##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Aor51HI
AscI Asp718I AspEI AspI AviII
BalI BbeI BbrPI BcgI BfrI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI BspDI BspEI BsrBI BssHII
BstUI CelII ClaI CpoI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV EcoT22I FseI FspI HaeII
Hin1I KasI KpnI KspI MamI
MluI MluNI MroI MscI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PaeR7I PflFI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SgrAI SnaBI Sse8387I StuI SwaI
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Alw44I 1704 GTGCAC G^TGCAC
ApaI 4011 GGGCCC GGGCC^C
ApaLI 1704 GTGCAC G^TGCAC
AseI 2463 ATTAAT AT^TAAT
AsnI 2463 ATTAAT AT^TAAT
Asp700I 3839 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 4534 CCTAGG C^CTAGG
BamHI 1649 GGATCC G^GATCC
BclI 1628 TGATCA T^GATCA
BglI 384 GCCAAGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4558 AGATCT A^GATCT
BlnI 4534 CCTAGG C^CTAGG
BsgI 475 GTGCAG GTGCAG(16/14)
BspHI 835 TCATGA T^CATGA
BssSI 3372 CTCGTG CACGAG(-5/-1)
Bst1107I 892 GTATAC GTA^TAC
BstAPI 1183 GCATGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 397 TTCGAA TT^CGAA
BstEII 4398 GGTTACC G^GT[ACGT]ACC
BstPI 4398 GGTTACC G^GT[ACGT]ACC
BstZ17I 892 GTATAC GTA^TAC
CfoI 257 GCGC GCG^C
DraIII 2539 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1968 GACACAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1887 CCATGG C^C[GA][CT]GG
EcoO65I 4398 GGTTACC G^GT[ACGT]ACC
EcoRI 1414 GAATTC G^AATTC
FauI 158 GCGGG CCCGC(4/6)
FbaI 1628 TGATCA T^GATCA
HhaI 257 GCGC GCG^C
HinP1I 257 GCGC G^CGC
HindIII 1855 AAGCTT A^AGCTT
HpaI 1513 GTTAAC GTT^AAC
MfeI 942 CAATTG C^AATTG
MunI 942 CAATTG C^AATTG
NcoI 1887 CCATGG C^CATGG
NspV 397 TTCGAA TT^CGAA
PacI 3985 TTAATTAA TTAAT^TAA
PflMI 2538 CCACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 4245 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2463 ATTAAT AT^TAAT
RcaI 835 TCATGA T^CATGA
SacI 1818 GAGCTC GAGCT^C
SfuI 397 TTCGAA TT^CGAA
SmaI 15 CCCGGG CCC^GGG
SphI 1183 GCATGC GCATG^C
SspI 2717 AATATT AAT^ATT
Van91I 2538 CCACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 4143 TCTAGA T^CTAGA
XmaCI 15 CCCGGG C^CCGGG
XmaI 15 CCCGGG C^CCGGG
XmnI 3839 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AocI 3884 CCTTAGG CC^T[ACGT]AGG
4039 CCTAAGG
AvaI 15 CCCGGG C^[CT]CG[GA]G
4373 CCCGAG
BbvCI 1653 CCTCAGC CCTCAGC(-5/-2)
3661 GCTGAGG
BciVI 807 GTATCC GTATCC(6/5)
2128 GTATCC
BcnI 15 CCCGG CC^[GC]GG
16 CCGGG
BsaI 828 GAGACC GGTCTC(1/5)
2926 GGTCTC
BsoBI 15 CCCGGG C^[CT]CG[GA]G
4373 CCCGAG
BspLU11I 103 ACATGT A^CATGT
2630 ACATGT
BsrFI 706 ACCGGC [GA]^CCGG[CT]
1039 ACCGGC
BstXI 2091 CCAGAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3311 CCACAAAATTGG
Bsu36I 3884 CCTTAGG CC^T[ACGT]AGG
4039 CCTAAGG
Cfr10I 706 ACCGGC [GA]^CCGG[CT]
1039 ACCGGC
Eco81I 3884 CCTTAGG CC^T[ACGT]AGG
4039 CCTAAGG
HgaI 360 GACGC GACGC(5/10)
416 GACGC
HincII 981 GTCAAC GT[CT]^[GA]AC
1513 GTTAAC
HindII 981 GTCAAC GT[CT]^[GA]AC
1513 GTTAAC
MslI 104 CATGTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
275 CATCTATATG
NciI 15 CCCGG CC^[GC]GG
16 CCGGG
PpuMI 1403 AGGTCCT [GA]G^G[AT]CC[CT]
3553 AGGTCCT
SpeI 1922 ACTAGT A^CTAGT
3740 ACTAGT
XcmI 2109 CCAGGCTGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2947 CCAGTGCCACTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaII BanI BanII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BspMI BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraII EarI EcoNI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII