##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AatII AflII AgeI Alw44I Aor51HI
ApaLI AscI AspI AvrII BbeI
BfrI BglII BlnI BsaBI BsiWI
BsmBI BspLU11I BsrDI BssSI BstBI
CpoI Eco47III EcoRV FseI HpaI
KasI MamI MluI NarI NdeI
NheI NotI NruI NspV PacI
PflFI PflMI PinAI PmeI PshAI
PvuI RsrII SbfI ScaI SexAI
SfiI SfoI SfuI SpeI SphI
Sse8387I Tth111I Van91I
###################################
# ONE CUT ENZYMES ( 76 enzymes) #
###################################
AclI 3871 AACGTT AA^CGTT
AflIII 2589 ACGTGT A^C[GA][CT]GT
AhdI 354 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 2165 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2520 CCTGAGG CC^T[ACGT]AGG
AseI 2278 ATTAAT AT^TAAT
AsnI 2278 ATTAAT AT^TAAT
Asp700I 1000 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 354 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 3371 CACGTG CAC^GTG
BbvCI 1552 CCTCAGC CCTCAGC(-5/-2)
BcgI 1645 GCAACAGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3556 GGATAC GTATCC(6/5)
BclI 2198 TGATCA T^GATCA
BlpI 2580 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2580 GCTCAGC GC^T[ACGT]AGC
BsaI 352 GAGACC GGTCTC(1/5)
BsiEI 107 CGGCCG CG[GA][CT]^CG
BsmI 3746 GAATGC GAATGC(1/-1)
Bsp1407I 4145 TGTACA T^GTACA
BspDI 932 ATCGAT AT^CGAT
BspHI 2874 TCATGA T^CATGA
BspMI 1177 GCAGGT ACCTGC(4/8)
BsrBI 2990 CCGCTC CCGCTC(-3/-3)
BsrGI 4145 TGTACA T^GTACA
Bst1107I 1563 GTATAC GTA^TAC
BstEII 375 GGTGACC G^GT[ACGT]ACC
BstPI 375 GGTGACC G^GT[ACGT]ACC
BstZ17I 1563 GTATAC GTA^TAC
Bsu36I 2520 CCTGAGG CC^T[ACGT]AGG
CelII 2580 GCTCAGC GC^T[ACGT]AGC
ClaI 932 ATCGAT AT^CGAT
DraIII 2002 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1483 GACCAAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 107 CGGCCG C^GGCCG
Eam1105I 354 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 107 CGGCCG C^GGCCG
Eco52I 107 CGGCCG C^GGCCG
Eco81I 2520 CCTGAGG CC^T[ACGT]AGG
EcoNI 1744 CCTTCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 375 GGTGACC G^GT[ACGT]ACC
EcoRI 3201 GAATTC G^AATTC
EcoT22I 3909 ATGCAT ATGCA^T
FbaI 2198 TGATCA T^GATCA
HaeII 902 AGCGCC [GA]GCGC^[CT]
HincII 1414 GTCGAC GT[CT]^[GA]AC
HindII 1414 GTCGAC GT[CT]^[GA]AC
HindIII 197 AAGCTT A^AGCTT
MfeI 3958 CAATTG C^AATTG
MunI 3958 CAATTG C^AATTG
NaeI 3481 GCCGGC GCC^GGC
NcoI 1865 CCATGG C^CATGG
NgoMIV 3481 GCCGGC G^CCGGC
NsiI 3909 ATGCAT ATGCA^T
NspI 851 GCATGT [GA]CATG^[CT]
PaeR7I 719 CTCGAG C^TCGAG
PmaCI 3371 CACGTG CAC^GTG
PmlI 3371 CACGTG CAC^GTG
PshBI 2278 ATTAAT AT^TAAT
Psp1406I 3871 AACGTT AA^CGTT
PstI 1816 CTGCAG CTGCA^G
PvuII 1936 CAGCTG CAG^CTG
RcaI 2874 TCATGA T^CATGA
SalI 1414 GTCGAC G^TCGAC
SapI 3811 GAAGAGC GCTCTTC(1/4)
SgrAI 3480 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 44 CCCGGG CCC^GGG
SnaBI 3455 TACGTA TAC^GTA
SspBI 4145 TGTACA T^GTACA
StuI 2193 AGGCCT AGG^CCT
SwaI 3845 ATTTAAAT ATTT^AAAT
XbaI 1608 TCTAGA T^CTAGA
XhoI 719 CTCGAG C^TCGAG
XmaCI 44 CCCGGG C^CCGGG
XmaI 44 CCCGGG C^CCGGG
XmnI 1000 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 1243 GGTACC G^GTACC
1753 GGTACC
AccIII 2982 TCCGGA T^CCGGA
3020 TCCGGA
AcyI 1045 GACGCC G[GA]^CG[CT]C
2285 GACGCC
Asp718I 1243 GGTACC G^GTACC
1753 GGTACC
AviII 81 TGCGCA TGC^GCA
2924 TGCGCA
BalI 1080 TGGCCA TGG^CCA
1862 TGGCCA
BamHI 1270 GGATCC G^GATCC
2772 GGATCC
BanI 1243 GGTACC G^G[CT][GA]CC
1753 GGTACC
BbsI 1423 GAAGAC GAAGAC(2/6)
1975 GAAGAC
BglI 1717 GCCCTTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3093 GCCTCGAAGGC
BpuAI 1423 GAAGAC GAAGAC(2/6)
1975 GAAGAC
BsaAI 3371 CACGTG [CT]AC^GT[GA]
3455 TACGTA
BsaHI 1045 GACGCC G[GA]^CG[CT]C
2285 GACGCC
BsaWI 2982 TCCGGA [AT]^CCGG[AT]
3020 TCCGGA
BseAI 2982 TCCGGA T^CCGGA
3020 TCCGGA
BspEI 2982 TCCGGA T^CCGGA
3020 TCCGGA
BsrFI 2130 GCCGGT [GA]^CCGG[CT]
3481 GCCGGC
BssHII 23 GCGCGC G^CGCGC
56 GCGCGC
BstXI 2466 CCACACATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2666 CCATCGTCATGG
Cfr10I 2130 GCCGGT [GA]^CCGG[CT]
3481 GCCGGC
FspI 81 TGCGCA TGC^GCA
2924 TGCGCA
Hin1I 1045 GACGCC G[GA]^CG[CT]C
2285 GACGCC
KpnI 1243 GGTACC GGTAC^C
1753 GGTACC
KspI 3 CCGCGG CCGC^GG
38 CCGCGG
MluNI 1080 TGGCCA TGG^CCA
1862 TGGCCA
MroI 2982 TCCGGA T^CCGGA
3020 TCCGGA
MscI 1080 TGGCCA TGG^CCA
1862 TGGCCA
PpuMI 662 AGGTCCC [GA]G^G[AT]CC[CT]
1508 AGGTCCC
SacII 3 CCGCGG CCGC^GG
38 CCGCGG
SspI 920 AATATT AAT^ATT
3850 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI ApaI ApoI AspHI
AvaI AvaII BanII BbvI BcnI
BfaI BmyI BpmI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII