##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII AclI AgeI Aor51HI AscI
Asp700I AvrII BamHI BglII BlnI
BsaBI BsiEI BsiWI BspHI BssHII
BstAPI BstBI CpoI DraI EagI
EclXI Eco47III Eco52I EcoRI FseI
HpaI MamI MfeI MluI MunI
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshAI Psp1406I
PvuI RcaI RsrII SalI ScaI
SfiI SfuI SgrAI SnaBI SpeI
SspI StuI SwaI XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AccIII 1045 TCCGGA T^CCGGA
AhdI 5294 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 4731 ATTAAT AT^TAAT
AsnI 4731 ATTAAT AT^TAAT
AspEI 5294 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 3402 GGCGCC GGCGC^C
BseAI 1045 TCCGGA T^CCGGA
BsmBI 4880 CGTCTC CGTCTC(1/5)
BspDI 2052 ATCGAT AT^CGAT
BspEI 1045 TCCGGA T^CCGGA
BssSI 907 CACGAG CACGAG(-5/-1)
Bst1107I 521 GTATAC GTA^TAC
BstXI 2665 CCACATGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 521 GTATAC GTA^TAC
ClaI 2052 ATCGAT AT^CGAT
Eam1105I 5294 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 4957 ATGCAT ATGCA^T
KasI 3402 GGCGCC G^GCGCC
KspI 3736 CCGCGG CCGC^GG
MroI 1045 TCCGGA T^CCGGA
NarI 3402 GGCGCC GG^CGCC
NdeI 4756 CATATG CA^TATG
NsiI 4957 ATGCAT ATGCA^T
PshBI 4731 ATTAAT AT^TAAT
SacII 3736 CCGCGG CCGC^GG
SbfI 4599 CCTGCAGG CCTGCA^GG
SfoI 3402 GGCGCC GGC^GCC
SmaI 1606 CCCGGG CCC^GGG
SphI 400 GCATGC GCATG^C
Sse8387I 4599 CCTGCAGG CCTGCA^GG
XmaCI 1606 CCCGGG C^CCGGG
XmaI 1606 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 1098 GGTACC G^GTACC
3677 GGTACC
AccI 521 GTATAC GT^[AC][GT]AC
5409 GTAGAC
AccII 2717 CGCG CG^CG
3737 CGCG
AcsI 1909 GAATTT [GA]^AATT[CT]
5143 AAATTT
AflII 3190 CTTAAG C^TTAAG
5213 CTTAAG
Alw44I 2554 GTGCAC G^TGCAC
4278 GTGCAC
AocI 943 CCTGAGG CC^T[ACGT]AGG
5262 CCTGAGG
ApaLI 2554 GTGCAC G^TGCAC
4278 GTGCAC
ApoI 1909 GAATTT [GA]^AATT[CT]
5143 AAATTT
Asp718I 1098 GGTACC G^GTACC
3677 GGTACC
AspI 799 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3025 GACAGAGTC
AviII 590 TGCGCA TGC^GCA
3018 TGCGCA
BbrPI 111 CACGTG CAC^GTG
2358 CACGTG
BbsI 5468 GAAGAC GAAGAC(2/6)
805 GTCTTC
BcgI 3265 CGAGGGCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4363 GCACAAGCTTCG
BciVI 2764 GGATAC GTATCC(6/5)
3337 GGATAC
BfrI 3190 CTTAAG C^TTAAG
5213 CTTAAG
BpuAI 5468 GAAGAC GAAGAC(2/6)
805 GTCTTC
BsaAI 111 CACGTG [CT]AC^GT[GA]
2358 CACGTG
BsaWI 1045 TCCGGA [AT]^CCGG[AT]
2700 ACCGGA
Bsp1407I 1077 TGTACA T^GTACA
3345 TGTACA
BspLU11I 2465 ACATGT A^CATGT
3879 ACATGT
BsrBI 2372 CCGCTC CCGCTC(-3/-3)
5304 CCGCTC
BsrDI 1344 CATTGC GCAATG(2/0)
2265 GCAATG
BsrGI 1077 TGTACA T^GTACA
3345 TGTACA
BstEII 1610 GGTCACC G^GT[ACGT]ACC
4537 GGTTACC
BstPI 1610 GGTCACC G^GT[ACGT]ACC
4537 GGTTACC
BstUI 2717 CGCG CG^CG
3737 CGCG
Bsu36I 943 CCTGAGG CC^T[ACGT]AGG
5262 CCTGAGG
DrdI 2103 GACCCTACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4262 GACTTCAATGTC
Eco81I 943 CCTGAGG CC^T[ACGT]AGG
5262 CCTGAGG
EcoNI 1280 CCTGGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4099 CCTGGGCAAGG
EcoO65I 1610 GGTCACC G^GT[ACGT]ACC
4537 GGTTACC
FspI 590 TGCGCA TGC^GCA
3018 TGCGCA
HincII 796 GTTGAC GT[CT]^[GA]AC
3479 GTCAAC
HindII 796 GTTGAC GT[CT]^[GA]AC
3479 GTCAAC
HindIII 4367 AAGCTT A^AGCTT
4626 AAGCTT
KpnI 1098 GGTACC GGTAC^C
3677 GGTACC
MvnI 2717 CGCG CG^CG
3737 CGCG
PflFI 799 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3025 GACAGAGTC
PmaCI 111 CACGTG CAC^GTG
2358 CACGTG
PmlI 111 CACGTG CAC^GTG
2358 CACGTG
SspBI 1077 TGTACA T^GTACA
3345 TGTACA
Tth111I 799 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3025 GACAGAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AciI AcyI AfaI AflIII AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbvCI
BbvI BclI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI EcoO109I EcoRII EcoRV EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII