##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI BamHI
BcgI BsaBI BsaWI BseAI BsiWI
BspDI BspEI BspHI BssHII BssSI
BstBI ClaI CpoI DraIII DrdI
Eam1105I EcoRI EcoRV FseI HindIII
KpnI MamI MfeI MroI MunI
NaeI NdeI NgoMIV NotI NruI
NspV PacI PaeR7I PflMI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI Van91I
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AcsI 4351 AAATTT [GA]^AATT[CT]
AflII 2965 CTTAAG C^TTAAG
Aor51HI 3098 AGCGCT AGC^GCT
ApoI 4351 AAATTT [GA]^AATT[CT]
AviII 1923 TGCGCA TGC^GCA
AvrII 2808 CCTAGG C^CTAGG
BciVI 881 GTATCC GTATCC(6/5)
BfrI 2965 CTTAAG C^TTAAG
BglII 4292 AGATCT A^GATCT
BlnI 2808 CCTAGG C^CTAGG
BsiEI 62 CGGCCG CG[GA][CT]^CG
BsmI 4132 GCATTC GAATGC(1/-1)
Bsp1407I 1412 TGTACA T^GTACA
BsrFI 545 ACCGGC [GA]^CCGG[CT]
BsrGI 1412 TGTACA T^GTACA
Bst1107I 4329 GTATAC GTA^TAC
BstZ17I 4329 GTATAC GTA^TAC
Cfr10I 545 ACCGGC [GA]^CCGG[CT]
EagI 62 CGGCCG C^GGCCG
EclXI 62 CGGCCG C^GGCCG
Eco47III 3098 AGCGCT AGC^GCT
Eco52I 62 CGGCCG C^GGCCG
FspI 1923 TGCGCA TGC^GCA
HincII 2977 GTTAAC GT[CT]^[GA]AC
HindII 2977 GTTAAC GT[CT]^[GA]AC
HpaI 2977 GTTAAC GTT^AAC
KspI 2128 CCGCGG CCGC^GG
MluI 1729 ACGCGT A^CGCGT
NcoI 588 CCATGG C^CATGG
NheI 3691 GCTAGC G^CTAGC
SacII 2128 CCGCGG CCGC^GG
SspBI 1412 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 1057 GTAGAC GT^[AC][GT]AC
4329 GTATAC
ApaI 153 GGGCCC GGGCC^C
3860 GGGCCC
BalI 359 TGGCCA TGG^CCA
2318 TGGCCA
BbeI 265 GGCGCC GGCGC^C
3034 GGCGCC
BbrPI 3450 CACGTG CAC^GTG
4266 CACGTG
BclI 3784 TGATCA T^GATCA
3945 TGATCA
BsaAI 3450 CACGTG [CT]AC^GT[GA]
4266 CACGTG
BsgI 541 CTGCAC GTGCAG(16/14)
3661 CTGCAC
BsmBI 1726 GAGACG CGTCTC(1/5)
4036 GAGACG
BspLU11I 494 ACATGT A^CATGT
1484 ACATGT
BsrBI 1749 CCGCTC CCGCTC(-3/-3)
1690 GAGCGG
BsrDI 1209 CATTGC GCAATG(2/0)
3434 CATTGC
BstEII 2862 GGTTACC G^GT[ACGT]ACC
3499 GGTTACC
BstPI 2862 GGTTACC G^GT[ACGT]ACC
3499 GGTTACC
DraI 3804 TTTAAA TTT^AAA
3818 TTTAAA
EcoO65I 2862 GGTTACC G^GT[ACGT]ACC
3499 GGTTACC
EcoT22I 1765 ATGCAT ATGCA^T
1982 ATGCAT
FbaI 3784 TGATCA T^GATCA
3945 TGATCA
KasI 265 GGCGCC G^GCGCC
3034 GGCGCC
MluNI 359 TGGCCA TGG^CCA
2318 TGGCCA
MscI 359 TGGCCA TGG^CCA
2318 TGGCCA
NarI 265 GGCGCC GG^CGCC
3034 GGCGCC
NsiI 1765 ATGCAT ATGCA^T
1982 ATGCAT
PmaCI 3450 CACGTG CAC^GTG
4266 CACGTG
PmlI 3450 CACGTG CAC^GTG
4266 CACGTG
PstI 1973 CTGCAG CTGCA^G
3029 CTGCAG
SapI 2563 GCTCTTC GCTCTTC(1/4)
4230 GCTCTTC
SfoI 265 GGCGCC GGC^GCC
3034 GGCGCC
SmlI 2279 CTTGAG C^T[CT][GA]AG
2965 CTTAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI AspHI
AspI AvaI AvaII BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflFI PleI PpuMI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SphI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XcmI XhoII XmaCI XmaI