##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AclI AgeI AscI
AseI AsnI Asp718I AviII AvrII
BcgI BclI BlnI BsaBI BsiEI
BsiWI BsmBI Bsp1407I BspDI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraIII EagI
EclXI Eco52I EcoO65I EcoRV EcoT22I
FbaI FseI FspI KpnI MamI
MfeI MluI MunI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RsrII
SalI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
StuI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccIII 2611 TCCGGA T^CCGGA
AhdI 3975 GACTAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 3975 GACTAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3654 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 3214 TGGCCA TGG^CCA
BbrPI 799 CACGTG CAC^GTG
BciVI 794 GGATAC GTATCC(6/5)
BglII 3141 AGATCT A^GATCT
BsaAI 799 CACGTG [CT]AC^GT[GA]
BsaWI 2611 TCCGGA [AT]^CCGG[AT]
BseAI 2611 TCCGGA T^CCGGA
BsmI 2456 GAATGC GAATGC(1/-1)
BspEI 2611 TCCGGA T^CCGGA
BspHI 4916 TCATGA T^CATGA
BsrFI 2839 GCCGGC [GA]^CCGG[CT]
BssSI 1770 CTCGTG CACGAG(-5/-1)
Cfr10I 2839 GCCGGC [GA]^CCGG[CT]
DrdI 2938 GACTGCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3975 GACTAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HpaI 3074 GTTAAC GTT^AAC
KspI 839 CCGCGG CCGC^GG
MluNI 3214 TGGCCA TGG^CCA
MroI 2611 TCCGGA T^CCGGA
MscI 3214 TGGCCA TGG^CCA
MslI 510 CACTTGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 2839 GCCGGC GCC^GGC
NdeI 4071 CATATG CA^TATG
NgoMIV 2839 GCCGGC G^CCGGC
PflFI 3654 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 799 CACGTG CAC^GTG
PmlI 799 CACGTG CAC^GTG
PshAI 4220 GACATAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 4916 TCATGA T^CATGA
SacII 839 CCGCGG CCGC^GG
SphI 1708 GCATGC GCATG^C
Tth111I 3654 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflII 1461 CTTAAG C^TTAAG
3829 CTTAAG
Alw44I 406 GTGCAC G^TGCAC
3885 GTGCAC
Aor51HI 2114 AGCGCT AGC^GCT
3243 AGCGCT
ApaLI 406 GTGCAC G^TGCAC
3885 GTGCAC
BbeI 250 GGCGCC GGCGC^C
724 GGCGCC
BfrI 1461 CTTAAG C^TTAAG
3829 CTTAAG
BglI 2493 GCCAAGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2805 GCCAGTGAGGC
BspLU11I 128 ACATGT A^CATGT
1661 ACATGT
BsrDI 3677 CATTGC GCAATG(2/0)
3870 GCAATG
DraI 4922 TTTAAA TTT^AAA
4979 TTTAAA
EaeI 3095 TGGCCG [CT]^GGCC[GA]
3214 TGGCCA
Eco47III 2114 AGCGCT AGC^GCT
3243 AGCGCT
EcoRI 2313 GAATTC G^AATTC
2822 GAATTC
HincII 3074 GTTAAC GT[CT]^[GA]AC
3399 GTCAAC
HindII 3074 GTTAAC GT[CT]^[GA]AC
3399 GTCAAC
HindIII 3589 AAGCTT A^AGCTT
4046 AAGCTT
KasI 250 GGCGCC G^GCGCC
724 GGCGCC
MaeII 800 ACGT A^CGT
3658 ACGT
NarI 250 GGCGCC GG^CGCC
724 GGCGCC
NheI 2711 GCTAGC G^CTAGC
3246 GCTAGC
SapI 1851 GAAGAGC GCTCTTC(1/4)
3005 GAAGAGC
SexAI 1987 ACCAGGT A^CC[AT]GGT
2089 ACCAGGT
SfoI 250 GGCGCC GGC^GCC
724 GGCGCC
XbaI 1538 TCTAGA T^CTAGA
2948 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
AocI ApaI ApoI Asp700I AspHI
AvaI AvaII BamHI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI XmnI