##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AflIII AgeI AhdI AscI AseI
AsnI Asp700I AspEI AspI AviII
BamHI BbeI BbrPI BciVI BclI
BfrI BlpI Bpu1102I BsaBI BsaHI
BseAI BsiWI BsmBI Bsp1407I BspDI
BspEI BspLU11I BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI DraIII DrdI
Eam1105I EcoO65I EcoRV FbaI FseI
FspI Hin1I HpaI KasI MamI
MfeI MluI MroI MunI NarI
NdeI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SalI SapI ScaI SfoI SfuI
SgrAI SnaBI SspBI SspI SwaI
Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 2294 GGTACC G^GTACC
Alw44I 369 GTGCAC G^TGCAC
Aor51HI 1406 AGCGCT AGC^GCT
ApaI 3498 GGGCCC GGGCC^C
ApaLI 369 GTGCAC G^TGCAC
Asp718I 2294 GGTACC G^GTACC
AvrII 508 CCTAGG C^CTAGG
BbsI 3319 GAAGAC GAAGAC(2/6)
BcgI 408 GCAGCAGTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1014 AGATCT A^GATCT
BlnI 508 CCTAGG C^CTAGG
BpuAI 3319 GAAGAC GAAGAC(2/6)
BsaAI 2289 TACGTG [CT]AC^GT[GA]
BsgI 1249 GTGCAG GTGCAG(16/14)
BsiEI 207 CGGCCG CG[GA][CT]^CG
BstAPI 2251 GCAGGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI 4134 TTTAAA TTT^AAA
EagI 207 CGGCCG C^GGCCG
EclXI 207 CGGCCG C^GGCCG
Eco47III 1406 AGCGCT AGC^GCT
Eco52I 207 CGGCCG C^GGCCG
EcoRI 1724 GAATTC G^AATTC
HaeII 1406 AGCGCT [GA]GCGC^[CT]
HgaI 2608 GCGTC GACGC(5/10)
HincII 3647 GTCAAC GT[CT]^[GA]AC
HindII 3647 GTCAAC GT[CT]^[GA]AC
KpnI 2294 GGTACC GGTAC^C
NheI 770 GCTAGC G^CTAGC
NotI 206 GCGGCCGC GC^GGCCGC
SacI 917 GAGCTC GAGCT^C
SpeI 3060 ACTAGT A^CTAGT
SphI 454 GCATGC GCATG^C
XbaI 2047 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 1724 GAATTC [GA]^AATT[CT]
4124 AAATTT
ApoI 1724 GAATTC [GA]^AATT[CT]
4124 AAATTT
BbvCI 2879 CCTCAGC CCTCAGC(-5/-2)
1717 GCTGAGG
BsaWI 52 ACCGGA [AT]^CCGG[AT]
273 TCCGGT
BsmI 937 GAATGC GAATGC(1/-1)
3892 GAATGC
BsrBI 970 CCGCTC CCGCTC(-3/-3)
543 GAGCGG
EcoT22I 2910 ATGCAT ATGCA^T
3044 ATGCAT
HindIII 1446 AAGCTT A^AGCTT
2042 AAGCTT
MaeII 681 ACGT A^CGT
2290 ACGT
NaeI 1316 GCCGGC GCC^GGC
1835 GCCGGC
NgoMIV 1316 GCCGGC G^CCGGC
1835 GCCGGC
NsiI 2910 ATGCAT ATGCA^T
3044 ATGCAT
PflMI 1376 CCAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2563 CCAGCCACTGG
SbfI 1960 CCTGCAGG CCTGCA^GG
3677 CCTGCAGG
SexAI 990 ACCTGGT A^CC[AT]GGT
1302 ACCTGGT
SfiI 592 GGCCAGCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1864 GGCCTGGGTGGCC
Sse8387I 1960 CCTGCAGG CCTGCA^GG
3677 CCTGCAGG
TaqI 2858 TCGA T^CGA
3486 TCGA
TthHB8I 2858 TCGA T^CGA
3486 TCGA
Van91I 1376 CCAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2563 CCAGCCACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AccII AciI AfaI AluI
AlwI AlwNI AocI AspHI AvaI
AvaII BalI BanI BanII BbvI
BcnI BfaI BglI BmyI BpmI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspHI BspMI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I KspI
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RcaI RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XcmI XhoII
XmaCI XmaI