsrGI 182 TGTACA T^GTACA
Bst1107I 806 GTATAC GTA^TAC
BstAPI 644 GCAGGAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 806 GTATAC GTA^TAC
DrdI 3394 GACCCAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 22 CGGCCG C^GGCCG
EclXI 22 CGGCCG C^GGCCG
Eco47III 1726 AGCGCT AGC^GCT
Eco52I 22 CGGCCG C^GGCCG
FauI 1429 GCGGG CCCGC(4/6)
HaeII 1726 AGCGCT [GA]GCGC^[CT]
Hin1I 4001 GACGTC G[GA]^CG[CT]C
MamI 2158 GATCCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 832 GCCGGC GCC^GGC
NdeI 654 CATATG CA^TATG
NgoMIV 832 GCCGGC G^CCGGC
NheI 3942 GCTAGC G^CTAGC
PaeR7I 1521 CTCGAG C^TCGAG
PflFI 3198 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 146 CACGTG CAC^GTG
PmlI 146 CACGTG CAC^GTG
RcaI 156 TCATGA T^CATGA
SapI 2379 GCTCTTC GCTCTTC(1/4)
ScaI 1456 AGTACT AGT^ACT
SphI 1069 GCATGC GCATG^C
SspBI 182 TGTACA T^GTACA
SspI 1982 AATATT AAT^ATT
Tth111I 3198 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1130 TCTAGA T^CTAGA
XhoI 1521 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 595 GGTACC G^GTACC
2834 GGTACC
AccI 806 GTATAC GT^[AC][GT]AC
2632 GTCTAC
AhdI 2582 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3445 GACAGAAGGTC
AocI 1958 CCTTAGG CC^T[ACGT]AGG
2132 CCTGAGG
ApaI 867 GGGCCC GGGCC^C
2913 GGGCCC
Asp700I 2376 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3508 GAAATTCTTC
Asp718I 595 GGTACC G^GTACC
2834 GGTACC
AspEI 2582 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3445 GACAGAAGGTC
AvrII 138 CCTAGG C^CTAGG
2275 CCTAGG
BamHI 1785 GGATCC G^GATCC
3620 GGATCC
BbsI 388 GTCTTC GAAGAC(2/6)
3415 GTCTTC
BlnI 138 CCTAGG C^CTAGG
2275 CCTAGG
BpuAI 388 GTCTTC GAAGAC(2/6)
3415 GTCTTC
BsaAI 146 CACGTG [CT]AC^GT[GA]
609 CACGTA
BsiEI 22 CGGCCG CG[GA][CT]^CG
3095 CGACCG
BsmI 999 GAATGC GAATGC(1/-1)
2998 GAATGC
BsrDI 402 CATTGC GCAATG(2/0)
1087 CATTGC
BsrFI 832 GCCGGC [GA]^CCGG[CT]
3097 ACCGGC
BssSI 2249 CACGAG CACGAG(-5/-1)
1652 CTCGTG
Bsu36I 1958 CCTTAGG CC^T[ACGT]AGG
2132 CCTGAGG
Cfr10I 832 GCCGGC [GA]^CCGG[CT]
3097 ACCGGC
Eam1105I 2582 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3445 GACAGAAGGTC
Eco81I 1958 CCTTAGG CC^T[ACGT]AGG
2132 CCTGAGG
EcoNI 878 CCTGCGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2927 CCTGGGTGAGG
HincII 2620 GTCAAC GT[CT]^[GA]AC
3926 GTTGAC
HindII 2620 GTCAAC GT[CT]^[GA]AC
3926 GTTGAC
KpnI 595 GGTACC GGTAC^C
2834 GGTACC
NspI 1069 GCATGC [GA]CATG^[CT]
2535 ACATGT
SacI 1126 GAGCTC GAGCT^C
2451 GAGCTC
SbfI 253 CCTGCAGG CCTGCA^GG
3049 CCTGCAGG
Sse8387I 253 CCTGCAGG CCTGCA^GG
3049 CCTGCAGG
StuI 3715 AGGCCT AGG^CCT
3790 AGGCCT
TaqI 1522 TCGA T^CGA
2374 TCGA
Tsp509I 3510 AATT ^AATT
4288 AATT
TthHB8I 1522 TCGA T^CGA
2374 TCGA
XcmI 884 CCAGGCCCTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2385 CCAAGAGGATGGTGG
XmnI 2376 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3508 GAAATTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI AspHI AvaII BalI BanI
BanII BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
StyI TfiI Tru9I TseI Tsp45I
TspRI Van91I XhoII