##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
Acc65I AflII AgeI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AviII BbeI BfrI
BglII BsaBI BsiWI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I CpoI
DrdI EagI EclXI Eco47III Eco52I
EcoO65I FseI FspI HaeII HgaI
HpaI KasI KpnI KspI MamI
MfeI MunI NaeI NarI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SwaI XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 2361 GACGTC GACGT^C
AccI 2854 GTCTAC GT^[AC][GT]AC
AcyI 2361 GACGTC G[GA]^CG[CT]C
AhdI 514 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 514 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2332 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4662 CCTAGG C^CTAGG
BanI 1510 GGCACC G^G[CT][GA]CC
BbrPI 1192 CACGTG CAC^GTG
BciVI 3819 GTATCC GTATCC(6/5)
BglI 2644 GCCTGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 4662 CCTAGG C^CTAGG
BlpI 2468 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 2468 GCTTAGC GC^T[ACGT]AGC
BsaHI 2361 GACGTC G[GA]^CG[CT]C
BsmBI 76 GAGACG CGTCTC(1/5)
BsmI 491 GCATTC GAATGC(1/-1)
BspDI 2067 ATCGAT AT^CGAT
BspLU11I 4487 ACATGT A^CATGT
BspMI 850 ACCTGC ACCTGC(4/8)
BsrBI 1671 CCGCTC CCGCTC(-3/-3)
BsrFI 2729 ACCGGC [GA]^CCGG[CT]
BstXI 3499 CCAAATCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2468 GCTTAGC GC^T[ACGT]AGC
CfoI 2538 GCGC GCG^C
Cfr10I 2729 ACCGGC [GA]^CCGG[CT]
ClaI 2067 ATCGAT AT^CGAT
Eam1105I 514 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2106 CCTTGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2428 GATATC GAT^ATC
HhaI 2538 GCGC GCG^C
Hin1I 2361 GACGTC G[GA]^CG[CT]C
HinP1I 2538 GCGC G^CGC
MluI 2870 ACGCGT A^CGCGT
NcoI 2495 CCATGG C^CATGG
PflFI 2332 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1192 CACGTG CAC^GTG
PmlI 1192 CACGTG CAC^GTG
PvuI 2069 CGATCG CGAT^CG
SacI 4462 GAGCTC GAGCT^C
SmaI 806 CCCGGG CCC^GGG
SmlI 544 CTCAAG C^T[CT][GA]AG
StuI 2712 AGGCCT AGG^CCT
Tth111I 2332 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 806 CCCGGG C^CCGGG
XmaI 806 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 2871 CGCG CG^CG
2925 CGCG
AccIII 368 TCCGGA T^CCGGA
1337 TCCGGA
AclI 1930 AACGTT AA^CGTT
3361 AACGTT
BbvCI 855 CCTCAGC CCTCAGC(-5/-2)
896 CCTCAGC
BcgI 438 CGAGAATATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3044 GCAAGAGTATCG
BclI 251 TGATCA T^GATCA
1586 TGATCA
BsaAI 1192 CACGTG [CT]AC^GT[GA]
4307 CACGTA
BsaI 3490 GAGACC GGTCTC(1/5)
2405 GGTCTC
BsaWI 368 TCCGGA [AT]^CCGG[AT]
1337 TCCGGA
BseAI 368 TCCGGA T^CCGGA
1337 TCCGGA
BsgI 1858 CTGCAC GTGCAG(16/14)
3208 GTGCAG
BsiEI 2069 CGATCG CG[GA][CT]^CG
2619 CGGTCG
BspEI 368 TCCGGA T^CCGGA
1337 TCCGGA
BssSI 4177 CACGAG CACGAG(-5/-1)
4574 CTCGTG
BstUI 2871 CGCG CG^CG
2925 CGCG
DraI 2412 TTTAAA TTT^AAA
3667 TTTAAA
EcoT22I 4405 ATGCAT ATGCA^T
4409 ATGCAT
FbaI 251 TGATCA T^GATCA
1586 TGATCA
HindIII 837 AAGCTT A^AGCTT
3215 AAGCTT
MroI 368 TCCGGA T^CCGGA
1337 TCCGGA
MvnI 2871 CGCG CG^CG
2925 CGCG
NdeI 4254 CATATG CA^TATG
4412 CATATG
NsiI 4405 ATGCAT ATGCA^T
4409 ATGCAT
PflMI 1552 CCAATCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2495 CCATGGTGTGG
Psp1406I 1930 AACGTT AA^CGTT
3361 AACGTT
SphI 1243 GCATGC GCATG^C
4407 GCATGC
SspI 442 AATATT AAT^ATT
4203 AATATT
Van91I 1552 CCAATCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2495 CCATGGTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApoI AspHI
AvaI AvaII BalI BamHI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspHI BsrDI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Bsu36I Cac8I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RcaI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII