##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
Acc65I AccIII AclI AhdI AscI
Asp718I AspEI AviII AvrII BamHI
BciVI BlnI BsaBI BseAI BsiEI
BsiWI BspDI BspEI BspHI BssHII
BssSI ClaI CpoI DrdI EagI
Eam1105I EclXI Eco52I EcoRI EcoRV
EcoT22I FseI FspI KpnI KspI
MamI MfeI MluI MroI MunI
NaeI NgoMIV NotI NruI NsiI
PacI PmeI Psp1406I PvuI RcaI
RsrII SacI SacII ScaI SfiI
SgrAI SnaBI SpeI SphI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 5566 GACGTC GACGT^C
AccII 11 CGCG CG^CG
AflII 2876 CTTAAG C^TTAAG
AgeI 1566 ACCGGT A^CCGGT
Aor51HI 1469 AGCGCT AGC^GCT
ApaI 1028 GGGCCC GGGCC^C
AseI 2373 ATTAAT AT^TAAT
AsnI 2373 ATTAAT AT^TAAT
AspI 896 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 4310 GGCGCC GGCGC^C
BcgI 1442 GCAGCTAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4865 TGATCA T^GATCA
BfrI 2876 CTTAAG C^TTAAG
BglII 4537 AGATCT A^GATCT
BsmBI 1864 CGTCTC CGTCTC(1/5)
BspLU11I 5216 ACATGT A^CATGT
BsrFI 1566 ACCGGT [GA]^CCGG[CT]
Bst1107I 3115 GTATAC GTA^TAC
BstAPI 2358 GCATACAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 5053 TTCGAA TT^CGAA
BstEII 2038 GGTCACC G^GT[ACGT]ACC
BstPI 2038 GGTCACC G^GT[ACGT]ACC
BstUI 11 CGCG CG^CG
BstYI 4537 AGATCT [GA]^GATC[CT]
BstZ17I 3115 GTATAC GTA^TAC
Cfr10I 1566 ACCGGT [GA]^CCGG[CT]
Eco47III 1469 AGCGCT AGC^GCT
EcoO65I 2038 GGTCACC G^GT[ACGT]ACC
FbaI 4865 TGATCA T^GATCA
HindIII 3353 AAGCTT A^AGCTT
HpaI 5589 GTTAAC GTT^AAC
KasI 4310 GGCGCC G^GCGCC
MflI 4537 AGATCT [GA]^GATC[CT]
MvnI 11 CGCG CG^CG
NarI 4310 GGCGCC GG^CGCC
NdeI 5180 CATATG CA^TATG
NheI 1445 GCTAGC G^CTAGC
NspV 5053 TTCGAA TT^CGAA
PaeR7I 1397 CTCGAG C^TCGAG
PflFI 896 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 560 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1566 ACCGGT A^CCGGT
PshAI 1083 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2373 ATTAAT AT^TAAT
SalI 2274 GTCGAC G^TCGAC
SbfI 1833 CCTGCAGG CCTGCA^GG
SfoI 4310 GGCGCC GGC^GCC
SfuI 5053 TTCGAA TT^CGAA
Sse8387I 1833 CCTGCAGG CCTGCA^GG
Tth111I 896 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 560 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1397 CTCGAG C^TCGAG
XhoII 4537 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 3481 ACGTGT A^C[GA][CT]GT
5216 ACATGT
Alw44I 1266 GTGCAC G^TGCAC
2811 GTGCAC
ApaLI 1266 GTGCAC G^TGCAC
2811 GTGCAC
Asp700I 3515 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3948 GAAGCACTTC
BalI 1858 TGGCCA TGG^CCA
2195 TGGCCA
BbrPI 498 CACGTG CAC^GTG
4369 CACGTG
BbsI 842 GAAGAC GAAGAC(2/6)
1070 GTCTTC
BbvCI 1791 CCTCAGC CCTCAGC(-5/-2)
4168 CCTCAGC
BglI 177 GCCCTGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2079 GCCCCCCTGGC
BlpI 101 GCTCAGC GC^T[ACGT]AGC
725 GCTGAGC
Bpu1102I 101 GCTCAGC GC^T[ACGT]AGC
725 GCTGAGC
BpuAI 842 GAAGAC GAAGAC(2/6)
1070 GTCTTC
BsaAI 498 CACGTG [CT]AC^GT[GA]
4369 CACGTG
BsaI 160 GAGACC GGTCTC(1/5)
4898 GAGACC
BsaWI 1566 ACCGGT [AT]^CCGG[AT]
2470 ACCGGA
BsrBI 480 GAGCGG CCGCTC(-3/-3)
4306 GAGCGG
BstXI 157 CCAGAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
868 CCATATCTGTGG
CelII 101 GCTCAGC GC^T[ACGT]AGC
725 GCTGAGC
DraI 1571 TTTAAA TTT^AAA
2954 TTTAAA
DraIII 1282 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
3859 CACCCTGTG
MluNI 1858 TGGCCA TGG^CCA
2195 TGGCCA
MscI 1858 TGGCCA TGG^CCA
2195 TGGCCA
NcoI 2573 CCATGG C^CATGG
3338 CCATGG
PmaCI 498 CACGTG CAC^GTG
4369 CACGTG
PmlI 498 CACGTG CAC^GTG
4369 CACGTG
SspI 4070 AATATT AAT^ATT
5635 AATATT
StuI 1304 AGGCCT AGG^CCT
4837 AGGCCT
XmnI 3515 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3948 GAAGCACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BfaI BmyI BpmI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspMI BsrDI
BsrGI BsrI BssKI BstF5I BstNI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmaI SmlI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XmaCI
XmaI