##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AccIII AflII AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AspEI
AviII AvrII BbeI BbrPI BciVI
BfrI BlnI BpmI BsaAI BsaBI
BseAI BsgI BsiWI BsmBI BspDI
BspEI BsrBI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CfoI ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV FauI FseI
FspI HaeII HhaI HinP1I HindIII
KasI KspI MamI MfeI MluI
MroI MunI NaeI NarI NgoMIV
NheI NotI NruI NspV PacI
PmaCI PmeI PmlI PvuI RsrII
SacII SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI Sse8387I
StuI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 902 GACGTC GACGT^C
Acc65I 3695 GGTACC G^GTACC
AccII 4626 CGCG CG^CG
AclI 948 AACGTT AA^CGTT
AgeI 2262 ACCGGT A^CCGGT
Asp718I 3695 GGTACC G^GTACC
AspHI 248 GAGCTC G[AT]GC[AT]^C
AspI 1990 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 3781 TGGCCA TGG^CCA
BamHI 3467 GGATCC G^GATCC
BanI 3695 GGTACC G^G[CT][GA]CC
BbvCI 2657 CCTCAGC CCTCAGC(-5/-2)
BcgI 971 GCACATGTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 3578 GCCTCCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1544 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1544 GCTGAGC GC^T[ACGT]AGC
BsiEI 2264 CGGTCG CG[GA][CT]^CG
BsiHKAI 248 GAGCTC G[AT]GC[AT]^C
Bsp1407I 4597 TGTACA T^GTACA
BspHI 2732 TCATGA T^CATGA
BspMI 310 GCAGGT ACCTGC(4/8)
BsrFI 2262 ACCGGT [GA]^CCGG[CT]
BsrGI 4597 TGTACA T^GTACA
BstAPI 3587 GCAAGGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 4626 CGCG CG^CG
CelII 1544 GCTGAGC GC^T[ACGT]AGC
Cfr10I 2262 ACCGGT [GA]^CCGG[CT]
DraIII 619 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 3781 TGGCCA [CT]^GGCC[GA]
EcoRI 1529 GAATTC G^AATTC
EcoT22I 877 ATGCAT ATGCA^T
HpaI 1489 GTTAAC GTT^AAC
KpnI 3695 GGTACC GGTAC^C
MluNI 3781 TGGCCA TGG^CCA
MscI 3781 TGGCCA TGG^CCA
MvnI 4626 CGCG CG^CG
NsiI 877 ATGCAT ATGCA^T
PflFI 1990 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2262 ACCGGT A^CCGGT
PpuMI 3531 GGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 948 AACGTT AA^CGTT
PstI 2406 CTGCAG CTGCA^G
RcaI 2732 TCATGA T^CATGA
SacI 248 GAGCTC GAGCT^C
ScaI 1199 AGTACT AGT^ACT
SmaI 3605 CCCGGG CCC^GGG
SphI 4094 GCATGC GCATG^C
SspBI 4597 TGTACA T^GTACA
Tth111I 1990 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 3573 CCACCGCCTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 3605 CCCGGG C^CCGGG
XmaI 3605 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflIII 973 ACATGT A^C[GA][CT]GT
1516 ACATGT
AlwNI 1654 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
1664 CAGTACCTG
AseI 534 ATTAAT AT^TAAT
3248 ATTAAT
AsnI 534 ATTAAT AT^TAAT
3248 ATTAAT
Asp700I 875 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2117 GAAGTTTTTC
BanII 248 GAGCTC G[GA]GC[CT]^C
2023 GAGCCC
BclI 1711 TGATCA T^GATCA
2051 TGATCA
BsaWI 2262 ACCGGT [AT]^CCGG[AT]
3692 TCCGGT
BspLU11I 973 ACATGT A^CATGT
1516 ACATGT
DraII 2188 AGGGCCT [GA]G^G[ACGT]CC[CT]
3531 GGGTCCT
EcoO109I 2188 AGGGCCT [GA]G^G[ACGT]CC[CT]
3531 GGGTCCT
FbaI 1711 TGATCA T^GATCA
2051 TGATCA
NdeI 305 CATATG CA^TATG
1353 CATATG
PaeR7I 251 CTCGAG C^TCGAG
3805 CTCGAG
PshAI 3870 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4283 GACCTTGGTC
PshBI 534 ATTAAT AT^TAAT
3248 ATTAAT
SalI 3376 GTCGAC G^TCGAC
4043 GTCGAC
SfcI 2406 CTGCAG C^T[GA][CT]AG
4324 CTATAG
SpeI 1069 ACTAGT A^CTAGT
2892 ACTAGT
XhoI 251 CTCGAG C^TCGAG
3805 CTCGAG
XmnI 875 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2117 GAAGTTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI ApoI AvaI AvaII
BbsI BbvI BcnI BfaI BglII
BmyI BpuAI BsaHI BsaI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI DsaI
EarI EcoNI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI Hin1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII