##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AccII AccIII AflII AhdI
AocI Aor51HI AscI AspEI AviII
BamHI BbeI BbrPI BciVI BfrI
BsaAI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BssHII BstUI Bsu36I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I FseI
FspI HpaI KasI KspI MfeI
MluI MroI MunI MvnI NarI
NdeI NheI NotI NruI PacI
PflMI PmaCI PmeI PmlI PshAI
PvuI RsrII SacII SbfI SexAI
SfiI SfoI SmaI SnaBI SpeI
Sse8387I SspI SwaI Van91I XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 3617 GGTACC G^GTACC
AclI 824 AACGTT AA^CGTT
AcyI 74 GGCGTC G[GA]^CG[CT]C
AgeI 2490 ACCGGT A^CCGGT
Alw44I 2486 GTGCAC G^TGCAC
ApaLI 2486 GTGCAC G^TGCAC
AseI 546 ATTAAT AT^TAAT
AsnI 546 ATTAAT AT^TAAT
Asp700I 767 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3617 GGTACC G^GTACC
AspI 1413 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BsaHI 74 GGCGTC G[GA]^CG[CT]C
Bsp1407I 4660 TGTACA T^GTACA
BspHI 4030 TCATGA T^CATGA
BsrGI 4660 TGTACA T^GTACA
BssSI 5487 CACGAG CACGAG(-5/-1)
Bst1107I 3742 GTATAC GTA^TAC
BstBI 1985 TTCGAA TT^CGAA
BstXI 5295 CCATGTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3742 GTATAC GTA^TAC
DraI 4667 TTTAAA TTT^AAA
DsaI 2122 CCATGG C^C[GA][CT]GG
EcoRV 5459 GATATC GAT^ATC
HaeII 947 AGCGCC [GA]GCGC^[CT]
HgaI 75 GCGTC GACGC(5/10)
Hin1I 74 GGCGTC G[GA]^CG[CT]C
KpnI 3617 GGTACC GGTAC^C
NcoI 2122 CCATGG C^CATGG
NspV 1985 TTCGAA TT^CGAA
PaeR7I 4915 CTCGAG C^TCGAG
PflFI 1413 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2490 ACCGGT A^CCGGT
PshBI 546 ATTAAT AT^TAAT
Psp1406I 824 AACGTT AA^CGTT
RcaI 4030 TCATGA T^CATGA
SalI 1410 GTCGAC G^TCGAC
SfuI 1985 TTCGAA TT^CGAA
SgrAI 2489 CACCGGTG C[GA]^CCGG[CT]G
SspBI 4660 TGTACA T^GTACA
Tth111I 1413 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 4915 CTCGAG C^TCGAG
XmnI 767 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 1410 GTCGAC GT^[AC][GT]AC
3742 GTATAC
AflIII 3018 ACATGT A^C[GA][CT]GT
4092 ACATGT
AvaI 3719 CTCGGG C^[CT]CG[GA]G
4915 CTCGAG
AvrII 2448 CCTAGG C^CTAGG
4501 CCTAGG
BalI 2423 TGGCCA TGG^CCA
5191 TGGCCA
BcgI 4400 CGACCTACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5435 GCATGAGGCTCG
BclI 727 TGATCA T^GATCA
4842 TGATCA
BlnI 2448 CCTAGG C^CTAGG
4501 CCTAGG
BsaBI 2390 GATTACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4318 GATAACTATC
BsaWI 2490 ACCGGT [AT]^CCGG[AT]
3464 TCCGGT
BsoBI 3719 CTCGGG C^[CT]CG[GA]G
4915 CTCGAG
BspLU11I 3018 ACATGT A^CATGT
4092 ACATGT
BsrBI 4435 CCGCTC CCGCTC(-3/-3)
3197 GAGCGG
BstEII 4574 GGTGACC G^GT[ACGT]ACC
4593 GGTTACC
BstPI 4574 GGTGACC G^GT[ACGT]ACC
4593 GGTTACC
CfoI 948 GCGC GCG^C
2348 GCGC
DraIII 96 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
4002 CACATGGTG
EcoNI 46 CCTGCTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
740 CCTATCACAGG
EcoO65I 4574 GGTGACC G^GT[ACGT]ACC
4593 GGTTACC
EcoRI 2037 GAATTC G^AATTC
4034 GAATTC
FbaI 727 TGATCA T^GATCA
4842 TGATCA
HhaI 948 GCGC GCG^C
2348 GCGC
HinP1I 948 GCGC G^CGC
2348 GCGC
HincII 1218 GTCAAC GT[CT]^[GA]AC
1410 GTCGAC
HindII 1218 GTCAAC GT[CT]^[GA]AC
1410 GTCGAC
MamI 2390 GATTACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4318 GATAACTATC
MluNI 2423 TGGCCA TGG^CCA
5191 TGGCCA
MscI 2423 TGGCCA TGG^CCA
5191 TGGCCA
SapI 4544 GCTCTTC GCTCTTC(1/4)
5323 GCTCTTC
ScaI 270 AGTACT AGT^ACT
2209 AGTACT
SmlI 613 CTCAAG C^T[CT][GA]AG
4915 CTCGAG
SphI 1954 GCATGC GCATG^C
2816 GCATGC
StuI 116 AGGCCT AGG^CCT
2464 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApaI ApoI AspHI AvaII
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspMI BsrDI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
CelII Cfr10I Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeIII HapII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NsiI NspI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII