##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccIII AgeI Aor51HI
AscI Asp718I BbeI BbrPI BclI
BglI BlpI Bpu1102I BsaWI BseAI
BsiEI BsiWI BsmI BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BstEII
BstPI CelII Cfr10I ClaI CpoI
DraIII EagI EclXI Eco47III Eco52I
EcoO65I EcoRV EcoT22I FbaI FseI
HaeII KasI KpnI KspI MluI
MroI NaeI NarI NcoI NgoMIV
NotI NruI NsiI PacI PinAI
PmaCI PmeI PmlI PvuI RcaI
RsrII SacI SacII SbfI SexAI
SfiI SfoI SgrAI SnaBI SphI
Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AclI 3117 AACGTT AA^CGTT
AflII 3576 CTTAAG C^TTAAG
AhdI 2438 GACTGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2662 GTGCAC G^TGCAC
AocI 3717 CCTGAGG CC^T[ACGT]AGG
ApaLI 2662 GTGCAC G^TGCAC
AspEI 2438 GACTGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2044 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 2386 GTCTTC GAAGAC(2/6)
BcgI 2191 CGAGCATCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3576 CTTAAG C^TTAAG
BpuAI 2386 GTCTTC GAAGAC(2/6)
BsaAI 2905 CACGTA [CT]AC^GT[GA]
BsaBI 3162 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrDI 3621 GCAATG GCAATG(2/0)
BssHII 1202 GCGCGC G^CGCGC
BssSI 3046 CTCGTG CACGAG(-5/-1)
Bst1107I 3628 GTATAC GTA^TAC
BstBI 2113 TTCGAA TT^CGAA
BstZ17I 3628 GTATAC GTA^TAC
Bsu36I 3717 CCTGAGG CC^T[ACGT]AGG
DraI 494 TTTAAA TTT^AAA
DrdI 3878 GACTACCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 272 CCGTGG C^C[GA][CT]GG
Eam1105I 2438 GACTGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3717 CCTGAGG CC^T[ACGT]AGG
EcoNI 4362 CCTGTGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
MamI 3162 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4144 CAATTG C^AATTG
MunI 4144 CAATTG C^AATTG
NdeI 3801 CATATG CA^TATG
NheI 121 GCTAGC G^CTAGC
NspI 4282 ACATGC [GA]CATG^[CT]
NspV 2113 TTCGAA TT^CGAA
PaeR7I 1110 CTCGAG C^TCGAG
PflFI 2044 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1436 CCAGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 2479 GACAGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3117 AACGTT AA^CGTT
SalI 2152 GTCGAC G^TCGAC
SfuI 2113 TTCGAA TT^CGAA
SmaI 1476 CCCGGG CCC^GGG
SpeI 3033 ACTAGT A^CTAGT
StuI 1523 AGGCCT AGG^CCT
Tth111I 2044 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1436 CCAGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 880 TCTAGA T^CTAGA
XcmI 4263 CCAAAATTTGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1110 CTCGAG C^TCGAG
XmaCI 1476 CCCGGG C^CCGGG
XmaI 1476 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 693 GGCGTC G[GA]^CG[CT]C
972 GACGCC
AflIII 1180 ACACGT A^C[GA][CT]GT
2904 ACACGT
Asp700I 2298 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4135 GAAAGGGTTC
AviII 1077 TGCGCA TGC^GCA
1138 TGCGCA
AvrII 3066 CCTAGG C^CTAGG
3652 CCTAGG
BglII 98 AGATCT A^GATCT
170 AGATCT
BlnI 3066 CCTAGG C^CTAGG
3652 CCTAGG
BsaHI 693 GGCGTC G[GA]^CG[CT]C
972 GACGCC
BsmBI 1183 CGTCTC CGTCTC(1/5)
1164 GAGACG
Bsp1407I 487 TGTACA T^GTACA
4279 TGTACA
BsrGI 487 TGTACA T^GTACA
4279 TGTACA
BstAPI 1141 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3612 GCACAGATTGC
EcoRI 2266 GAATTC G^AATTC
3657 GAATTC
FspI 1077 TGCGCA TGC^GCA
1138 TGCGCA
Hin1I 693 GGCGTC G[GA]^CG[CT]C
972 GACGCC
HindIII 3561 AAGCTT A^AGCTT
3695 AAGCTT
HpaI 2523 GTTAAC GTT^AAC
3567 GTTAAC
PpuMI 337 AGGACCT [GA]G^G[AT]CC[CT]
3327 AGGTCCT
SapI 2985 GAAGAGC GCTCTTC(1/4)
370 GCTCTTC
ScaI 664 AGTACT AGT^ACT
3030 AGTACT
SspBI 487 TGTACA T^GTACA
4279 TGTACA
XmnI 2298 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4135 GAAAGGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApaI ApoI
AseI AsnI AspHI AvaI AvaII
BalI BamHI BanI BanII BbvCI
BbvI BciVI BcnI BfaI BmyI
BpmI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PshBI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII