##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AclI AgeI AlwNI
Aor51HI AscI Asp718I BamHI BbrPI
BbvCI BglI BglII BsaBI BsaI
BsiEI BsiWI BsmBI BspDI BspLU11I
BsrBI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DrdI EagI EclXI Eco47III Eco52I
EcoO65I FseI HpaI KpnI KspI
MamI MluI NaeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SwaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccIII 867 TCCGGA T^CCGGA
AflII 1726 CTTAAG C^TTAAG
AflIII 2734 ACGTGT A^C[GA][CT]GT
AhdI 2807 GACATAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1146 GTGCAC G^TGCAC
ApaLI 1146 GTGCAC G^TGCAC
AspEI 2807 GACATAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2085 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1572 CTCGGG C^[CT]CG[GA]G
AviII 84 TGCGCA TGC^GCA
AvrII 1441 CCTAGG C^CTAGG
BcgI 992 GCATTCCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1726 CTTAAG C^TTAAG
BlnI 1441 CCTAGG C^CTAGG
BlpI 3294 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 3294 GCTAAGC GC^T[ACGT]AGC
BsaWI 867 TCCGGA [AT]^CCGG[AT]
BseAI 867 TCCGGA T^CCGGA
BseRI 295 GAGGAG GAGGAG(10/8)
BsoBI 1572 CTCGGG C^[CT]CG[GA]G
Bsp1407I 3905 TGTACA T^GTACA
BspEI 867 TCCGGA T^CCGGA
BspHI 2421 TCATGA T^CATGA
BsrDI 3148 GCAATG GCAATG(2/0)
BsrFI 3761 GCCGGT [GA]^CCGG[CT]
BsrGI 3905 TGTACA T^GTACA
CelII 3294 GCTAAGC GC^T[ACGT]AGC
Cfr10I 3761 GCCGGT [GA]^CCGG[CT]
DraIII 102 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2807 GACATAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3693 CCTTCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 4000 GAATTC G^AATTC
FspI 84 TGCGCA TGC^GCA
HgaI 182 GCGTC GACGC(5/10)
HincII 2815 GTCAAC GT[CT]^[GA]AC
HindII 2815 GTCAAC GT[CT]^[GA]AC
MfeI 1080 CAATTG C^AATTG
MroI 867 TCCGGA T^CCGGA
MunI 1080 CAATTG C^AATTG
PflFI 2085 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 845 CCAAGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 3688 AGGTCCC [GA]G^G[AT]CC[CT]
RcaI 2421 TCATGA T^CATGA
SspBI 3905 TGTACA T^GTACA
StuI 1369 AGGCCT AGG^CCT
Tth111I 2085 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 845 CCAAGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2215 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
ApaI 1437 GGGCCC GGGCC^C
3021 GGGCCC
AseI 820 ATTAAT AT^TAAT
4220 ATTAAT
AsnI 820 ATTAAT AT^TAAT
4220 ATTAAT
Asp700I 513 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2239 GAAGTGGTTC
AvaII 188 GGACC G^G[AT]CC
3689 GGTCC
BalI 218 TGGCCA TGG^CCA
1695 TGGCCA
BbeI 69 GGCGCC GGCGC^C
1931 GGCGCC
BciVI 1524 GGATAC GTATCC(6/5)
1532 GGATAC
BclI 1034 TGATCA T^GATCA
3570 TGATCA
BsaAI 2733 TACGTG [CT]AC^GT[GA]
2782 TACGTG
BsmI 2479 GAATGC GAATGC(1/-1)
992 GCATTC
BspMI 355 GCAGGT ACCTGC(4/8)
1986 GCAGGT
BstYI 807 GGATCT [GA]^GATC[CT]
1960 AGATCC
EcoRV 1052 GATATC GAT^ATC
1173 GATATC
FbaI 1034 TGATCA T^GATCA
3570 TGATCA
KasI 69 GGCGCC G^GCGCC
1931 GGCGCC
MaeII 2734 ACGT A^CGT
2783 ACGT
MflI 807 GGATCT [GA]^GATC[CT]
1960 AGATCC
MluNI 218 TGGCCA TGG^CCA
1695 TGGCCA
MscI 218 TGGCCA TGG^CCA
1695 TGGCCA
NarI 69 GGCGCC GG^CGCC
1931 GGCGCC
NdeI 2547 CATATG CA^TATG
4005 CATATG
PshBI 820 ATTAAT AT^TAAT
4220 ATTAAT
PstI 2474 CTGCAG CTGCA^G
2907 CTGCAG
SfoI 69 GGCGCC GGC^GCC
1931 GGCGCC
SspI 3604 AATATT AAT^ATT
4223 AATATT
XhoII 807 GGATCT [GA]^GATC[CT]
1960 AGATCC
XmnI 513 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2239 GAAGTGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AocI ApoI
AspHI BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaHI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NsiI
NspI PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI