##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII Acc65I AccI AgeI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
BglI BsiWI BspHI BspLU11I Bst1107I
BstBI BstXI BstZ17I CpoI Eco47III
EcoRI EcoRV FseI HpaI KpnI
KspI MfeI MluI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NspV PacI PinAI PmeI PvuI
RcaI RsrII SacII SalI SbfI
SfiI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SwaI XbaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AflII 756 CTTAAG C^TTAAG
AhdI 2031 GACACAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 4397 CCTCAGG CC^T[ACGT]AGG
AseI 980 ATTAAT AT^TAAT
AsnI 980 ATTAAT AT^TAAT
AspEI 2031 GACACAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3201 TGCGCA TGC^GCA
BalI 4116 TGGCCA TGG^CCA
BbrPI 4348 CACGTG CAC^GTG
BfrI 756 CTTAAG C^TTAAG
BsaBI 2733 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 3296 GAGACG CGTCTC(1/5)
BspDI 1668 ATCGAT AT^CGAT
BstAPI 1566 GCAGTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 4679 GGTGACC G^GT[ACGT]ACC
BstPI 4679 GGTGACC G^GT[ACGT]ACC
Bsu36I 4397 CCTCAGG CC^T[ACGT]AGG
ClaI 1668 ATCGAT AT^CGAT
DrdI 2671 GACTTCTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2031 GACACAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 4397 CCTCAGG CC^T[ACGT]AGG
EcoNI 558 CCTTTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 4679 GGTGACC G^GT[ACGT]ACC
EcoT22I 3567 ATGCAT ATGCA^T
FspI 3201 TGCGCA TGC^GCA
MamI 2733 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 4116 TGGCCA TGG^CCA
MscI 4116 TGGCCA TGG^CCA
NsiI 3567 ATGCAT ATGCA^T
PmaCI 4348 CACGTG CAC^GTG
PmlI 4348 CACGTG CAC^GTG
PshBI 980 ATTAAT AT^TAAT
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AclI 2130 AACGTT AA^CGTT
3765 AACGTT
AflIII 4347 ACACGT A^C[GA][CT]GT
4349 ACGTGT
AvrII 1055 CCTAGG C^CTAGG
2666 CCTAGG
BamHI 415 GGATCC G^GATCC
1586 GGATCC
BbeI 15 GGCGCC GGCGC^C
1358 GGCGCC
BbvCI 5118 CCTCAGC CCTCAGC(-5/-2)
4973 GCTGAGG
BcgI 4885 CGACATGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2873 GCATGCTGCTCG
BciVI 3812 GGATAC GTATCC(6/5)
2174 GTATCC
BclI 2738 TGATCA T^GATCA
4695 TGATCA
BlnI 1055 CCTAGG C^CTAGG
2666 CCTAGG
BlpI 1593 GCTAAGC GC^T[ACGT]AGC
3608 GCTCAGC
Bpu1102I 1593 GCTAAGC GC^T[ACGT]AGC
3608 GCTCAGC
BspMI 1770 ACCTGC ACCTGC(4/8)
2233 ACCTGC
CelII 1593 GCTAAGC GC^T[ACGT]AGC
3608 GCTCAGC
EagI 67 CGGCCG C^GGCCG
2648 CGGCCG
EclXI 67 CGGCCG C^GGCCG
2648 CGGCCG
Eco52I 67 CGGCCG C^GGCCG
2648 CGGCCG
FbaI 2738 TGATCA T^GATCA
4695 TGATCA
KasI 15 GGCGCC G^GCGCC
1358 GGCGCC
NarI 15 GGCGCC GG^CGCC
1358 GGCGCC
PshAI 1492 GACCAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4371 GACAGAGGTC
Psp1406I 2130 AACGTT AA^CGTT
3765 AACGTT
SacI 2406 GAGCTC GAGCT^C
3045 GAGCTC
SapI 3042 GAAGAGC GCTCTTC(1/4)
2417 GCTCTTC
ScaI 493 AGTACT AGT^ACT
2566 AGTACT
SexAI 1397 ACCAGGT A^CC[AT]GGT
4458 ACCTGGT
SfoI 15 GGCGCC GGC^GCC
1358 GGCGCC
SphI 2873 GCATGC GCATG^C
4735 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 142 enzymes) #
#########################################
AccII AccIII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApoI
Asp700I AspHI AspI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspEI BsrBI
BsrDI BsrFI BsrGI BsrI BssHII
BssKI BssSI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MroI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PaeR7I PflFI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoI
XhoII XmnI