##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AflIII AgeI Aor51HI AscI AseI
AsnI AviII AvrII BbeI BbrPI
BcgI BclI BfrI BglI BlnI
BsaAI BsaWI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BspLU11I BsrBI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I ClaI CpoI
DraI DrdI EagI EclXI Eco47III
Eco52I EcoO65I EcoT22I FbaI FseI
FspI HpaI KasI KspI MluI
MroI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 651 GGTACC G^GTACC
AcyI 2385 GACGCC G[GA]^CG[CT]C
AhdI 1345 GACGTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 651 GGTACC G^GTACC
AspEI 1345 GACGTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 429 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2551 TGGCCA TGG^CCA
BcnI 4051 CCCGG CC^[GC]GG
BglII 43 AGATCT A^GATCT
BsaBI 3528 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2385 GACGCC G[GA]^CG[CT]C
Bsp1407I 4499 TGTACA T^GTACA
BspMI 4310 ACCTGC ACCTGC(4/8)
BsrGI 4499 TGTACA T^GTACA
Eam1105I 1345 GACGTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 923 GAATTC G^AATTC
FauI 4117 GCGGG CCCGC(4/6)
HgaI 2385 GACGC GACGC(5/10)
Hin1I 2385 GACGCC G[GA]^CG[CT]C
HindIII 3815 AAGCTT A^AGCTT
KpnI 651 GGTACC GGTAC^C
MamI 3528 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2118 CAATTG C^AATTG
MluNI 2551 TGGCCA TGG^CCA
MscI 2551 TGGCCA TGG^CCA
MunI 2118 CAATTG C^AATTG
NciI 4051 CCCGG CC^[GC]GG
NspI 1723 ACATGC [GA]CATG^[CT]
PaeR7I 3235 CTCGAG C^TCGAG
PflFI 429 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
SalI 1440 GTCGAC G^TCGAC
ScaI 2151 AGTACT AGT^ACT
SspBI 4499 TGTACA T^GTACA
Tth111I 429 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3235 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 1440 GTCGAC GT^[AC][GT]AC
3717 GTCTAC
Alw44I 1241 GTGCAC G^TGCAC
4066 GTGCAC
ApaI 1304 GGGCCC GGGCC^C
4363 GGGCCC
ApaLI 1241 GTGCAC G^TGCAC
4066 GTGCAC
Asp700I 923 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4428 GAAATGATTC
BamHI 3266 GGATCC G^GATCC
3517 GGATCC
BbvCI 1110 CCTCAGC CCTCAGC(-5/-2)
3422 CCTCAGC
BsaI 780 GAGACC GGTCTC(1/5)
2864 GGTCTC
BsgI 3658 CTGCAC GTGCAG(16/14)
2280 GTGCAG
BsmI 4290 GAATGC GAATGC(1/-1)
439 GCATTC
BspHI 1341 TCATGA T^CATGA
1773 TCATGA
BsrDI 960 CATTGC GCAATG(2/0)
1471 CATTGC
BsrFI 1567 GCCGGT [GA]^CCGG[CT]
3450 ACCGGC
BssSI 995 CACGAG CACGAG(-5/-1)
1587 CTCGTG
BstXI 1740 CCAGTAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1788 CCAGTACGCTGG
Cfr10I 1567 GCCGGT [GA]^CCGG[CT]
3450 ACCGGC
EarI 218 CTCTTC CTCTTC(1/4)
1823 CTCTTC
EcoRV 1337 GATATC GAT^ATC
3690 GATATC
HaeII 1798 GGCGCT [GA]GCGC^[CT]
3471 AGCGCC
HincII 1440 GTCGAC GT[CT]^[GA]AC
2599 GTCAAC
HindII 1440 GTCGAC GT[CT]^[GA]AC
2599 GTCAAC
Ksp632I 218 CTCTTC CTCTTC(1/4)
1823 CTCTTC
MaeII 1346 ACGT A^CGT
4260 ACGT
RcaI 1341 TCATGA T^CATGA
1773 TCATGA
XcmI 565 CCAGCCTGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3845 CCAAGAATCCCATGG
XmnI 923 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4428 GAAATGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BciVI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI Eco81I EcoNI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NcoI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII