################################## # NO CUT ENZYMES ( 87 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp718I AspEI AviII AvrII BamHI BbeI BbrPI BcgI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaWI BseAI BsiWI Bsp1407I BspDI BspEI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco52I FseI FspI HpaI KasI KpnI KspI MluI MroI NaeI NarI NgoMIV NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I RsrII SacII SalI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI SspBI SwaI XhoI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AflII 2075 CTTAAG C^TTAAG AlwNI 4207 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1145 CCTGAGG CC^T[ACGT]AGG Aor51HI 3508 AGCGCT AGC^GCT AspI 3103 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 573 CCCGGG C^[CT]CG[GA]G BanI 238 GGCACC G^G[CT][GA]CC BbvCI 2059 GCTGAGG CCTCAGC(-5/-2) BciVI 2178 GTATCC GTATCC(6/5) BclI 4000 TGATCA T^GATCA BfrI 2075 CTTAAG C^TTAAG BsaBI 3318 GATTCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 323 GAGGAG GAGGAG(10/8) BsgI 506 GTGCAG GTGCAG(16/14) BsoBI 573 CCCGGG C^[CT]CG[GA]G BsrBI 1242 GAGCGG CCGCTC(-3/-3) BstAPI 2486 GCATGTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 538 GGTCACC G^GT[ACGT]ACC BstPI 538 GGTCACC G^GT[ACGT]ACC BstYI 838 GGATCT [GA]^GATC[CT] Bsu36I 1145 CCTGAGG CC^T[ACGT]AGG DraIII 4175 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 3508 AGCGCT AGC^GCT Eco81I 1145 CCTGAGG CC^T[ACGT]AGG EcoO65I 538 GGTCACC G^GT[ACGT]ACC EcoRV 176 GATATC GAT^ATC FauI 3539 CCCGC CCCGC(4/6) FbaI 4000 TGATCA T^GATCA HaeII 3508 AGCGCT [GA]GCGC^[CT] HincII 3831 GTTGAC GT[CT]^[GA]AC HindII 3831 GTTGAC GT[CT]^[GA]AC HindIII 1867 AAGCTT A^AGCTT MamI 3318 GATTCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 3766 CAATTG C^AATTG MflI 838 GGATCT [GA]^GATC[CT] MspA1I 123 CAGCTG C[AC]G^C[GT]G MunI 3766 CAATTG C^AATTG NcoI 3880 CCATGG C^CATGG NheI 2206 GCTAGC G^CTAGC PflFI 3103 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuI 1182 CGATCG CGAT^CG PvuII 123 CAGCTG CAG^CTG SbfI 4203 CCTGCAGG CCTGCA^GG SmaI 573 CCCGGG CCC^GGG Sse8387I 4203 CCTGCAGG CCTGCA^GG StuI 2370 AGGCCT AGG^CCT Tth111I 3103 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 838 GGATCT [GA]^GATC[CT] XmaCI 573 CCCGGG C^CCGGG XmaI 573 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccII 702 CGCG CG^CG 713 CGCG AcyI 1754 GACGCC G[GA]^CG[CT]C 2744 GACGCC AflIII 290 ACATGT A^C[GA][CT]GT 1570 ACATGT AlwI 2431 GATCC GGATC(4/5) 838 GGATC BsaHI 1754 GACGCC G[GA]^CG[CT]C 2744 GACGCC BsiEI 921 CGACCG CG[GA][CT]^CG 1182 CGATCG BsmBI 1080 GAGACG CGTCTC(1/5) 1752 GAGACG BspHI 1332 TCATGA T^CATGA 3285 TCATGA BspLU11I 290 ACATGT A^CATGT 1570 ACATGT BstUI 702 CGCG CG^CG 713 CGCG EcoNI 221 CCTCTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3562 CCTTCTGCAGG EcoRI 622 GAATTC G^AATTC 3056 GAATTC EcoT22I 1225 ATGCAT ATGCA^T 2160 ATGCAT HapII 574 CCGG C^CGG 1324 CCGG HgaI 1754 GACGC GACGC(5/10) 2744 GACGC Hin1I 1754 GACGCC G[GA]^CG[CT]C 2744 GACGCC HpaII 574 CCGG C^CGG 1324 CCGG MaeII 942 ACGT A^CGT 1083 ACGT MspI 574 CCGG C^CGG 1324 CCGG MvnI 702 CGCG CG^CG 713 CGCG NsiI 1225 ATGCAT ATGCA^T 2160 ATGCAT RcaI 1332 TCATGA T^CATGA 3285 TCATGA SacI 1290 GAGCTC GAGCT^C 2653 GAGCTC SapI 1283 GAAGAGC GCTCTTC(1/4) 64 GCTCTTC SspI 3455 AATATT AAT^ATT 4040 AATATT TaqI 920 TCGA T^CGA 1173 TCGA TthHB8I 920 TCGA T^CGA 1173 TCGA XbaI 1538 TCTAGA T^CTAGA 2766 TCTAGA XcmI 1453 CCAGCCTGAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1673 CCAACCAAGCCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 92 enzymes) # ######################################### AciI AcsI AfaI AluI ApaI ApoI Asp700I AspHI AvaII BalI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MluNI MnlI MscI MseI MslI MvaI MwoI NciI NdeI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XmnI