##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AccII AccIII AclI AflII AflIII
AocI ApaI AscI Asp700I BamHI
BfrI BseAI BsiWI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrGI
BssHII Bst1107I BstUI BstXI BstZ17I
Bsu36I ClaI CpoI DrdI Eco81I
EcoRV FseI KspI MfeI MluI
MroI MunI MvnI NaeI NgoMIV
NotI NruI PacI PmeI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SfiI SgrAI SnaBI Sse8387I
SspBI SwaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 5718 GTCTAC GT^[AC][GT]AC
AgeI 1156 ACCGGT A^CCGGT
AhdI 3504 GACAAGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 5728 AGCGCT AGC^GCT
AspEI 3504 GACAAGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1361 TGCGCA TGC^GCA
AvrII 4499 CCTAGG C^CTAGG
BcgI 820 GCAGGCCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 5513 TGATCA T^GATCA
BglI 462 GCCCACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2175 AGATCT A^GATCT
BlnI 4499 CCTAGG C^CTAGG
BsaBI 2198 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1156 ACCGGT [AT]^CCGG[AT]
BsiEI 419 CGGCCG CG[GA][CT]^CG
BsmBI 2042 CGTCTC CGTCTC(1/5)
BssSI 1423 CTCGTG CACGAG(-5/-1)
BstAPI 4940 GCACCTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 961 TTCGAA TT^CGAA
BstEII 4722 GGTCACC G^GT[ACGT]ACC
BstPI 4722 GGTCACC G^GT[ACGT]ACC
EagI 419 CGGCCG C^GGCCG
Eam1105I 3504 GACAAGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 419 CGGCCG C^GGCCG
Eco47III 5728 AGCGCT AGC^GCT
Eco52I 419 CGGCCG C^GGCCG
EcoO65I 4722 GGTCACC G^GT[ACGT]ACC
FbaI 5513 TGATCA T^GATCA
FspI 1361 TGCGCA TGC^GCA
HindIII 5168 AAGCTT A^AGCTT
HpaI 5687 GTTAAC GTT^AAC
MamI 2198 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI 1961 GCTAGC G^CTAGC
NspV 961 TTCGAA TT^CGAA
PaeR7I 835 CTCGAG C^TCGAG
PinAI 1156 ACCGGT A^CCGGT
SacI 3089 GAGCTC GAGCT^C
SexAI 38 ACCAGGT A^CC[AT]GGT
SfuI 961 TTCGAA TT^CGAA
SmaI 732 CCCGGG CCC^GGG
XbaI 2814 TCTAGA T^CTAGA
XhoI 835 CTCGAG C^TCGAG
XmaCI 732 CCCGGG C^CCGGG
XmaI 732 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AatII 3509 GACGTC GACGT^C
3649 GACGTC
Alw44I 678 GTGCAC G^TGCAC
898 GTGCAC
ApaLI 678 GTGCAC G^TGCAC
898 GTGCAC
AspI 2400 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4318 GACACTGTC
BbeI 1368 GGCGCC GGCGC^C
4240 GGCGCC
BspHI 2856 TCATGA T^CATGA
5534 TCATGA
BspMI 1088 ACCTGC ACCTGC(4/8)
5325 ACCTGC
DraIII 694 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
901 CACCCAGTG
EcoRI 3363 GAATTC G^AATTC
5216 GAATTC
FauI 927 CCCGC CCCGC(4/6)
4274 CCCGC
HgaI 831 GACGC GACGC(5/10)
5464 GACGC
HincII 78 GTCAAC GT[CT]^[GA]AC
5687 GTTAAC
HindII 78 GTCAAC GT[CT]^[GA]AC
5687 GTTAAC
KasI 1368 GGCGCC G^GCGCC
4240 GGCGCC
MspA1I 3075 CAGCTG C[AC]G^C[GT]G
3255 CAGCTG
NarI 1368 GGCGCC GG^CGCC
4240 GGCGCC
NcoI 658 CCATGG C^CATGG
4106 CCATGG
PflFI 2400 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4318 GACACTGTC
PvuII 3075 CAGCTG CAG^CTG
3255 CAGCTG
RcaI 2856 TCATGA T^CATGA
5534 TCATGA
SapI 3144 GAAGAGC GCTCTTC(1/4)
2286 GCTCTTC
SfoI 1368 GGCGCC GGC^GCC
4240 GGCGCC
SmlI 835 CTCGAG C^T[CT][GA]AG
1849 CTTGAG
SpeI 1026 ACTAGT A^CTAGT
2165 ACTAGT
SspI 2938 AATATT AAT^ATT
5609 AATATT
Tth111I 2400 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4318 GACACTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
Acc65I AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AseI
AsnI Asp718I AspHI AvaI AvaII
BalI BanI BanII BbrPI BbsI
BbvCI BbvI BciVI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I EcoT22I Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KpnI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspI MvaI MwoI
NciI NdeI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI PmaCI
PmlI PpuMI PshAI PshBI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SphI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII