################################## # NO CUT ENZYMES ( 66 enzymes) # ################################## AatII AccIII AclI AgeI Aor51HI ApaI AscI AseI AsnI AviII AvrII BbeI BciVI BclI BglI BlnI BsaBI BseAI BspDI BspEI BspHI BssHII ClaI CpoI DrdI Eco47III EcoRI EcoRV FbaI FseI FspI HindIII HpaI KasI KspI MamI MluI MroI NaeI NarI NgoMIV NheI NotI NruI PacI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SfiI SfoI SgrAI SpeI SphI Sse8387I SspI StuI SwaI XbaI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AcyI 4055 GACGCC G[GA]^CG[CT]C AflII 1270 CTTAAG C^TTAAG AflIII 1635 ACATGT A^C[GA][CT]GT Alw44I 3906 GTGCAC G^TGCAC ApaLI 3906 GTGCAC G^TGCAC AspI 128 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 1911 CACGTG CAC^GTG BcgI 3115 CGATTACAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 1270 CTTAAG C^TTAAG BglII 2074 AGATCT A^GATCT BlpI 3448 GCTCAGC GC^T[ACGT]AGC Bpu1102I 3448 GCTCAGC GC^T[ACGT]AGC BsaHI 4055 GACGCC G[GA]^CG[CT]C BsaWI 3894 ACCGGA [AT]^CCGG[AT] BsiWI 1538 CGTACG C^GTACG BsmBI 2960 GAGACG CGTCTC(1/5) BsmI 2732 GCATTC GAATGC(1/-1) Bsp1407I 391 TGTACA T^GTACA BspLU11I 1635 ACATGT A^CATGT BsrBI 231 GAGCGG CCGCTC(-3/-3) BsrFI 3351 ACCGGC [GA]^CCGG[CT] BsrGI 391 TGTACA T^GTACA BssSI 1671 CTCGTG CACGAG(-5/-1) Bst1107I 2405 GTATAC GTA^TAC BstBI 459 TTCGAA TT^CGAA BstEII 1153 GGTCACC G^GT[ACGT]ACC BstPI 1153 GGTCACC G^GT[ACGT]ACC BstZ17I 2405 GTATAC GTA^TAC CelII 3448 GCTCAGC GC^T[ACGT]AGC CfoI 87 GCGC GCG^C Cfr10I 3351 ACCGGC [GA]^CCGG[CT] DraI 278 TTTAAA TTT^AAA EcoNI 39 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1153 GGTCACC G^GT[ACGT]ACC EcoT22I 3952 ATGCAT ATGCA^T HaeII 86 GGCGCT [GA]GCGC^[CT] HgaI 4055 GACGC GACGC(5/10) HhaI 87 GCGC GCG^C Hin1I 4055 GACGCC G[GA]^CG[CT]C HinP1I 87 GCGC G^CGC HincII 1776 GTCAAC GT[CT]^[GA]AC HindII 1776 GTCAAC GT[CT]^[GA]AC MfeI 1398 CAATTG C^AATTG MunI 1398 CAATTG C^AATTG NcoI 3219 CCATGG C^CATGG NsiI 3952 ATGCAT ATGCA^T NspV 459 TTCGAA TT^CGAA PflFI 128 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 814 CCAGCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1911 CACGTG CAC^GTG PmlI 1911 CACGTG CAC^GTG PshAI 1475 GACCCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 2949 GAAGAGC GCTCTTC(1/4) SexAI 2281 ACCTGGT A^CC[AT]GGT SfuI 459 TTCGAA TT^CGAA SmaI 3495 CCCGGG CCC^GGG SnaBI 1536 TACGTA TAC^GTA SspBI 391 TGTACA T^GTACA Tth111I 128 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 814 CCAGCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 3495 CCCGGG C^CCGGG XmaI 3495 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### Acc65I 2113 GGTACC G^GTACC 2514 GGTACC AccI 2405 GTATAC GT^[AC][GT]AC 3527 GTAGAC AccII 1 CGCG CG^CG 173 CGCG AhdI 1286 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2997 GACCAACAGTC AlwNI 3290 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG 3986 CAGTATCTG AocI 3080 CCTCAGG CC^T[ACGT]AGG 3185 CCTCAGG Asp700I 452 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1715 GAAAAGTTTC Asp718I 2113 GGTACC G^GTACC 2514 GGTACC AspEI 1286 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2997 GACCAACAGTC BsaAI 1536 TACGTA [CT]AC^GT[GA] 1911 CACGTG BsaI 1419 GAGACC GGTCTC(1/5) 2991 GAGACC BsiEI 106 CGGCCG CG[GA][CT]^CG 187 CGGCCG BstAPI 498 GCAAAATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3985 GCAGTATCTGC BstUI 1 CGCG CG^CG 173 CGCG Bsu36I 3080 CCTCAGG CC^T[ACGT]AGG 3185 CCTCAGG DsaI 109 CCGTGG C^C[GA][CT]GG 3219 CCATGG EagI 106 CGGCCG C^GGCCG 187 CGGCCG Eam1105I 1286 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2997 GACCAACAGTC EclXI 106 CGGCCG C^GGCCG 187 CGGCCG Eco52I 106 CGGCCG C^GGCCG 187 CGGCCG Eco81I 3080 CCTCAGG CC^T[ACGT]AGG 3185 CCTCAGG FauI 2247 CCCGC CCCGC(4/6) 2882 GCGGG KpnI 2113 GGTACC GGTAC^C 2514 GGTACC MvnI 1 CGCG CG^CG 173 CGCG NdeI 1331 CATATG CA^TATG 3126 CATATG NspI 1114 GCATGT [GA]CATG^[CT] 1635 ACATGT PaeR7I 2118 CTCGAG C^TCGAG 2172 CTCGAG SacI 887 GAGCTC GAGCT^C 3132 GAGCTC XcmI 2865 CCAAAGATGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3219 CCATGGCAGAAGTGG XhoI 2118 CTCGAG C^TCGAG 2172 CTCGAG XmnI 452 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1715 GAAAAGTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 99 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI AvaI AvaII BalI BamHI BanI BanII BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraII DraIII EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII