################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## Acc65I AflII AgeI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AviII BbeI BfrI BglII BsaBI BsiWI BssHII Bst1107I BstBI BstEII BstPI BstZ17I CpoI DrdI EagI EclXI Eco47III Eco52I EcoO65I FseI FspI HaeII HgaI HpaI KasI KpnI KspI MamI MfeI MunI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PshAI PshBI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 2361 GACGTC GACGT^C AccI 2854 GTCTAC GT^[AC][GT]AC AcyI 2361 GACGTC G[GA]^CG[CT]C AhdI 514 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 514 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2332 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 4662 CCTAGG C^CTAGG BanI 1510 GGCACC G^G[CT][GA]CC BbrPI 1192 CACGTG CAC^GTG BciVI 3819 GTATCC GTATCC(6/5) BglI 2644 GCCTGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 4662 CCTAGG C^CTAGG BlpI 2468 GCTTAGC GC^T[ACGT]AGC Bpu1102I 2468 GCTTAGC GC^T[ACGT]AGC BsaHI 2361 GACGTC G[GA]^CG[CT]C BsmBI 76 GAGACG CGTCTC(1/5) BsmI 491 GCATTC GAATGC(1/-1) BspDI 2067 ATCGAT AT^CGAT BspLU11I 4487 ACATGT A^CATGT BspMI 850 ACCTGC ACCTGC(4/8) BsrBI 1671 CCGCTC CCGCTC(-3/-3) BsrFI 2729 ACCGGC [GA]^CCGG[CT] BstXI 3499 CCAAATCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 2468 GCTTAGC GC^T[ACGT]AGC CfoI 2538 GCGC GCG^C Cfr10I 2729 ACCGGC [GA]^CCGG[CT] ClaI 2067 ATCGAT AT^CGAT Eam1105I 514 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 2106 CCTTGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 2428 GATATC GAT^ATC HhaI 2538 GCGC GCG^C Hin1I 2361 GACGTC G[GA]^CG[CT]C HinP1I 2538 GCGC G^CGC MluI 2870 ACGCGT A^CGCGT NcoI 2495 CCATGG C^CATGG PflFI 2332 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 1192 CACGTG CAC^GTG PmlI 1192 CACGTG CAC^GTG PvuI 2069 CGATCG CGAT^CG SacI 4462 GAGCTC GAGCT^C SmaI 806 CCCGGG CCC^GGG SmlI 544 CTCAAG C^T[CT][GA]AG StuI 2712 AGGCCT AGG^CCT Tth111I 2332 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 806 CCCGGG C^CCGGG XmaI 806 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccII 2871 CGCG CG^CG 2925 CGCG AccIII 368 TCCGGA T^CCGGA 1337 TCCGGA AclI 1930 AACGTT AA^CGTT 3361 AACGTT BbvCI 855 CCTCAGC CCTCAGC(-5/-2) 896 CCTCAGC BcgI 438 CGAGAATATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3044 GCAAGAGTATCG BclI 251 TGATCA T^GATCA 1586 TGATCA BsaAI 1192 CACGTG [CT]AC^GT[GA] 4307 CACGTA BsaI 3490 GAGACC GGTCTC(1/5) 2405 GGTCTC BsaWI 368 TCCGGA [AT]^CCGG[AT] 1337 TCCGGA BseAI 368 TCCGGA T^CCGGA 1337 TCCGGA BsgI 1858 CTGCAC GTGCAG(16/14) 3208 GTGCAG BsiEI 2069 CGATCG CG[GA][CT]^CG 2619 CGGTCG BspEI 368 TCCGGA T^CCGGA 1337 TCCGGA BssSI 4177 CACGAG CACGAG(-5/-1) 4574 CTCGTG BstUI 2871 CGCG CG^CG 2925 CGCG DraI 2412 TTTAAA TTT^AAA 3667 TTTAAA EcoT22I 4405 ATGCAT ATGCA^T 4409 ATGCAT FbaI 251 TGATCA T^GATCA 1586 TGATCA HindIII 837 AAGCTT A^AGCTT 3215 AAGCTT MroI 368 TCCGGA T^CCGGA 1337 TCCGGA MvnI 2871 CGCG CG^CG 2925 CGCG NdeI 4254 CATATG CA^TATG 4412 CATATG NsiI 4405 ATGCAT ATGCA^T 4409 ATGCAT PflMI 1552 CCAATCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2495 CCATGGTGTGG Psp1406I 1930 AACGTT AA^CGTT 3361 AACGTT SphI 1243 GCATGC GCATG^C 4407 GCATGC SspI 442 AATATT AAT^ATT 4203 AATATT Van91I 1552 CCAATCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2495 CCATGGTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 112 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI AspHI AvaI AvaII BalI BamHI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspHI BsrDI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstYI Bsu36I Cac8I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RcaI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII