##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AccIII AclI AgeI Aor51HI
ApaI AscI AseI AsnI AviII
AvrII BbeI BciVI BclI BglI
BlnI BsaBI BseAI BspDI BspEI
BspHI BssHII ClaI CpoI DrdI
Eco47III EcoRI EcoRV FbaI FseI
FspI HindIII HpaI KasI KspI
MamI MluI MroI NaeI NarI
NgoMIV NheI NotI NruI PacI
PinAI PmeI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SfiI SfoI SgrAI SpeI
SphI Sse8387I SspI StuI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AcyI 4055 GACGCC G[GA]^CG[CT]C
AflII 1270 CTTAAG C^TTAAG
AflIII 1635 ACATGT A^C[GA][CT]GT
Alw44I 3906 GTGCAC G^TGCAC
ApaLI 3906 GTGCAC G^TGCAC
AspI 128 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1911 CACGTG CAC^GTG
BcgI 3115 CGATTACAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1270 CTTAAG C^TTAAG
BglII 2074 AGATCT A^GATCT
BlpI 3448 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3448 GCTCAGC GC^T[ACGT]AGC
BsaHI 4055 GACGCC G[GA]^CG[CT]C
BsaWI 3894 ACCGGA [AT]^CCGG[AT]
BsiWI 1538 CGTACG C^GTACG
BsmBI 2960 GAGACG CGTCTC(1/5)
BsmI 2732 GCATTC GAATGC(1/-1)
Bsp1407I 391 TGTACA T^GTACA
BspLU11I 1635 ACATGT A^CATGT
BsrBI 231 GAGCGG CCGCTC(-3/-3)
BsrFI 3351 ACCGGC [GA]^CCGG[CT]
BsrGI 391 TGTACA T^GTACA
BssSI 1671 CTCGTG CACGAG(-5/-1)
Bst1107I 2405 GTATAC GTA^TAC
BstBI 459 TTCGAA TT^CGAA
BstEII 1153 GGTCACC G^GT[ACGT]ACC
BstPI 1153 GGTCACC G^GT[ACGT]ACC
BstZ17I 2405 GTATAC GTA^TAC
CelII 3448 GCTCAGC GC^T[ACGT]AGC
CfoI 87 GCGC GCG^C
Cfr10I 3351 ACCGGC [GA]^CCGG[CT]
DraI 278 TTTAAA TTT^AAA
EcoNI 39 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1153 GGTCACC G^GT[ACGT]ACC
EcoT22I 3952 ATGCAT ATGCA^T
HaeII 86 GGCGCT [GA]GCGC^[CT]
HgaI 4055 GACGC GACGC(5/10)
HhaI 87 GCGC GCG^C
Hin1I 4055 GACGCC G[GA]^CG[CT]C
HinP1I 87 GCGC G^CGC
HincII 1776 GTCAAC GT[CT]^[GA]AC
HindII 1776 GTCAAC GT[CT]^[GA]AC
MfeI 1398 CAATTG C^AATTG
MunI 1398 CAATTG C^AATTG
NcoI 3219 CCATGG C^CATGG
NsiI 3952 ATGCAT ATGCA^T
NspV 459 TTCGAA TT^CGAA
PflFI 128 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 814 CCAGCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1911 CACGTG CAC^GTG
PmlI 1911 CACGTG CAC^GTG
PshAI 1475 GACCCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 2949 GAAGAGC GCTCTTC(1/4)
SexAI 2281 ACCTGGT A^CC[AT]GGT
SfuI 459 TTCGAA TT^CGAA
SmaI 3495 CCCGGG CCC^GGG
SnaBI 1536 TACGTA TAC^GTA
SspBI 391 TGTACA T^GTACA
Tth111I 128 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 814 CCAGCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 3495 CCCGGG C^CCGGG
XmaI 3495 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 2113 GGTACC G^GTACC
2514 GGTACC
AccI 2405 GTATAC GT^[AC][GT]AC
3527 GTAGAC
AccII 1 CGCG CG^CG
173 CGCG
AhdI 1286 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2997 GACCAACAGTC
AlwNI 3290 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
3986 CAGTATCTG
AocI 3080 CCTCAGG CC^T[ACGT]AGG
3185 CCTCAGG
Asp700I 452 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1715 GAAAAGTTTC
Asp718I 2113 GGTACC G^GTACC
2514 GGTACC
AspEI 1286 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2997 GACCAACAGTC
BsaAI 1536 TACGTA [CT]AC^GT[GA]
1911 CACGTG
BsaI 1419 GAGACC GGTCTC(1/5)
2991 GAGACC
BsiEI 106 CGGCCG CG[GA][CT]^CG
187 CGGCCG
BstAPI 498 GCAAAATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3985 GCAGTATCTGC
BstUI 1 CGCG CG^CG
173 CGCG
Bsu36I 3080 CCTCAGG CC^T[ACGT]AGG
3185 CCTCAGG
DsaI 109 CCGTGG C^C[GA][CT]GG
3219 CCATGG
EagI 106 CGGCCG C^GGCCG
187 CGGCCG
Eam1105I 1286 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2997 GACCAACAGTC
EclXI 106 CGGCCG C^GGCCG
187 CGGCCG
Eco52I 106 CGGCCG C^GGCCG
187 CGGCCG
Eco81I 3080 CCTCAGG CC^T[ACGT]AGG
3185 CCTCAGG
FauI 2247 CCCGC CCCGC(4/6)
2882 GCGGG
KpnI 2113 GGTACC GGTAC^C
2514 GGTACC
MvnI 1 CGCG CG^CG
173 CGCG
NdeI 1331 CATATG CA^TATG
3126 CATATG
NspI 1114 GCATGT [GA]CATG^[CT]
1635 ACATGT
PaeR7I 2118 CTCGAG C^TCGAG
2172 CTCGAG
SacI 887 GAGCTC GAGCT^C
3132 GAGCTC
XcmI 2865 CCAAAGATGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3219 CCATGGCAGAAGTGG
XhoI 2118 CTCGAG C^TCGAG
2172 CTCGAG
XmnI 452 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1715 GAAAAGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaI AvaII BalI
BamHI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
Cfr13I DdeI DpnII DraII DraIII
EaeI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII