################################## # NO CUT ENZYMES ( 78 enzymes) # ################################## AclI AcsI AflII AgeI AhdI Alw44I ApaLI ApoI AscI AseI AsnI Asp700I AspEI AviII BamHI BbrPI BfrI BsaBI BsiEI BsiWI BsmBI BspDI BspLU11I BsrBI BsrDI BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I ClaI CpoI DrdI EagI Eam1105I EclXI Eco52I EcoO65I EcoRI EcoRV FseI FspI HpaI MamI MfeI MluI MunI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SalI ScaI SfiI SfuI SgrAI SnaBI SpeI SspI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AatII 1750 GACGTC GACGT^C Acc65I 1504 GGTACC G^GTACC AccI 2497 GTAGAC GT^[AC][GT]AC AccIII 2225 TCCGGA T^CCGGA AflIII 3050 ACGTGT A^C[GA][CT]GT Asp718I 1504 GGTACC G^GTACC AvrII 282 CCTAGG C^CTAGG BbeI 15 GGCGCC GGCGC^C BcgI 770 CGACCAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 167 TGATCA T^GATCA BglII 1529 AGATCT A^GATCT BlnI 282 CCTAGG C^CTAGG BsaWI 2225 TCCGGA [AT]^CCGG[AT] BseAI 2225 TCCGGA T^CCGGA BsmI 1903 GAATGC GAATGC(1/-1) Bsp1407I 364 TGTACA T^GTACA BspEI 2225 TCCGGA T^CCGGA BsrFI 2339 ACCGGC [GA]^CCGG[CT] BsrGI 364 TGTACA T^GTACA BssHII 49 GCGCGC G^CGCGC Cfr10I 2339 ACCGGC [GA]^CCGG[CT] DraIII 964 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoT22I 2026 ATGCAT ATGCA^T FbaI 167 TGATCA T^GATCA HindIII 2230 AAGCTT A^AGCTT KasI 15 GGCGCC G^GCGCC KpnI 1504 GGTACC GGTAC^C KspI 3472 CCGCGG CCGC^GG MroI 2225 TCCGGA T^CCGGA NarI 15 GGCGCC GG^CGCC NsiI 2026 ATGCAT ATGCA^T PshAI 3673 GACCCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 3472 CCGCGG CCGC^GG SbfI 2875 CCTGCAGG CCTGCA^GG SfoI 15 GGCGCC GGC^GCC SphI 2837 GCATGC GCATG^C Sse8387I 2875 CCTGCAGG CCTGCA^GG SspBI 364 TGTACA T^GTACA Tsp509I 2640 AATT ^AATT ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcyI 15 GGCGCC G[GA]^CG[CT]C 1750 GACGTC AspI 355 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1549 GACACTGTC BsaHI 15 GGCGCC G[GA]^CG[CT]C 1750 GACGTC BspHI 129 TCATGA T^CATGA 1406 TCATGA BstYI 1529 AGATCT [GA]^GATC[CT] 3289 GGATCT DraI 3778 TTTAAA TTT^AAA 3911 TTTAAA HgaI 59 GACGC GACGC(5/10) 136 GACGC Hin1I 15 GGCGCC G[GA]^CG[CT]C 1750 GACGTC HincII 1126 GTCAAC GT[CT]^[GA]AC 3784 GTTGAC HindII 1126 GTCAAC GT[CT]^[GA]AC 3784 GTTGAC MflI 1529 AGATCT [GA]^GATC[CT] 3289 GGATCT MslI 2277 CATCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 3842 CATCTTAGTG NspI 2837 GCATGC [GA]CATG^[CT] 3370 GCATGT PflFI 355 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1549 GACACTGTC RcaI 129 TCATGA T^CATGA 1406 TCATGA SapI 643 GAAGAGC GCTCTTC(1/4) 2915 GAAGAGC SexAI 1637 ACCTGGT A^CC[AT]GGT 2093 ACCAGGT Tth111I 355 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1549 GACACTGTC XhoII 1529 AGATCT [GA]^GATC[CT] 3289 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 122 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AocI Aor51HI ApaI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BciVI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstUI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco47III Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I TspRI TthHB8I Van91I XcmI XmaCI XmaI