##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
Acc65I AflII AflIII AgeI AscI
Asp700I Asp718I AspI AvrII BciVI
BclI BfrI BlnI BsmBI BspDI
BspHI BspLU11I Bst1107I BstEII BstPI
BstXI BstZ17I ClaI CpoI EcoO65I
EcoRV EcoT22I FbaI KpnI MfeI
MluI MunI NcoI NdeI NruI
NsiI PacI PaeR7I PflFI PinAI
PmeI PshAI PvuI RcaI RsrII
SapI ScaI SfiI SgrAI SnaBI
SpeI Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 3678 GACGTC GACGT^C
AccIII 1117 TCCGGA T^CCGGA
AclI 4177 AACGTT AA^CGTT
ApaI 243 GGGCCC GGGCC^C
AseI 2405 ATTAAT AT^TAAT
AsnI 2405 ATTAAT AT^TAAT
AviII 3283 TGCGCA TGC^GCA
BbrPI 4284 CACGTG CAC^GTG
BlpI 322 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 322 GCTGAGC GC^T[ACGT]AGC
BsaAI 4284 CACGTG [CT]AC^GT[GA]
BseAI 1117 TCCGGA T^CCGGA
BsiWI 2890 CGTACG C^GTACG
BspEI 1117 TCCGGA T^CCGGA
BsrDI 636 GCAATG GCAATG(2/0)
BstBI 4232 TTCGAA TT^CGAA
CelII 322 GCTGAGC GC^T[ACGT]AGC
DraIII 985 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 4229 GAATTC G^AATTC
FseI 228 GGCCGGCC GGCCGG^CC
FspI 3283 TGCGCA TGC^GCA
HindIII 1178 AAGCTT A^AGCTT
HpaI 4174 GTTAAC GTT^AAC
MroI 1117 TCCGGA T^CCGGA
NheI 4160 GCTAGC G^CTAGC
NotI 287 GCGGCCGC GC^GGCCGC
NspV 4232 TTCGAA TT^CGAA
PmaCI 4284 CACGTG CAC^GTG
PmlI 4284 CACGTG CAC^GTG
PshBI 2405 ATTAAT AT^TAAT
Psp1406I 4177 AACGTT AA^CGTT
SalI 3086 GTCGAC G^TCGAC
SfuI 4232 TTCGAA TT^CGAA
SspI 2402 AATATT AAT^ATT
StuI 1354 AGGCCT AGG^CCT
SwaI 3990 ATTTAAAT ATTT^AAAT
TfiI 2331 GAATC G^A[AT]TC
XbaI 4038 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 442 GTCTAC GT^[AC][GT]AC
3086 GTCGAC
Alw44I 701 GTGCAC G^TGCAC
3564 GTGCAC
Aor51HI 3460 AGCGCT AGC^GCT
3806 AGCGCT
ApaLI 701 GTGCAC G^TGCAC
3564 GTGCAC
BamHI 624 GGATCC G^GATCC
813 GGATCC
BcgI 271 CGATCATGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3566 GCACAGGCTTCG
BglII 713 AGATCT A^GATCT
3370 AGATCT
BsaBI 1394 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3650 GATGAACATC
BsmI 2316 GCATTC GAATGC(1/-1)
2559 GCATTC
Eco47III 3460 AGCGCT AGC^GCT
3806 AGCGCT
EcoNI 399 CCTGCCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2672 CCTGGCCGAGG
MamI 1394 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3650 GATGAACATC
NaeI 133 GCCGGC GCC^GGC
229 GCCGGC
NgoMIV 133 GCCGGC G^CCGGC
229 GCCGGC
SacI 1211 GAGCTC GAGCT^C
1797 GAGCTC
SbfI 1922 CCTGCAGG CCTGCA^GG
3329 CCTGCAGG
SmaI 877 CCCGGG CCC^GGG
1522 CCCGGG
SphI 771 GCATGC GCATG^C
1657 GCATGC
Sse8387I 1922 CCTGCAGG CCTGCA^GG
3329 CCTGCAGG
XcmI 628 CCAAGGGTGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1606 CCACTCGGCAGGTGG
XmaCI 877 CCCGGG C^CCGGG
1522 CCCGGG
XmaI 877 CCCGGG C^CCGGG
1522 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 144 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AhdI AluI AlwI AlwNI AocI
ApoI AspEI AspHI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BspMI BsrBI
BsrFI BsrGI BsrI BssHII BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco52I Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KasI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacII
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SfoI SmlI SspBI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII