##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AclI AcsI AflII AgeI AhdI
Alw44I ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AviII BamHI
BbrPI BfrI BsaBI BsiEI BsiWI
BsmBI BspDI BspLU11I BsrBI BsrDI
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRI EcoRV FseI FspI HpaI
MamI MfeI MluI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SalI ScaI SfiI SfuI
SgrAI SnaBI SpeI SspI SwaI
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 1750 GACGTC GACGT^C
Acc65I 1504 GGTACC G^GTACC
AccI 2497 GTAGAC GT^[AC][GT]AC
AccIII 2225 TCCGGA T^CCGGA
AflIII 3050 ACGTGT A^C[GA][CT]GT
Asp718I 1504 GGTACC G^GTACC
AvrII 282 CCTAGG C^CTAGG
BbeI 15 GGCGCC GGCGC^C
BcgI 770 CGACCAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 167 TGATCA T^GATCA
BglII 1529 AGATCT A^GATCT
BlnI 282 CCTAGG C^CTAGG
BsaWI 2225 TCCGGA [AT]^CCGG[AT]
BseAI 2225 TCCGGA T^CCGGA
BsmI 1903 GAATGC GAATGC(1/-1)
Bsp1407I 364 TGTACA T^GTACA
BspEI 2225 TCCGGA T^CCGGA
BsrFI 2339 ACCGGC [GA]^CCGG[CT]
BsrGI 364 TGTACA T^GTACA
BssHII 49 GCGCGC G^CGCGC
Cfr10I 2339 ACCGGC [GA]^CCGG[CT]
DraIII 964 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT22I 2026 ATGCAT ATGCA^T
FbaI 167 TGATCA T^GATCA
HindIII 2230 AAGCTT A^AGCTT
KasI 15 GGCGCC G^GCGCC
KpnI 1504 GGTACC GGTAC^C
KspI 3472 CCGCGG CCGC^GG
MroI 2225 TCCGGA T^CCGGA
NarI 15 GGCGCC GG^CGCC
NsiI 2026 ATGCAT ATGCA^T
PshAI 3673 GACCCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 3472 CCGCGG CCGC^GG
SbfI 2875 CCTGCAGG CCTGCA^GG
SfoI 15 GGCGCC GGC^GCC
SphI 2837 GCATGC GCATG^C
Sse8387I 2875 CCTGCAGG CCTGCA^GG
SspBI 364 TGTACA T^GTACA
Tsp509I 2640 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcyI 15 GGCGCC G[GA]^CG[CT]C
1750 GACGTC
AspI 355 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1549 GACACTGTC
BsaHI 15 GGCGCC G[GA]^CG[CT]C
1750 GACGTC
BspHI 129 TCATGA T^CATGA
1406 TCATGA
BstYI 1529 AGATCT [GA]^GATC[CT]
3289 GGATCT
DraI 3778 TTTAAA TTT^AAA
3911 TTTAAA
HgaI 59 GACGC GACGC(5/10)
136 GACGC
Hin1I 15 GGCGCC G[GA]^CG[CT]C
1750 GACGTC
HincII 1126 GTCAAC GT[CT]^[GA]AC
3784 GTTGAC
HindII 1126 GTCAAC GT[CT]^[GA]AC
3784 GTTGAC
MflI 1529 AGATCT [GA]^GATC[CT]
3289 GGATCT
MslI 2277 CATCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3842 CATCTTAGTG
NspI 2837 GCATGC [GA]CATG^[CT]
3370 GCATGT
PflFI 355 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1549 GACACTGTC
RcaI 129 TCATGA T^CATGA
1406 TCATGA
SapI 643 GAAGAGC GCTCTTC(1/4)
2915 GAAGAGC
SexAI 1637 ACCTGGT A^CC[AT]GGT
2093 ACCAGGT
Tth111I 355 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1549 GACACTGTC
XhoII 1529 AGATCT [GA]^GATC[CT]
3289 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI Aor51HI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BciVI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstUI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco47III Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I TspRI TthHB8I Van91I XcmI
XmaCI XmaI