################################## # NO CUT ENZYMES ( 103 enzymes) # ################################## AccI AccII AccIII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BamHI BbeI BbrPI BbvCI BciVI BclI BglII BlnI BsaAI BseAI BsgI BsiWI Bsp1407I BspEI BspLU11I BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstUI BstZ17I Cfr10I CpoI DrdI Eam1105I Eco47III EcoNI EcoRI EcoRV FbaI FseI FspI HgaI HincII HindII HindIII HpaI KasI KspI MfeI MluI MroI MslI MspA1I MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI PvuI PvuII RsrII SacII SalI SbfI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AatII 51 GACGTC GACGT^C Acc65I 1239 GGTACC G^GTACC AclI 436 AACGTT AA^CGTT AcyI 51 GACGTC G[GA]^CG[CT]C AocI 1269 CCTCAGG CC^T[ACGT]AGG Asp718I 1239 GGTACC G^GTACC AspHI 905 GAGCTC G[AT]GC[AT]^C BalI 831 TGGCCA TGG^CCA BcgI 812 GCATTTACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 665 GCTGAGC GC^T[ACGT]AGC Bpu1102I 665 GCTGAGC GC^T[ACGT]AGC BsaBI 829 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 51 GACGTC G[GA]^CG[CT]C BsaI 746 GAGACC GGTCTC(1/5) BsaWI 10 ACCGGA [AT]^CCGG[AT] BseRI 525 GAGGAG GAGGAG(10/8) BsiEI 346 CGGCCG CG[GA][CT]^CG BsiHKAI 905 GAGCTC G[AT]GC[AT]^C BsmBI 53 CGTCTC CGTCTC(1/5) BsmI 711 GAATGC GAATGC(1/-1) BsrDI 585 GCAATG GCAATG(2/0) BssSI 499 CTCGTG CACGAG(-5/-1) BstEII 6 GGTGACC G^GT[ACGT]ACC BstPI 6 GGTGACC G^GT[ACGT]ACC BstXI 1034 CCAGCTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1269 CCTCAGG CC^T[ACGT]AGG CelII 665 GCTGAGC GC^T[ACGT]AGC CfoI 282 GCGC GCG^C DraI 444 TTTAAA TTT^AAA DraIII 1123 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 346 CGGCCG C^GGCCG EclXI 346 CGGCCG C^GGCCG Eco52I 346 CGGCCG C^GGCCG Eco81I 1269 CCTCAGG CC^T[ACGT]AGG EcoO65I 6 GGTGACC G^GT[ACGT]ACC EcoT22I 1092 ATGCAT ATGCA^T HaeII 281 GGCGCT [GA]GCGC^[CT] HhaI 282 GCGC GCG^C Hin1I 51 GACGTC G[GA]^CG[CT]C HinP1I 282 GCGC G^CGC KpnI 1239 GGTACC GGTAC^C MamI 829 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 831 TGGCCA TGG^CCA MscI 831 TGGCCA TGG^CCA NsiI 1092 ATGCAT ATGCA^T NspI 1090 GCATGC [GA]CATG^[CT] PflMI 1257 CCAACCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 867 GACCCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 436 AACGTT AA^CGTT SacI 905 GAGCTC GAGCT^C SapI 907 GCTCTTC GCTCTTC(1/4) ScaI 1507 AGTACT AGT^ACT SexAI 1124 ACCAGGT A^CC[AT]GGT SfiI 1048 GGCCTTTTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 869 CCCGGG CCC^GGG SphI 1090 GCATGC GCATG^C TfiI 400 GAATC G^A[AT]TC Van91I 1257 CCAACCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1034 CCAGCTATGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 869 CCCGGG C^CCGGG XmaI 869 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AcsI 792 GAATTT [GA]^AATT[CT] 1170 GAATTT AflII 111 CTTAAG C^TTAAG 548 CTTAAG ApoI 792 GAATTT [GA]^AATT[CT] 1170 GAATTT BanI 980 GGTGCC G^G[CT][GA]CC 1239 GGTACC BanII 600 GAGCCC G[GA]GC[CT]^C 905 GAGCTC BfaI 784 CTAG C^TAG 1104 CTAG BfrI 111 CTTAAG C^TTAAG 548 CTTAAG BglI 670 GCCAGAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1049 GCCTTTTGGGC BmyI 600 GAGCCC G[AGT]GC[ACT]^C 905 GAGCTC BpmI 899 CTCCAG CTGGAG(16/14) 1201 CTCCAG BsmFI 1147 GGGAC GGGAC(10/14) 874 GTCCC Bsp1286I 600 GAGCCC G[AGT]GC[ACT]^C 905 GAGCTC BspDI 125 ATCGAT AT^CGAT 1409 ATCGAT BspHI 464 TCATGA T^CATGA 479 TCATGA BstYI 92 GGATCT [GA]^GATC[CT] 247 AGATCC Cac8I 184 GCTCGC GC[ACGT]^[ACGT]GC 1090 GCATGC ClaI 125 ATCGAT AT^CGAT 1409 ATCGAT DraII 872 GGGTCCC [GA]G^G[ACGT]CC[CT] 1147 GGGACCT EaeI 346 CGGCCG [CT]^GGCC[GA] 831 TGGCCA EcoO109I 872 GGGTCCC [GA]G^G[ACGT]CC[CT] 1147 GGGACCT FauI 876 CCCGC CCCGC(4/6) 915 CCCGC HphI 6 GGTGA GGTGA(8/7) 887 GGTGA MaeI 784 CTAG C^TAG 1104 CTAG MflI 92 GGATCT [GA]^GATC[CT] 247 AGATCC NcoI 517 CCATGG C^CATGG 1262 CCATGG PpuMI 872 GGGTCCC [GA]G^G[AT]CC[CT] 1147 GGGACCT RcaI 464 TCATGA T^CATGA 479 TCATGA SmlI 111 CTTAAG C^T[CT][GA]AG 548 CTTAAG Tsp45I 7 GTGAC ^GT[GC]AC 800 GTCAC XhoII 92 GGATCT [GA]^GATC[CT] 247 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AciI AfaI AluI AlwI AvaI AvaII BbsI BbvI BcnI BpuAI BsaJI BsiYI BslI BsmAI BsoBI BspMI BsrBI BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII DsaI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI Tru9I TseI Tsp509I TspRI TthHB8I