################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI Aor51HI AscI Asp718I AspEI AspI AviII BalI BbeI BbrPI BcgI BfrI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BspDI BspEI BsrBI BssHII BstUI CelII ClaI CpoI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoRV EcoT22I FseI FspI HaeII Hin1I KasI KpnI KspI MamI MluI MluNI MroI MscI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PaeR7I PflFI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SnaBI Sse8387I StuI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Alw44I 1704 GTGCAC G^TGCAC ApaI 4011 GGGCCC GGGCC^C ApaLI 1704 GTGCAC G^TGCAC AseI 2463 ATTAAT AT^TAAT AsnI 2463 ATTAAT AT^TAAT Asp700I 3839 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 4534 CCTAGG C^CTAGG BamHI 1649 GGATCC G^GATCC BclI 1628 TGATCA T^GATCA BglI 384 GCCAAGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 4558 AGATCT A^GATCT BlnI 4534 CCTAGG C^CTAGG BsgI 475 GTGCAG GTGCAG(16/14) BspHI 835 TCATGA T^CATGA BssSI 3372 CTCGTG CACGAG(-5/-1) Bst1107I 892 GTATAC GTA^TAC BstAPI 1183 GCATGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 397 TTCGAA TT^CGAA BstEII 4398 GGTTACC G^GT[ACGT]ACC BstPI 4398 GGTTACC G^GT[ACGT]ACC BstZ17I 892 GTATAC GTA^TAC CfoI 257 GCGC GCG^C DraIII 2539 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1968 GACACAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1887 CCATGG C^C[GA][CT]GG EcoO65I 4398 GGTTACC G^GT[ACGT]ACC EcoRI 1414 GAATTC G^AATTC FauI 158 GCGGG CCCGC(4/6) FbaI 1628 TGATCA T^GATCA HhaI 257 GCGC GCG^C HinP1I 257 GCGC G^CGC HindIII 1855 AAGCTT A^AGCTT HpaI 1513 GTTAAC GTT^AAC MfeI 942 CAATTG C^AATTG MunI 942 CAATTG C^AATTG NcoI 1887 CCATGG C^CATGG NspV 397 TTCGAA TT^CGAA PacI 3985 TTAATTAA TTAAT^TAA PflMI 2538 CCACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 4245 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 2463 ATTAAT AT^TAAT RcaI 835 TCATGA T^CATGA SacI 1818 GAGCTC GAGCT^C SfuI 397 TTCGAA TT^CGAA SmaI 15 CCCGGG CCC^GGG SphI 1183 GCATGC GCATG^C SspI 2717 AATATT AAT^ATT Van91I 2538 CCACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 4143 TCTAGA T^CTAGA XmaCI 15 CCCGGG C^CCGGG XmaI 15 CCCGGG C^CCGGG XmnI 3839 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AocI 3884 CCTTAGG CC^T[ACGT]AGG 4039 CCTAAGG AvaI 15 CCCGGG C^[CT]CG[GA]G 4373 CCCGAG BbvCI 1653 CCTCAGC CCTCAGC(-5/-2) 3661 GCTGAGG BciVI 807 GTATCC GTATCC(6/5) 2128 GTATCC BcnI 15 CCCGG CC^[GC]GG 16 CCGGG BsaI 828 GAGACC GGTCTC(1/5) 2926 GGTCTC BsoBI 15 CCCGGG C^[CT]CG[GA]G 4373 CCCGAG BspLU11I 103 ACATGT A^CATGT 2630 ACATGT BsrFI 706 ACCGGC [GA]^CCGG[CT] 1039 ACCGGC BstXI 2091 CCAGAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3311 CCACAAAATTGG Bsu36I 3884 CCTTAGG CC^T[ACGT]AGG 4039 CCTAAGG Cfr10I 706 ACCGGC [GA]^CCGG[CT] 1039 ACCGGC Eco81I 3884 CCTTAGG CC^T[ACGT]AGG 4039 CCTAAGG HgaI 360 GACGC GACGC(5/10) 416 GACGC HincII 981 GTCAAC GT[CT]^[GA]AC 1513 GTTAAC HindII 981 GTCAAC GT[CT]^[GA]AC 1513 GTTAAC MslI 104 CATGTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 275 CATCTATATG NciI 15 CCCGG CC^[GC]GG 16 CCGGG PpuMI 1403 AGGTCCT [GA]G^G[AT]CC[CT] 3553 AGGTCCT SpeI 1922 ACTAGT A^CTAGT 3740 ACTAGT XcmI 2109 CCAGGCTGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2947 CCAGTGCCACTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 93 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaII BanI BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspMI BsrDI BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII EarI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII