################################## # NO CUT ENZYMES ( 77 enzymes) # ################################## Acc65I AccI AclI AcsI AflII AgeI Alw44I ApaLI ApoI AscI AseI AsnI Asp718I AvrII BbeI BcgI BciVI BfrI BlnI BsiEI BsiWI BspDI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI DraI EagI EclXI Eco52I EcoO65I EcoRI FseI HincII HindII HpaI KasI KpnI KspI MfeI MluI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI XhoI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccII 1006 CGCG CG^CG AccIII 2 TCCGGA T^CCGGA Asp700I 427 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 2973 TGCGCA TGC^GCA BamHI 2069 GGATCC G^GATCC BbrPI 3080 CACGTG CAC^GTG BbvCI 1025 GCTGAGG CCTCAGC(-5/-2) BclI 3708 TGATCA T^GATCA BglII 1512 AGATCT A^GATCT BsaWI 2 TCCGGA [AT]^CCGG[AT] BseAI 2 TCCGGA T^CCGGA BsmBI 415 CGTCTC CGTCTC(1/5) BsmI 981 GCATTC GAATGC(1/-1) BspEI 2 TCCGGA T^CCGGA BspLU11I 41 ACATGT A^CATGT BsrDI 3648 CATTGC GCAATG(2/0) BsrFI 7 ACCGGC [GA]^CCGG[CT] BstUI 1006 CGCG CG^CG Cfr10I 7 ACCGGC [GA]^CCGG[CT] CpoI 4 CGGACCG CG^G[AT]CCG DraIII 3080 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoT22I 624 ATGCAT ATGCA^T FbaI 3708 TGATCA T^GATCA FspI 2973 TGCGCA TGC^GCA MroI 2 TCCGGA T^CCGGA MvnI 1006 CGCG CG^CG NcoI 2091 CCATGG C^CATGG NsiI 624 ATGCAT ATGCA^T PflMI 3178 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 3080 CACGTG CAC^GTG PmlI 3080 CACGTG CAC^GTG RsrII 4 CGGACCG CG^G[AT]CCG SexAI 3215 ACCTGGT A^CC[AT]GGT Van91I 3178 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 3385 TCTAGA T^CTAGA XmnI 427 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AatII 3047 GACGTC GACGT^C 3104 GACGTC AflIII 41 ACATGT A^C[GA][CT]GT 1984 ACACGT AhdI 40 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3314 GACCTGAGGTC AocI 3052 CCTCAGG CC^T[ACGT]AGG 3316 CCTGAGG Aor51HI 67 AGCGCT AGC^GCT 1281 AGCGCT AspEI 40 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3314 GACCTGAGGTC AspI 2204 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2774 GACATGGTC BalI 1194 TGGCCA TGG^CCA 2682 TGGCCA BanI 1321 GGTGCC G^G[CT][GA]CC 3356 GGCACC BfaI 2114 CTAG C^TAG 3386 CTAG BglI 1658 GCCAAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3122 GCCCAGCTGGC BsaAI 2990 CACGTA [CT]AC^GT[GA] 3080 CACGTG BsaBI 344 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3059 GATCTCTATC Bsp1407I 585 TGTACA T^GTACA 857 TGTACA BsrBI 1436 CCGCTC CCGCTC(-3/-3) 1916 GAGCGG BsrGI 585 TGTACA T^GTACA 857 TGTACA Bsu36I 3052 CCTCAGG CC^T[ACGT]AGG 3316 CCTGAGG DrdI 430 GACCTTCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2343 GACAAACCAGTC Eam1105I 40 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3314 GACCTGAGGTC Eco47III 67 AGCGCT AGC^GCT 1281 AGCGCT Eco81I 3052 CCTCAGG CC^T[ACGT]AGG 3316 CCTGAGG EcoRV 266 GATATC GAT^ATC 3558 GATATC HindIII 2579 AAGCTT A^AGCTT 3422 AAGCTT MaeI 2114 CTAG C^TAG 3386 CTAG MamI 344 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3059 GATCTCTATC MluNI 1194 TGGCCA TGG^CCA 2682 TGGCCA MscI 1194 TGGCCA TGG^CCA 2682 TGGCCA PflFI 2204 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2774 GACATGGTC SmlI 103 CTCAAG C^T[CT][GA]AG 1874 CTTGAG SphI 622 GCATGC GCATG^C 3118 GCATGC SspBI 585 TGTACA T^GTACA 857 TGTACA StuI 2038 AGGCCT AGG^CCT 3488 AGGCCT Tth111I 2204 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2774 GACATGGTC ######################################### # 3 OR MORE CUT ENZYMES ( 112 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BanII BbsI BbvI BcnI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspMI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmaCI XmaI