##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AgeI Alw44I ApaLI ApoI AscI
AseI AsnI Asp718I AvrII BbeI
BcgI BciVI BfrI BlnI BsiEI
BsiWI BspDI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
DraI EagI EclXI Eco52I EcoO65I
EcoRI FseI HincII HindII HpaI
KasI KpnI KspI MfeI MluI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccII 1006 CGCG CG^CG
AccIII 2 TCCGGA T^CCGGA
Asp700I 427 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 2973 TGCGCA TGC^GCA
BamHI 2069 GGATCC G^GATCC
BbrPI 3080 CACGTG CAC^GTG
BbvCI 1025 GCTGAGG CCTCAGC(-5/-2)
BclI 3708 TGATCA T^GATCA
BglII 1512 AGATCT A^GATCT
BsaWI 2 TCCGGA [AT]^CCGG[AT]
BseAI 2 TCCGGA T^CCGGA
BsmBI 415 CGTCTC CGTCTC(1/5)
BsmI 981 GCATTC GAATGC(1/-1)
BspEI 2 TCCGGA T^CCGGA
BspLU11I 41 ACATGT A^CATGT
BsrDI 3648 CATTGC GCAATG(2/0)
BsrFI 7 ACCGGC [GA]^CCGG[CT]
BstUI 1006 CGCG CG^CG
Cfr10I 7 ACCGGC [GA]^CCGG[CT]
CpoI 4 CGGACCG CG^G[AT]CCG
DraIII 3080 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT22I 624 ATGCAT ATGCA^T
FbaI 3708 TGATCA T^GATCA
FspI 2973 TGCGCA TGC^GCA
MroI 2 TCCGGA T^CCGGA
MvnI 1006 CGCG CG^CG
NcoI 2091 CCATGG C^CATGG
NsiI 624 ATGCAT ATGCA^T
PflMI 3178 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3080 CACGTG CAC^GTG
PmlI 3080 CACGTG CAC^GTG
RsrII 4 CGGACCG CG^G[AT]CCG
SexAI 3215 ACCTGGT A^CC[AT]GGT
Van91I 3178 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3385 TCTAGA T^CTAGA
XmnI 427 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AatII 3047 GACGTC GACGT^C
3104 GACGTC
AflIII 41 ACATGT A^C[GA][CT]GT
1984 ACACGT
AhdI 40 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3314 GACCTGAGGTC
AocI 3052 CCTCAGG CC^T[ACGT]AGG
3316 CCTGAGG
Aor51HI 67 AGCGCT AGC^GCT
1281 AGCGCT
AspEI 40 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3314 GACCTGAGGTC
AspI 2204 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2774 GACATGGTC
BalI 1194 TGGCCA TGG^CCA
2682 TGGCCA
BanI 1321 GGTGCC G^G[CT][GA]CC
3356 GGCACC
BfaI 2114 CTAG C^TAG
3386 CTAG
BglI 1658 GCCAAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3122 GCCCAGCTGGC
BsaAI 2990 CACGTA [CT]AC^GT[GA]
3080 CACGTG
BsaBI 344 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3059 GATCTCTATC
Bsp1407I 585 TGTACA T^GTACA
857 TGTACA
BsrBI 1436 CCGCTC CCGCTC(-3/-3)
1916 GAGCGG
BsrGI 585 TGTACA T^GTACA
857 TGTACA
Bsu36I 3052 CCTCAGG CC^T[ACGT]AGG
3316 CCTGAGG
DrdI 430 GACCTTCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2343 GACAAACCAGTC
Eam1105I 40 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3314 GACCTGAGGTC
Eco47III 67 AGCGCT AGC^GCT
1281 AGCGCT
Eco81I 3052 CCTCAGG CC^T[ACGT]AGG
3316 CCTGAGG
EcoRV 266 GATATC GAT^ATC
3558 GATATC
HindIII 2579 AAGCTT A^AGCTT
3422 AAGCTT
MaeI 2114 CTAG C^TAG
3386 CTAG
MamI 344 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3059 GATCTCTATC
MluNI 1194 TGGCCA TGG^CCA
2682 TGGCCA
MscI 1194 TGGCCA TGG^CCA
2682 TGGCCA
PflFI 2204 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2774 GACATGGTC
SmlI 103 CTCAAG C^T[CT][GA]AG
1874 CTTGAG
SphI 622 GCATGC GCATG^C
3118 GCATGC
SspBI 585 TGTACA T^GTACA
857 TGTACA
StuI 2038 AGGCCT AGG^CCT
3488 AGGCCT
Tth111I 2204 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2774 GACATGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BanII BbsI BbvI BcnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspHI BspMI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RcaI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmaCI XmaI