##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI AhdI Alw44I ApaLI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BamHI BbeI BbrPI BcgI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaWI BseAI BsiWI Bsp1407I
BspDI BspEI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstXI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco52I
FseI FspI HpaI KasI KpnI
KspI MluI MroI NaeI NarI
NgoMIV NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I RsrII SacII
SalI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI SspBI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AflII 2075 CTTAAG C^TTAAG
AlwNI 4207 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1145 CCTGAGG CC^T[ACGT]AGG
Aor51HI 3508 AGCGCT AGC^GCT
AspI 3103 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 573 CCCGGG C^[CT]CG[GA]G
BanI 238 GGCACC G^G[CT][GA]CC
BbvCI 2059 GCTGAGG CCTCAGC(-5/-2)
BciVI 2178 GTATCC GTATCC(6/5)
BclI 4000 TGATCA T^GATCA
BfrI 2075 CTTAAG C^TTAAG
BsaBI 3318 GATTCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 323 GAGGAG GAGGAG(10/8)
BsgI 506 GTGCAG GTGCAG(16/14)
BsoBI 573 CCCGGG C^[CT]CG[GA]G
BsrBI 1242 GAGCGG CCGCTC(-3/-3)
BstAPI 2486 GCATGTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 538 GGTCACC G^GT[ACGT]ACC
BstPI 538 GGTCACC G^GT[ACGT]ACC
BstYI 838 GGATCT [GA]^GATC[CT]
Bsu36I 1145 CCTGAGG CC^T[ACGT]AGG
DraIII 4175 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 3508 AGCGCT AGC^GCT
Eco81I 1145 CCTGAGG CC^T[ACGT]AGG
EcoO65I 538 GGTCACC G^GT[ACGT]ACC
EcoRV 176 GATATC GAT^ATC
FauI 3539 CCCGC CCCGC(4/6)
FbaI 4000 TGATCA T^GATCA
HaeII 3508 AGCGCT [GA]GCGC^[CT]
HincII 3831 GTTGAC GT[CT]^[GA]AC
HindII 3831 GTTGAC GT[CT]^[GA]AC
HindIII 1867 AAGCTT A^AGCTT
MamI 3318 GATTCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3766 CAATTG C^AATTG
MflI 838 GGATCT [GA]^GATC[CT]
MspA1I 123 CAGCTG C[AC]G^C[GT]G
MunI 3766 CAATTG C^AATTG
NcoI 3880 CCATGG C^CATGG
NheI 2206 GCTAGC G^CTAGC
PflFI 3103 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 1182 CGATCG CGAT^CG
PvuII 123 CAGCTG CAG^CTG
SbfI 4203 CCTGCAGG CCTGCA^GG
SmaI 573 CCCGGG CCC^GGG
Sse8387I 4203 CCTGCAGG CCTGCA^GG
StuI 2370 AGGCCT AGG^CCT
Tth111I 3103 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 838 GGATCT [GA]^GATC[CT]
XmaCI 573 CCCGGG C^CCGGG
XmaI 573 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 702 CGCG CG^CG
713 CGCG
AcyI 1754 GACGCC G[GA]^CG[CT]C
2744 GACGCC
AflIII 290 ACATGT A^C[GA][CT]GT
1570 ACATGT
AlwI 2431 GATCC GGATC(4/5)
838 GGATC
BsaHI 1754 GACGCC G[GA]^CG[CT]C
2744 GACGCC
BsiEI 921 CGACCG CG[GA][CT]^CG
1182 CGATCG
BsmBI 1080 GAGACG CGTCTC(1/5)
1752 GAGACG
BspHI 1332 TCATGA T^CATGA
3285 TCATGA
BspLU11I 290 ACATGT A^CATGT
1570 ACATGT
BstUI 702 CGCG CG^CG
713 CGCG
EcoNI 221 CCTCTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3562 CCTTCTGCAGG
EcoRI 622 GAATTC G^AATTC
3056 GAATTC
EcoT22I 1225 ATGCAT ATGCA^T
2160 ATGCAT
HapII 574 CCGG C^CGG
1324 CCGG
HgaI 1754 GACGC GACGC(5/10)
2744 GACGC
Hin1I 1754 GACGCC G[GA]^CG[CT]C
2744 GACGCC
HpaII 574 CCGG C^CGG
1324 CCGG
MaeII 942 ACGT A^CGT
1083 ACGT
MspI 574 CCGG C^CGG
1324 CCGG
MvnI 702 CGCG CG^CG
713 CGCG
NsiI 1225 ATGCAT ATGCA^T
2160 ATGCAT
RcaI 1332 TCATGA T^CATGA
3285 TCATGA
SacI 1290 GAGCTC GAGCT^C
2653 GAGCTC
SapI 1283 GAAGAGC GCTCTTC(1/4)
64 GCTCTTC
SspI 3455 AATATT AAT^ATT
4040 AATATT
TaqI 920 TCGA T^CGA
1173 TCGA
TthHB8I 920 TCGA T^CGA
1173 TCGA
XbaI 1538 TCTAGA T^CTAGA
2766 TCTAGA
XcmI 1453 CCAGCCTGAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1673 CCAACCAAGCCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AcsI AfaI AluI ApaI
ApoI Asp700I AspHI AvaII BalI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MvaI MwoI
NciI NdeI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I XmnI