##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
AhdI AocI AscI Asp718I AspEI
AvrII BanI BbeI BbrPI BcgI
BglI BglII BlnI BsaAI BsaHI
BsaWI BseAI BseRI BsiWI Bsp1407I
BspDI BspEI BsrDI BsrFI BsrGI
BssSI Bst1107I BstBI BstZ17I Bsu36I
Cfr10I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoRV EcoT22I FseI Hin1I HpaI
KasI KpnI KspI MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PvuI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I SspBI XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AclI 689 AACGTT AA^CGTT
AflII 4428 CTTAAG C^TTAAG
ApaI 162 GGGCCC GGGCC^C
Asp700I 3855 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 476 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2203 CCCGAG C^[CT]CG[GA]G
AviII 2588 TGCGCA TGC^GCA
BalI 459 TGGCCA TGG^CCA
BbvCI 1104 GCTGAGG CCTCAGC(-5/-2)
BfrI 4428 CTTAAG C^TTAAG
BsaI 3556 GGTCTC GGTCTC(1/5)
BsiEI 917 CGACCG CG[GA][CT]^CG
BsmI 3042 GCATTC GAATGC(1/-1)
BsoBI 2203 CCCGAG C^[CT]CG[GA]G
BspHI 1423 TCATGA T^CATGA
BsrBI 537 GAGCGG CCGCTC(-3/-3)
BssHII 69 GCGCGC G^CGCGC
BstAPI 2824 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 3582 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 1081 GAATTC G^AATTC
FspI 2588 TGCGCA TGC^GCA
HincII 2229 GTTGAC GT[CT]^[GA]AC
HindII 2229 GTTGAC GT[CT]^[GA]AC
HindIII 4257 AAGCTT A^AGCTT
MluNI 459 TGGCCA TGG^CCA
MscI 459 TGGCCA TGG^CCA
NheI 53 GCTAGC G^CTAGC
PflFI 476 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2954 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 3144 GACACTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 689 AACGTT AA^CGTT
PstI 1479 CTGCAG CTGCA^G
RcaI 1423 TCATGA T^CATGA
SphI 1932 GCATGC GCATG^C
SwaI 1142 ATTTAAAT ATTT^AAAT
Tth111I 476 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 3855 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 3115 GTAGAC GT^[AC][GT]AC
4503 GTCTAC
Alw44I 1390 GTGCAC G^TGCAC
2269 GTGCAC
AlwNI 2239 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2825 CAGCAGCTG
Aor51HI 56 AGCGCT AGC^GCT
584 AGCGCT
ApaLI 1390 GTGCAC G^TGCAC
2269 GTGCAC
BanII 162 GGGCCC G[GA]GC[CT]^C
2200 GAGCCC
BclI 847 TGATCA T^GATCA
3382 TGATCA
BlpI 261 GCTCAGC GC^T[ACGT]AGC
703 GCTCAGC
Bpu1102I 261 GCTCAGC GC^T[ACGT]AGC
703 GCTCAGC
BsaBI 512 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1257 GATATTTATC
BstEII 553 GGTGACC G^GT[ACGT]ACC
1152 GGTGACC
BstPI 553 GGTGACC G^GT[ACGT]ACC
1152 GGTGACC
BstXI 728 CCACAAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1090 CCATTATTTTGG
CelII 261 GCTCAGC GC^T[ACGT]AGC
703 GCTCAGC
DraI 1143 TTTAAA TTT^AAA
2147 TTTAAA
Eco47III 56 AGCGCT AGC^GCT
584 AGCGCT
EcoO65I 553 GGTGACC G^GT[ACGT]ACC
1152 GGTGACC
FbaI 847 TGATCA T^GATCA
3382 TGATCA
HaeII 56 AGCGCT [GA]GCGC^[CT]
584 AGCGCT
HgaI 3083 GACGC GACGC(5/10)
13 GCGTC
MamI 512 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1257 GATATTTATC
NcoI 448 CCATGG C^CATGG
1157 CCATGG
PflMI 24 CCAGATAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1277 CCACATCCTGG
SapI 316 GAAGAGC GCTCTTC(1/4)
4107 GCTCTTC
SpeI 3709 ACTAGT A^CTAGT
4029 ACTAGT
StuI 1418 AGGCCT AGG^CCT
2297 AGGCCT
Van91I 24 CCAGATAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1277 CCACATCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI AseI AsnI
AspHI AvaII BamHI BbsI BbvI
BciVI BcnI BfaI BmyI BpmI
BpuAI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI Bsp1286I
BspLU11I BspMI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PshBI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII