##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
Acc65I AccII AgeI Aor51HI AscI
AseI AsnI Asp718I AviII AvrII
BalI BbrPI BcgI BclI BlnI
BsaAI BsaBI BsiEI BsmBI BspDI
BsrDI BsrFI BssHII Bst1107I BstBI
BstUI BstZ17I Cfr10I ClaI CpoI
EagI EclXI Eco47III Eco52I EcoT22I
FbaI FseI FspI KpnI KspI
MamI MluI MluNI MscI MvnI
NaeI NgoMIV NotI NruI NsiI
NspV PinAI PmaCI PmeI PmlI
PshBI PvuI RsrII SacII SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI Sse8387I SwaI XbaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 3372 GACGTC GACGT^C
AccIII 4060 TCCGGA T^CCGGA
AclI 3727 AACGTT AA^CGTT
AflII 5196 CTTAAG C^TTAAG
AflIII 2900 ACATGT A^C[GA][CT]GT
AhdI 95 GACTATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 3671 GGGCCC GGGCC^C
AspEI 95 GACTATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2062 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1333 GGCGCC GGCGC^C
BciVI 1902 GGATAC GTATCC(6/5)
BfrI 5196 CTTAAG C^TTAAG
BglI 2665 GCCACAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 4060 TCCGGA [AT]^CCGG[AT]
BseAI 4060 TCCGGA T^CCGGA
BsiWI 4481 CGTACG C^GTACG
BspEI 4060 TCCGGA T^CCGGA
BspLU11I 2900 ACATGT A^CATGT
BsrBI 4407 GAGCGG CCGCTC(-3/-3)
BstEII 3591 GGTTACC G^GT[ACGT]ACC
BstPI 3591 GGTTACC G^GT[ACGT]ACC
CfoI 1334 GCGC GCG^C
DraIII 3210 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 95 GACTATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3591 GGTTACC G^GT[ACGT]ACC
EcoRV 462 GATATC GAT^ATC
FauI 1557 CCCGC CCCGC(4/6)
HaeII 1333 GGCGCC [GA]GCGC^[CT]
HhaI 1334 GCGC GCG^C
HinP1I 1334 GCGC G^CGC
KasI 1333 GGCGCC G^GCGCC
MroI 4060 TCCGGA T^CCGGA
NarI 1333 GGCGCC GG^CGCC
PacI 5094 TTAATTAA TTAAT^TAA
PaeR7I 63 CTCGAG C^TCGAG
PflFI 2062 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 3727 AACGTT AA^CGTT
SacI 1252 GAGCTC GAGCT^C
SalI 844 GTCGAC G^TCGAC
SapI 455 GCTCTTC GCTCTTC(1/4)
SfoI 1333 GGCGCC GGC^GCC
SmaI 1390 CCCGGG CCC^GGG
SpeI 2333 ACTAGT A^CTAGT
SphI 3660 GCATGC GCATG^C
StuI 1397 AGGCCT AGG^CCT
Tth111I 2062 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 63 CTCGAG C^TCGAG
XmaCI 1390 CCCGGG C^CCGGG
XmaI 1390 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BglII 3157 AGATCT A^GATCT
5804 AGATCT
Bsp1407I 201 TGTACA T^GTACA
2204 TGTACA
BspHI 3313 TCATGA T^CATGA
4664 TCATGA
BsrGI 201 TGTACA T^GTACA
2204 TGTACA
BstXI 2479 CCACACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4433 CCAGCTTACTGG
DrdI 1051 GACCCTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2409 GACAGCACAGTC
EaeI 591 CGGCCA [CT]^GGCC[GA]
1520 CGGCCA
EcoRI 3765 GAATTC G^AATTC
5191 GAATTC
HpaI 3963 GTTAAC GTT^AAC
4238 GTTAAC
MfeI 4684 CAATTG C^AATTG
5488 CAATTG
MunI 4684 CAATTG C^AATTG
5488 CAATTG
NdeI 3028 CATATG CA^TATG
3720 CATATG
PflMI 262 CCAAGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3178 CCAAGGGCTGG
PshAI 887 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5371 GACACAGGTC
PstI 952 CTGCAG CTGCA^G
1055 CTGCAG
RcaI 3313 TCATGA T^CATGA
4664 TCATGA
SspBI 201 TGTACA T^GTACA
2204 TGTACA
Van91I 262 CCAAGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3178 CCAAGGGCTGG
XcmI 1157 CCATCAAATTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3106 CCATGGCTGTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaLI ApoI Asp700I AspHI AvaI
AvaII BamHI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstYI
Bsu36I Cac8I CelII Cfr13I DdeI
DpnII DraI DraII DsaI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
Hin1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NheI NlaIII NlaIV NspI PleI
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI