##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AccI AccII AccIII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BbvCI BciVI BclI
BglII BlnI BsaAI BseAI BsgI
BsiWI Bsp1407I BspEI BspLU11I BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstUI BstZ17I Cfr10I CpoI DrdI
Eam1105I Eco47III EcoNI EcoRI EcoRV
FbaI FseI FspI HgaI HincII
HindII HindIII HpaI KasI KspI
MfeI MluI MroI MslI MspA1I
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI PvuI PvuII
RsrII SacII SalI SbfI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI Tth111I
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AatII 51 GACGTC GACGT^C
Acc65I 1239 GGTACC G^GTACC
AclI 436 AACGTT AA^CGTT
AcyI 51 GACGTC G[GA]^CG[CT]C
AocI 1269 CCTCAGG CC^T[ACGT]AGG
Asp718I 1239 GGTACC G^GTACC
AspHI 905 GAGCTC G[AT]GC[AT]^C
BalI 831 TGGCCA TGG^CCA
BcgI 812 GCATTTACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 665 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 665 GCTGAGC GC^T[ACGT]AGC
BsaBI 829 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 51 GACGTC G[GA]^CG[CT]C
BsaI 746 GAGACC GGTCTC(1/5)
BsaWI 10 ACCGGA [AT]^CCGG[AT]
BseRI 525 GAGGAG GAGGAG(10/8)
BsiEI 346 CGGCCG CG[GA][CT]^CG
BsiHKAI 905 GAGCTC G[AT]GC[AT]^C
BsmBI 53 CGTCTC CGTCTC(1/5)
BsmI 711 GAATGC GAATGC(1/-1)
BsrDI 585 GCAATG GCAATG(2/0)
BssSI 499 CTCGTG CACGAG(-5/-1)
BstEII 6 GGTGACC G^GT[ACGT]ACC
BstPI 6 GGTGACC G^GT[ACGT]ACC
BstXI 1034 CCAGCTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1269 CCTCAGG CC^T[ACGT]AGG
CelII 665 GCTGAGC GC^T[ACGT]AGC
CfoI 282 GCGC GCG^C
DraI 444 TTTAAA TTT^AAA
DraIII 1123 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 346 CGGCCG C^GGCCG
EclXI 346 CGGCCG C^GGCCG
Eco52I 346 CGGCCG C^GGCCG
Eco81I 1269 CCTCAGG CC^T[ACGT]AGG
EcoO65I 6 GGTGACC G^GT[ACGT]ACC
EcoT22I 1092 ATGCAT ATGCA^T
HaeII 281 GGCGCT [GA]GCGC^[CT]
HhaI 282 GCGC GCG^C
Hin1I 51 GACGTC G[GA]^CG[CT]C
HinP1I 282 GCGC G^CGC
KpnI 1239 GGTACC GGTAC^C
MamI 829 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 831 TGGCCA TGG^CCA
MscI 831 TGGCCA TGG^CCA
NsiI 1092 ATGCAT ATGCA^T
NspI 1090 GCATGC [GA]CATG^[CT]
PflMI 1257 CCAACCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 867 GACCCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 436 AACGTT AA^CGTT
SacI 905 GAGCTC GAGCT^C
SapI 907 GCTCTTC GCTCTTC(1/4)
ScaI 1507 AGTACT AGT^ACT
SexAI 1124 ACCAGGT A^CC[AT]GGT
SfiI 1048 GGCCTTTTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 869 CCCGGG CCC^GGG
SphI 1090 GCATGC GCATG^C
TfiI 400 GAATC G^A[AT]TC
Van91I 1257 CCAACCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1034 CCAGCTATGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 869 CCCGGG C^CCGGG
XmaI 869 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 792 GAATTT [GA]^AATT[CT]
1170 GAATTT
AflII 111 CTTAAG C^TTAAG
548 CTTAAG
ApoI 792 GAATTT [GA]^AATT[CT]
1170 GAATTT
BanI 980 GGTGCC G^G[CT][GA]CC
1239 GGTACC
BanII 600 GAGCCC G[GA]GC[CT]^C
905 GAGCTC
BfaI 784 CTAG C^TAG
1104 CTAG
BfrI 111 CTTAAG C^TTAAG
548 CTTAAG
BglI 670 GCCAGAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1049 GCCTTTTGGGC
BmyI 600 GAGCCC G[AGT]GC[ACT]^C
905 GAGCTC
BpmI 899 CTCCAG CTGGAG(16/14)
1201 CTCCAG
BsmFI 1147 GGGAC GGGAC(10/14)
874 GTCCC
Bsp1286I 600 GAGCCC G[AGT]GC[ACT]^C
905 GAGCTC
BspDI 125 ATCGAT AT^CGAT
1409 ATCGAT
BspHI 464 TCATGA T^CATGA
479 TCATGA
BstYI 92 GGATCT [GA]^GATC[CT]
247 AGATCC
Cac8I 184 GCTCGC GC[ACGT]^[ACGT]GC
1090 GCATGC
ClaI 125 ATCGAT AT^CGAT
1409 ATCGAT
DraII 872 GGGTCCC [GA]G^G[ACGT]CC[CT]
1147 GGGACCT
EaeI 346 CGGCCG [CT]^GGCC[GA]
831 TGGCCA
EcoO109I 872 GGGTCCC [GA]G^G[ACGT]CC[CT]
1147 GGGACCT
FauI 876 CCCGC CCCGC(4/6)
915 CCCGC
HphI 6 GGTGA GGTGA(8/7)
887 GGTGA
MaeI 784 CTAG C^TAG
1104 CTAG
MflI 92 GGATCT [GA]^GATC[CT]
247 AGATCC
NcoI 517 CCATGG C^CATGG
1262 CCATGG
PpuMI 872 GGGTCCC [GA]G^G[AT]CC[CT]
1147 GGGACCT
RcaI 464 TCATGA T^CATGA
479 TCATGA
SmlI 111 CTTAAG C^T[CT][GA]AG
548 CTTAAG
Tsp45I 7 GTGAC ^GT[GC]AC
800 GTCAC
XhoII 92 GGATCT [GA]^GATC[CT]
247 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
AvaII BbsI BbvI BcnI BpuAI
BsaJI BsiYI BslI BsmAI BsoBI
BspMI BsrBI BsrI BssKI BstF5I
BstNI Cfr13I DdeI DpnII DsaI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MseI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI Tru9I
TseI Tsp509I TspRI TthHB8I